safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
OFADCEJM_1	2735268	2736122	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
OFADCEJM_1	2716559	2717761	blt	ARO:3003006	blt	100.00	100.00	L32599.1
OFADCEJM_1	2494240	2495409	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
OFADCEJM_1	1376445	1376762	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
OFADCEJM_1	1376107	1376445	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
OFADCEJM_1	916372	918942	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
OFADCEJM_1	604332	605975	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
OFADCEJM_1	338754	339347	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
OFADCEJM_1	288252	289685	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
OFADCEJM_1	275437	276357	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
OFADCEJM_1	2735268	2736122	aadK	Streptomycin	100.00	100.00	M26879
OFADCEJM_1	275437	276357	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
OFADCEJM_1	4187178	4188554	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
OFADCEJM_1	2735271	2736122	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
OFADCEJM_1	275437	276354	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
OFADCEJM_1	4184660	4185178	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
OFADCEJM_1	4187181	4188554	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
OFADCEJM_1	604335	605975	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
OFADCEJM_1	2050926	2053520	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
OFADCEJM_1	3697435	3698577	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
OFADCEJM_1	3699060	3700241	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
OFADCEJM_1	3698596	3699045	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
OFADCEJM_1	2004268	2006031	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
OFADCEJM_1	3291099	3291884	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
OFADCEJM_1	3287263	3288201	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
OFADCEJM_1	3289877	3291073	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
OFADCEJM_1	3288229	3289848	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
OFADCEJM_1	3280107	3287243	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
OFADCEJM_1	2294894	2295535	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
OFADCEJM_1	132473	133609	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
OFADCEJM_1	18665	19534	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
OFADCEJM_1	1689709	1690485	CodY	5.93e-124	nutrient-responsive regulator	80.69	100.00	ACJ80679
OFADCEJM_1	3545823	3546392	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
OFADCEJM_1	0.0	1.0	0.0
OFADCEJM_2	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
OFADCEJM_1	1320160	1346268	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
OFADCEJM_1	2666630	2696874	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600
OFADCEJM_2	12733	36381	False	Siphoviridae	VOG3616, VOG1024, VOG10944, VOG9244, VOG4599, VOG4605, VOG6163, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG0221
OFADCEJM_2	64436	74483	False	Unknown	VOG4549, VOG2725, VOG3851, VOG2352, VOG4714, VOG4561, VOG1198

Phigaro v2.4.0 (Database: Jan. 2024 release)