Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 3 | 2 | 6.16 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AKANMMGF_67 | 85499 | 86233 | ErmG | ARO:3000522 | ErmG | 99.18 | 100.00 | L42817.1 |
| AKANMMGF_100 | 34092 | 36065 | tet(Q) | ARO:3000191 | tet(Q) | 96.35 | 100.00 | Z21523.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| AKANMMGF_100 | 34092 | 36017 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.84 | 100.00 | L33696 |
| AKANMMGF_67 | 85499 | 86233 | erm(G) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.32 | 100.00 | M15332 |
| AKANMMGF_67 | 87005 | 88468 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 97.54 | 100.00 | AF274302 |
| AKANMMGF_67 | 88594 | 89808 | mef(A) | Erythromycin, Azithromycin | 94.24 | 99.75 | AF227520 |
| AKANMMGF_8 | 3332 | 4083 | cfxA6 | Unknown Beta-lactam | 86.45 | 75.40 | GQ342996 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AKANMMGF_67 | 85502 | 86233 | erm(G) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(G) | BLASTX | 99.59 | 100.00 | WP_063844791.1 |
| AKANMMGF_100 | 34095 | 36017 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
| AKANMMGF_67 | 87008 | 88468 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | BLASTX | 97.13 | 100.00 | WP_000420313.1 |
| AKANMMGF_67 | 88585 | 89808 | mef(A) | macrolide efflux MFS transporter Mef(A) | BLASTX | 96.57 | 100.00 | WP_012102963.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AKANMMGF_42 | 1 | 1153 | cka | 0.0 | Colicin K | Exotoxin | 99.83 | 70.01 | CAA61099 |
| AKANMMGF_15 | 2708 | 4273 | ECSMS35_RS25765 | 0.0 | Colicin E1 | Exotoxin | 95.21 | 99.94 | WP_000447001 |
| AKANMMGF_42 | 264 | 1153 | cfa | 0.0 | Col5 | Exotoxin | 88.58 | 60.22 | CAA61102 |
| AKANMMGF_42 | 264 | 1153 | cta | 0.0 | Col10 | Exotoxin | 80.87 | 60.15 | CAA57998 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AKANMMGF_15 | 3071 | 4273 | virulence_ecoli | cea | Colicin E1 | 99.92 | 100.00 | CYCO01000082 |
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AKANMMGF_43 | 144 | 1073 | PccS1_001441 | 0.0 | transcriptional regulator, LysR family, MetR | 84.19 | 99.68 | ACT15089 |
| AKANMMGF_43 | 144 | 1067 | metR | 2.21e-179 | HTH-type transcriptional regulator | 83.12 | 98.72 | CBA19149 |
| AKANMMGF_45 | 511 | 966 | tnpA | 1.12e-106 | regulatory RNA/ transposase for IS200 | 95.39 | 100.00 | AKH06467 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| AKANMMGF_41 | 147 | 826 | repUS2 | 682 / 681 | 98.53 | BFU30316 |
| AKANMMGF_13 | 3602 | 3755 | Col156 | 154 / 154 | 94.81 | NC009781 |
| AKANMMGF_32 | 11 | 272 | Col(MG828) | 262 / 262 | 91.60 | NC008486 |
| AKANMMGF_18 | 3559 | 3760 | Col8282 | 202 / 207 | 89.60 | DQ995352 |
| AKANMMGF_15 | 5060 | 5190 | ColRNAI | 131 / 130 | 87.02 | DQ298019 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AKANMMGF_1 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_2 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_3 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_4 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_5 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_6 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_7 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_8 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_9 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_10 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_11 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_12 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_13 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_14 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_15 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_16 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_17 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_18 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_19 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_20 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_21 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_22 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_24 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_25 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_26 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_27 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_28 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_29 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_30 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_31 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_32 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_33 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_34 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_35 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_37 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_38 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_39 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_40 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_41 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_42 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_43 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_44 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_45 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_46 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_47 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_48 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_49 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_50 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_51 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_52 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_53 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_54 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_55 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_56 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_57 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_58 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_59 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_60 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_61 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_62 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_63 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_64 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_65 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_66 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_67 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_68 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_69 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_70 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_71 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_72 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_73 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_74 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_75 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_76 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_77 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_78 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_79 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_80 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_81 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_82 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_83 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_84 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_85 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_86 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_87 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_88 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_89 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_90 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_91 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_92 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_93 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_94 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_95 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_96 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_97 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_98 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_99 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_100 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_101 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_102 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_103 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_104 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_105 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_106 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_107 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_108 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_109 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_110 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_111 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_112 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_113 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_114 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_115 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_116 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_117 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_118 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_119 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_120 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_121 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_122 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_123 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_124 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_125 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_126 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_127 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_128 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_129 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_130 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_131 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_132 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_133 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_134 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_135 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_136 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_137 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_138 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_139 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_140 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_141 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_142 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_143 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_144 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_145 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_146 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_147 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_148 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_149 | 0.0 | 1.0 | 0.0 |
| AKANMMGF_150 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_151 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_152 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_153 | 1.0 | 0.0 | 1.0 |
| AKANMMGF_154 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AKANMMGF_78 | 31712 | 42087 | False | Siphoviridae | VOG0749, |
| AKANMMGF_98 | 88807 | 96292 | False | Siphoviridae | VOG0204, |
Phigaro v2.4.0 (Database: Jan. 2024 release)