safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	6	17.46

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
AOHADFFH_1	2406207	2407127	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
AOHADFFH_1	2392879	2394312	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
AOHADFFH_1	2343216	2343809	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
AOHADFFH_1	2113558	2115201	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
AOHADFFH_1	1639813	1640667	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
AOHADFFH_1	1621104	1622306	blt	ARO:3003006	blt	100.00	100.00	L32599.1
AOHADFFH_1	1398652	1399821	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
AOHADFFH_1	425744	426061	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
AOHADFFH_1	425406	425744	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
AOHADFFH_5	27528	30098	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
AOHADFFH_1	1639813	1640667	aadK	Streptomycin	100.00	100.00	M26879
AOHADFFH_1	2406207	2407127	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
AOHADFFH_4	1012482	1013858	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
AOHADFFH_1	1639816	1640667	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
AOHADFFH_1	2406210	2407127	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
AOHADFFH_4	1009964	1010482	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
AOHADFFH_4	1012485	1013858	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
AOHADFFH_1	2113558	2115198	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
AOHADFFH_1	1089720	1092314	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
AOHADFFH_4	522623	523765	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
AOHADFFH_4	524248	525429	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
AOHADFFH_4	523784	524233	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
AOHADFFH_1	1043062	1044825	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
AOHADFFH_4	116313	117098	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
AOHADFFH_4	112477	113415	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
AOHADFFH_4	115091	116287	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
AOHADFFH_4	113443	115062	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
AOHADFFH_4	105321	112457	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
AOHADFFH_1	1199285	1199926	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
AOHADFFH_6	31951	33087	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
AOHADFFH_8	11461	10592	pdxS	1.14e-151	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
AOHADFFH_1	739009	739785	CodY	2.03e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
AOHADFFH_4	371036	371605	ClpP	6.32e-99	part of proteolytic complex	81.05	97.44	BAB94595
AOHADFFH_1	276583	276975	spxA1	6.03e-69	redox-responsive transcription factor	83.21	100.00	AAF21893
AOHADFFH_7	21042	21284	SpoVG	3.95e-38	RNA-binding protein	80.25	79.41	AAT02994

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
AOHADFFH_1	0.0	1.0	0.0
AOHADFFH_4	0.0	1.0	0.0
AOHADFFH_5	0.0	1.0	0.0
AOHADFFH_6	0.0	1.0	0.0
AOHADFFH_7	0.0	1.0	0.0
AOHADFFH_8	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
AOHADFFH_1	369459	395567	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
AOHADFFH_1	1570970	1601347	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)