Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 0 | 1 | 3.16 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IEBELMDM_11 | 320850 | 322775 | tet(Q) | ARO:3000191 | tet(Q) | 97.35 | 97.56 | Z21523.1 |
| IEBELMDM_9 | 51651 | 52856 | Mef(En2) | ARO:3004659 | Mef(En2) | 99.25 | 100.00 | AF251288.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IEBELMDM_11 | 320850 | 322775 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 97.09 | 100.00 | L33696 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IEBELMDM_9 | 52881 | 53390 | lnu(AN2) | lincosamide nucleotidyltransferase Lnu(AN2) | BLASTX | 99.41 | 100.00 | WP_004308783.1 |
| IEBELMDM_9 | 51651 | 52853 | mef(En2) | macrolide efflux MFS transporter Mef(En2) | BLASTX | 99.25 | 100.00 | WP_063853729.1 |
| IEBELMDM_11 | 320850 | 322772 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 97.35 | 100.00 | WP_002560998.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IEBELMDM_8 | 121474 | 123054 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 86.91 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IEBELMDM_1 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_2 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_3 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_4 | 1.0 | 0.0 | 1.0 |
| IEBELMDM_5 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_6 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_7 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_8 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_9 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_10 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_11 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_12 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_13 | 1.0 | 0.0 | 1.0 |
| IEBELMDM_14 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_15 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_16 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_17 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_18 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_19 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_20 | 0.0 | 1.0 | 0.0 |
| IEBELMDM_21 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IEBELMDM_7 | 386549 | 397205 | False | Siphoviridae | VOG8171, |
| IEBELMDM_20 | 42078 | 46988 | True | Siphoviridae | VOG0275, |
Phigaro v2.4.0 (Database: Jan. 2024 release)