Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FLEMFGDH_1 | 2735465 | 2736319 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| FLEMFGDH_1 | 2718388 | 2719590 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| FLEMFGDH_1 | 2491893 | 2493062 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| FLEMFGDH_1 | 1358329 | 1358646 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| FLEMFGDH_1 | 1357991 | 1358329 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| FLEMFGDH_1 | 894956 | 897526 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| FLEMFGDH_1 | 591540 | 593183 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| FLEMFGDH_1 | 337310 | 337903 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| FLEMFGDH_1 | 286754 | 288187 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| FLEMFGDH_1 | 273938 | 274858 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FLEMFGDH_1 | 4198841 | 4200217 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | X08034 |
| FLEMFGDH_1 | 2735467 | 2736319 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| FLEMFGDH_1 | 273938 | 274858 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FLEMFGDH_1 | 4198844 | 4200217 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| FLEMFGDH_1 | 273938 | 274855 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| FLEMFGDH_1 | 591543 | 593183 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| FLEMFGDH_1 | 2735468 | 2736319 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| FLEMFGDH_1 | 4196324 | 4196842 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 89.60 | 100.00 | WP_003242546.1 |
| FLEMFGDH_1 | 2053303 | 2055900 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FLEMFGDH_1 | 2292725 | 2293366 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| FLEMFGDH_1 | 3714358 | 3715539 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| FLEMFGDH_1 | 3300074 | 3300859 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| FLEMFGDH_1 | 3296238 | 3297176 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391077 |
| FLEMFGDH_1 | 3298852 | 3300048 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| FLEMFGDH_1 | 2003222 | 2004985 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| FLEMFGDH_1 | 3289084 | 3296218 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.06 | 99.97 | NP_391076 |
| FLEMFGDH_1 | 3712733 | 3713875 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| FLEMFGDH_1 | 3713894 | 3714343 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| FLEMFGDH_1 | 3297204 | 3298823 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.52 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FLEMFGDH_1 | 132399 | 133535 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| FLEMFGDH_1 | 18665 | 19534 | pdxS | 3.14e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| FLEMFGDH_1 | 1668229 | 1669005 | CodY | 3.43e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FLEMFGDH_1 | 3553004 | 3553573 | ClpP | 2.54e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FLEMFGDH_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FLEMFGDH_1 | 1301438 | 1328503 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| FLEMFGDH_1 | 2171711 | 2176873 | False | Unknown | VOG4551, VOG0025, VOG7476, VOG0322 |
| FLEMFGDH_1 | 2238652 | 2250569 | False | Unknown | VOG9312, VOG4910, VOG3389, VOG5042 |
| FLEMFGDH_1 | 2669852 | 2699701 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796, VOG4673, VOG4693, VOG0189, VOG11060, VOG1309 |
Phigaro v2.4.0 (Database: Jan. 2024 release)