Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 6 | 9 | 8 | 13.45 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EHCKGBHM_156 | 246793 | 248718 | tet(Q) | ARO:3000191 | tet(Q) | 89.08 | 97.56 | Z21523.1 |
| EHCKGBHM_156 | 232447 | 233247 | ErmF | ARO:3000498 | ErmF | 98.50 | 100.00 | M17124.1 |
| EHCKGBHM_117 | 104488 | 105378 | CblA-1 | ARO:3002999 | CblA-1 | 100.00 | 100.00 | GQ343019.1 |
| EHCKGBHM_30 | 63007 | 64932 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
| EHCKGBHM_40 | 107 | 1291 | Escherichia coli EF-Tu mutants conferring resistance to Pulvomycin | ARO:3003369 | Ecol_EFTu_PLV | 99.75 | 96.33 | AE014075.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EHCKGBHM_30 | 63007 | 64932 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.84 | 100.00 | L33696 |
| EHCKGBHM_156 | 232447 | 233247 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.50 | 100.00 | M17808 |
| EHCKGBHM_30 | 12437 | 13387 | cfxA3 | Ampicillin | 86.12 | 98.34 | AF472622 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EHCKGBHM_117 | 104488 | 105375 | cblA | CblA family class A beta-lactamase | EXACTX | 100.00 | 100.00 | WP_005827792.1 |
| EHCKGBHM_30 | 63007 | 64929 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
| EHCKGBHM_156 | 232447 | 233244 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | BLASTX | 98.87 | 100.00 | WP_002682030.1 |
| EHCKGBHM_156 | 246793 | 248715 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 89.55 | 100.00 | WP_063856407.1 |
| EHCKGBHM_30 | 12436 | 13389 | cfxA3 | extended-spectrum class A beta-lactamase CfxA3 | BLASTX | 83.65 | 99.07 | WP_004348227.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EHCKGBHM_99 | 4488 | 6134 | cka | 0.0 | Colicin K | Exotoxin | 99.82 | 100.00 | CAA61099 |
| EHCKGBHM_141 | 2056 | 3231 | senB | 0.0 | ShET2 | Exotoxin | 99.66 | 100.00 | WP_001020414 |
| EHCKGBHM_50 | 1031 | 1531 | aec17 | 0.0 | EHS | Effector delivery system | 99.60 | 100.00 | WP_000037399 |
| EHCKGBHM_50 | 1031 | 1531 | tssB | 0.0 | T6SS | Effector delivery system | 98.20 | 100.00 | WP_000037389 |
| EHCKGBHM_71 | 1 | 1231 | kpsD | 0.0 | K1 capsule | Invasion | 97.11 | 74.24 | WP_001331697 |
| EHCKGBHM_131 | 12 | 578 | EC042_RS30855 | 0.0 | AAI/SCI-II T6SS | Effector delivery system | 95.77 | 100.00 | WP_000587872 |
| EHCKGBHM_116 | 592 | 2157 | ECSMS35_RS25765 | 0.0 | Colicin E1 | Exotoxin | 95.21 | 99.94 | WP_000447001 |
| EHCKGBHM_21 | 187 | 1680 | gspE | 0.0 | T2SS | Effector delivery system | 94.85 | 99.93 | WP_000249362 |
| EHCKGBHM_50 | 772 | 996 | Z_RS01215 | 2.96e-115 | EHS | Effector delivery system | 100.00 | 100.00 | WP_000123970 |
| EHCKGBHM_88 | 781 | 1398 | fimH | 0.0 | Type 1 fimbriae | Adherence | 97.73 | 68.44 | WP_000832235 |
| EHCKGBHM_99 | 5245 | 6134 | cfa | 0.0 | Col5 | Exotoxin | 88.69 | 60.22 | CAA61102 |
| EHCKGBHM_99 | 5245 | 6134 | cta | 0.0 | Col10 | Exotoxin | 80.98 | 60.15 | CAA57998 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EHCKGBHM_141 | 2056 | 3231 | virulence_ecoli | senB | Plasmid-encoded enterotoxin | 100.0 | 100.00 | CP000038 |
| EHCKGBHM_116 | 592 | 1794 | virulence_ecoli | cea | Colicin E1 | 99.92 | 100.00 | CYCO01000082 |
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EHCKGBHM_83 | 220 | 1086 | amiA | 0.0 | N-acetylmuramoyl-L-alanine amidase | 100.00 | 100.00 | AAC75488 |
| EHCKGBHM_141 | 2056 | 3219 | senB | 0.0 | enterotoxin | 100.00 | 99.23 | ANZ22158 |
| EHCKGBHM_141 | 2 | 1984 | cjrC | 0.0 | TonB-dependent colicin J protein | 100.00 | 87.78 | ACU68762 |
| EHCKGBHM_64 | 1292 | 3 | QseC | 0.0 | sensory histidine kinase in two-component regulatory system | 99.07 | 95.77 | AAC76062 |
| EHCKGBHM_64 | 1292 | 3 | qseC | 0.0 | two-component regulatory system protein | 98.14 | 95.77 | AE005532_4 |
| EHCKGBHM_131 | 967 | 1944 | stbA | 0.0 | stability protein for the plasmid | 97.55 | 100.00 | AEE60096 |
| EHCKGBHM_25 | 1215 | 1733 | fecI | 2.76e-120 | RNA polymerase sigma factor | 100.00 | 100.00 | AAA23766 |
| EHCKGBHM_10 | 552 | 1 | satP | 3.42e-107 | succinate-acetate/proton symporter | 100.00 | 97.87 | AAC73121 |
| EHCKGBHM_132 | 1176 | 847 | pliG | 1.78e-73 | lysozyme inhibitor | 99.09 | 82.71 | NP_415696 |
| EHCKGBHM_125 | 154 | 1260 | vgrG2 | 0.0 | type VI secretion system Vgr family protein | 96.21 | 68.21 | AKK48148 |
| EHCKGBHM_88 | 1398 | 784 | FimH | 1.69e-140 | adhesion | 99.51 | 68.33 | CAA29156 |
| EHCKGBHM_88 | 1398 | 784 | fimH | 5.42e-119 | type 1 fimbriae adhesin | 82.93 | 67.88 | ABW24959 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| EHCKGBHM_16 | 671 | 932 | IncFII(pCoo) | 262 / 262 | 95.80 | CR942285 |
| EHCKGBHM_101 | 3707 | 3860 | Col156 | 154 / 154 | 94.81 | NC009781 |
| EHCKGBHM_61 | 749 | 1010 | Col(MG828) | 262 / 262 | 91.98 | NC008486 |
| EHCKGBHM_4 | 658 | 859 | Col8282 | 202 / 207 | 89.60 | DQ995352 |
| EHCKGBHM_129 | 3632 | 3763 | ColRNAI | 132 / 130 | 89.39 | DQ298019 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EHCKGBHM_1 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_2 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_3 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_4 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_5 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_6 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_7 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_8 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_9 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_10 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_11 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_12 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_13 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_14 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_15 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_16 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_17 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_18 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_19 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_20 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_21 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_22 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_23 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_24 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_25 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_26 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_27 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_28 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_29 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_30 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_31 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_32 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_33 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_34 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_35 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_36 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_37 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_38 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_39 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_40 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_41 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_42 