Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 14 | 10 | 3 | 17.46 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EGKLLJOG_1 | 2664537 | 2665391 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| EGKLLJOG_1 | 2646165 | 2647367 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| EGKLLJOG_1 | 2449262 | 2450431 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| EGKLLJOG_1 | 1462751 | 1463068 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| EGKLLJOG_1 | 1462413 | 1462751 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| EGKLLJOG_1 | 929471 | 932041 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| EGKLLJOG_1 | 592828 | 594471 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| EGKLLJOG_1 | 342403 | 342996 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| EGKLLJOG_1 | 330799 | 331449 | Streptococcus suis chloramphenicol acetyltransferase | ARO:3004455 | Ssui_ACT_CHL | 94.91 | 100.00 | AB080798.1 |
| EGKLLJOG_1 | 289560 | 290993 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| EGKLLJOG_1 | 276747 | 277667 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EGKLLJOG_1 | 330799 | 331449 | cat(pC194) | Chloramphenicol | 100.00 | 100.00 | NC_002013 |
| EGKLLJOG_1 | 4126419 | 4127795 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.91 | 100.00 | D12567 |
| EGKLLJOG_1 | 2664539 | 2665391 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| EGKLLJOG_1 | 276747 | 277667 | mph(K) | Spiramycin, Telithromycin | 98.48 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EGKLLJOG_1 | 330799 | 331446 | catA | type A-9 chloramphenicol O-acetyltransferase | EXACTX | 100.00 | 100.00 | WP_001010387.1 |
| EGKLLJOG_1 | 4126422 | 4127795 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_063856081.1 |
| EGKLLJOG_1 | 276747 | 277664 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| EGKLLJOG_1 | 592831 | 594471 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| EGKLLJOG_1 | 2664540 | 2665391 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| EGKLLJOG_1 | 4123948 | 4124466 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| EGKLLJOG_1 | 2141460 | 2144057 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.83 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EGKLLJOG_1 | 3216497 | 3217435 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| EGKLLJOG_1 | 3209341 | 3216477 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.78 | 100.00 | NP_391076 |
| EGKLLJOG_1 | 3635191 | 3636372 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| EGKLLJOG_1 | 3217463 | 3219082 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391078 |
| EGKLLJOG_1 | 3634727 | 3635176 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| EGKLLJOG_1 | 3220333 | 3221118 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| EGKLLJOG_1 | 2090930 | 2092693 | dep/capD | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | NP_389723 |
| EGKLLJOG_1 | 3219111 | 3220307 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| EGKLLJOG_1 | 2249316 | 2249957 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| EGKLLJOG_1 | 3633566 | 3634708 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EGKLLJOG_1 | 132337 | 133473 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| EGKLLJOG_1 | 18602 | 19471 | pdxS | 3.09e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EGKLLJOG_1 | 3475987 | 3476556 | ClpP | 2.50e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EGKLLJOG_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EGKLLJOG_1 | 640223 | 648419 | False | Siphoviridae | VOG0275, VOG4602, VOG3304, VOG9667, VOG0650, VOG4552, VOG4603, VOG8233 |
| EGKLLJOG_1 | 654826 | 682044 | False | Siphoviridae | VOG0198, VOG4681, VOG0796, VOG10227, VOG0720, VOG4564, VOG6778, VOG4555, VOG4553, VOG1125, VOG0815, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG2621, VOG0725, VOG4545, VOG4605, VOG4599, VOG5535, VOG10940, VOG0753, VOG0136, VOG4574, VOG1993, VOG2703, VOG9953 |
| EGKLLJOG_1 | 1320486 | 1346399 | False | Siphoviridae | VOG6430, VOG0531, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4626, VOG4705, VOG0753, VOG4574, VOG0602, VOG8440, VOG6116 |
| EGKLLJOG_1 | 1405679 | 1432602 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)