Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 3 | 3 | 6.56 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GLGOFGHD_108 | 53405 | 54370 | CfxA4 | ARO:3003005 | CfxA4 | 99.69 | 100.00 | AY769933.1 |
| GLGOFGHD_68 | 21130 | 23055 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
| GLGOFGHD_50 | 1741 | 2541 | ErmF | ARO:3000498 | ErmF | 98.50 | 100.00 | M17124.1 |
| GLGOFGHD_43 | 849 | 1583 | ErmG | ARO:3000522 | ErmG | 99.18 | 100.00 | L42817.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GLGOFGHD_108 | 53405 | 54370 | cfxA4 | Unknown Beta-lactam | 99.90 | 100.00 | AY769933 |
| GLGOFGHD_68 | 21130 | 23055 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.84 | 100.00 | L33696 |
| GLGOFGHD_50 | 1741 | 2541 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.50 | 100.00 | M17808 |
| GLGOFGHD_43 | 849 | 1583 | erm(G) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.32 | 100.00 | M15332 |
| GLGOFGHD_21 | 2857 | 3608 | cfxA6 | Unknown Beta-lactam | 86.45 | 75.40 | GQ342996 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GLGOFGHD_108 | 53408 | 54370 | cfxA4 | extended-spectrum class A beta-lactamase CfxA4 | BLASTX | 99.69 | 100.00 | WP_057280848.1 |
| GLGOFGHD_43 | 849 | 1580 | erm(G) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(G) | BLASTX | 99.59 | 100.00 | WP_063844791.1 |
| GLGOFGHD_68 | 21130 | 23052 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
| GLGOFGHD_50 | 1744 | 2541 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | BLASTX | 98.87 | 100.00 | WP_002682030.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GLGOFGHD_19 | 3311 | 6320 | agn43 | 0.0 | Antigen 43, AIDA-I type | Biofilm | 95.55 | 96.47 | YP_026164 |
| GLGOFGHD_26 | 2780 | 4345 | ECSMS35_RS25765 | 0.0 | Colicin E1 | Exotoxin | 95.21 | 99.94 | WP_000447001 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GLGOFGHD_26 | 3143 | 4345 | virulence_ecoli | cea | Colicin E1 | 99.92 | 100.00 | CYCO01000082 |
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GLGOFGHD_52 | 1589 | 2077 | yagT | 2.80e-107 | aldehyde dehydrogenase iron-sulfur subunit | 97.55 | 71.18 | AIZ85831 |
| GLGOFGHD_165 | 221 | 676 | tnpA | 1.68e-106 | regulatory RNA/ transposase for IS200 | 95.39 | 100.00 | AKH06467 |
| GLGOFGHD_66 | 778 | 846 | Z3917 | 3.99e-06 | 95.65 | 82.14 | AE005492_1 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| GLGOFGHD_10 | 178 | 857 | repUS2 | 682 / 681 | 98.09 | BFU30316 |
| GLGOFGHD_25 | 475 | 628 | Col156 | 154 / 154 | 94.81 | NC009781 |
| GLGOFGHD_26 | 1 | 109 | ColRNAI | 109 / 130 | 92.66 | DQ298019 |
| GLGOFGHD_103 | 1002 | 1263 | Col(MG828) | 262 / 262 | 91.98 | NC008486 |
| GLGOFGHD_28 | 415 | 616 | Col8282 | 202 / 207 | 89.60 | DQ995352 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GLGOFGHD_1 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_2 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_3 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_4 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_5 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_6 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_7 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_8 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_9 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_10 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_11 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_12 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_13 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_14 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_15 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_16 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_17 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_18 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_19 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_20 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_21 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_22 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_23 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_25 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_26 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_27 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_28 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_29 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_30 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_31 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_32 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_33 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_34 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_35 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_36 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_37 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_38 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_39 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_40 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_41 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_42 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_43 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_45 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_46 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_47 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_48 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_49 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_50 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_51 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_52 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_53 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_54 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_55 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_56 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_57 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_58 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_59 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_60 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_61 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_62 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_63 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_64 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_65 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_66 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_67 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_68 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_69 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_70 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_71 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_72 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_73 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_74 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_75 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_76 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_77 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_78 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_79 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_80 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_81 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_82 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_83 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_84 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_85 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_86 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_87 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_88 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_89 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_90 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_91 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_92 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_93 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_94 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_95 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_96 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_97 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_98 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_99 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_100 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_101 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_102 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_103 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_104 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_105 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_106 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_107 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_108 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_109 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_111 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_112 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_113 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_114 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_115 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_116 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_117 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_118 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_119 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_120 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_121 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_122 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_123 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_124 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_125 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_126 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_127 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_128 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_129 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_130 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_131 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_132 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_133 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_134 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_135 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_136 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_137 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_138 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_139 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_140 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_141 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_142 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_143 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_144 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_145 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_146 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_147 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_148 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_149 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_150 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_151 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_152 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_153 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_154 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_155 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_156 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_157 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_158 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_159 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_160 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_161 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_162 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_163 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_164 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_165 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_166 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_167 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_168 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_169 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_170 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_171 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_172 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_173 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_174 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_175 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_176 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_177 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_178 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_179 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_180 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_181 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_182 | 1.0 | 0.0 | 1.0 |
| GLGOFGHD_183 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_184 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_185 | 0.0 | 1.0 | 0.0 |
| GLGOFGHD_186 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GLGOFGHD_75 | 62346 | 70531 | False | Unknown | VOG0572, |
| GLGOFGHD_92 | 95955 | 108304 | False | Unknown | VOG0083, |
| GLGOFGHD_96 | 12949 | 21115 | False | Siphoviridae | VOG4972, |
Phigaro v2.4.0 (Database: Jan. 2024 release)