Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MPMHLFJC_1 | 2729445 | 2730299 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| MPMHLFJC_1 | 2710253 | 2711455 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| MPMHLFJC_1 | 2480921 | 2482090 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| MPMHLFJC_1 | 1348001 | 1348318 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MPMHLFJC_1 | 1347663 | 1348001 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MPMHLFJC_1 | 884907 | 887477 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.30 | 100.00 | AL009126.3 |
| MPMHLFJC_1 | 592627 | 594270 | vmlR | ARO:3004476 | vmlR | 98.90 | 100.00 | NC_000964.3 |
| MPMHLFJC_1 | 337314 | 337907 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| MPMHLFJC_1 | 286753 | 288186 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| MPMHLFJC_1 | 273938 | 274858 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MPMHLFJC_1 | 273938 | 274858 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| MPMHLFJC_1 | 4190016 | 4191392 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.77 | 100.00 | D12567 |
| MPMHLFJC_1 | 2729447 | 2730299 | aadK | Streptomycin | 98.12 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MPMHLFJC_1 | 592630 | 594270 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.90 | 100.00 | WP_003234144.1 |
| MPMHLFJC_1 | 273938 | 274855 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| MPMHLFJC_1 | 4190019 | 4191392 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| MPMHLFJC_1 | 2729448 | 2730299 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| MPMHLFJC_1 | 4187495 | 4188013 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| MPMHLFJC_1 | 2041358 | 2043955 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MPMHLFJC_1 | 3708594 | 3709775 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| MPMHLFJC_1 | 3283246 | 3290382 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391076 |
| MPMHLFJC_1 | 2281786 | 2282427 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| MPMHLFJC_1 | 3706969 | 3708111 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| MPMHLFJC_1 | 3294238 | 3295023 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| MPMHLFJC_1 | 3708130 | 3708579 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| MPMHLFJC_1 | 3290402 | 3291340 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| MPMHLFJC_1 | 3293016 | 3294212 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| MPMHLFJC_1 | 1991276 | 1993039 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| MPMHLFJC_1 | 3291368 | 3292987 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.07 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MPMHLFJC_1 | 132389 | 133525 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MPMHLFJC_1 | 18665 | 19534 | pdxS | 3.14e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MPMHLFJC_1 | 1657813 | 1658589 | CodY | 3.42e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MPMHLFJC_1 | 3547238 | 3547807 | ClpP | 2.54e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MPMHLFJC_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MPMHLFJC_1 | 1290607 | 1318179 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
| MPMHLFJC_1 | 2164173 | 2175095 | False | Siphoviridae | VOG0918, VOG4551, VOG0025, VOG7476, VOG0322, VOG6211, VOG0608, VOG4908 |
| MPMHLFJC_1 | 2229296 | 2253491 | False | Siphoviridae | VOG9219, VOG9713, VOG6940, VOG5534, VOG7718, VOG4705, VOG8610, VOG7930, VOG0275, VOG9667, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG7900, VOG4574, VOG8537 |
| MPMHLFJC_1 | 2658874 | 2677185 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749 |
Phigaro v2.4.0 (Database: Jan. 2024 release)