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_43 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_44 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_45 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_46 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_47 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_48 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_49 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_50 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_51 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_52 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_53 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_54 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_55 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_56 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_57 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_58 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_59 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_60 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_61 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_62 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_63 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_64 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_65 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_66 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_67 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_68 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_69 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_70 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_71 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_72 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_73 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_74 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_75 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_76 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_77 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_78 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_79 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_80 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_81 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_82 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_83 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_84 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_85 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_86 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_87 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_88 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_89 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_90 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_91 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_92 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_93 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_94 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_95 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_96 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_97 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_98 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_99 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_100 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_101 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_102 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_103 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_104 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_105 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_106 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_107 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_108 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_109 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_110 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_111 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_112 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_113 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_114 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_115 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_116 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_117 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_118 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_119 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_120 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_121 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_122 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_123 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_124 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_125 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_126 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_127 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_128 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_129 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_130 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_131 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_132 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_133 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_134 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_135 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_136 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_137 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_138 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_139 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_140 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_141 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_142 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_143 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_144 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_145 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_146 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_147 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_148 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_149 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_150 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_151 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_152 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_153 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_154 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_155 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_156 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_157 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_158 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_159 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_160 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_161 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_162 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_163 | 1.0 | 0.0 | 1.0 |
| EHCKGBHM_164 | 0.0 | 1.0 | 0.0 |
| EHCKGBHM_165 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EHCKGBHM_20 | 55949 | 69015 | False | Unknown | VOG0572, |
| EHCKGBHM_41 | 30543 | 40071 | False | Unknown | VOG0298, |
Phigaro v2.4.0 (Database: Jan. 2024 release)