safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
IKJIFKGP_1	2583225	2584079	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
IKJIFKGP_1	2564516	2565718	blt	ARO:3003006	blt	100.00	100.00	L32599.1
IKJIFKGP_1	2339275	2340444	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
IKJIFKGP_1	1355883	1356200	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
IKJIFKGP_1	1355545	1355883	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
IKJIFKGP_1	895863	898433	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
IKJIFKGP_1	583823	585466	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
IKJIFKGP_1	338755	339348	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
IKJIFKGP_1	288252	289685	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
IKJIFKGP_1	275437	276357	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
IKJIFKGP_1	2583225	2584079	aadK	Streptomycin	100.00	100.00	M26879
IKJIFKGP_1	275437	276357	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
IKJIFKGP_1	4035134	4036510	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
IKJIFKGP_1	2583228	2584079	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
IKJIFKGP_1	275437	276354	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
IKJIFKGP_1	4032616	4033134	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
IKJIFKGP_1	4035137	4036510	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
IKJIFKGP_1	583826	585466	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
IKJIFKGP_1	2030363	2032957	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
IKJIFKGP_1	3545392	3546534	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
IKJIFKGP_1	3547017	3548198	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
IKJIFKGP_1	3546553	3547002	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
IKJIFKGP_1	1983705	1985468	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
IKJIFKGP_1	3139056	3139841	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
IKJIFKGP_1	3135220	3136158	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
IKJIFKGP_1	3137834	3139030	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
IKJIFKGP_1	3136186	3137805	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
IKJIFKGP_1	3128064	3135200	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
IKJIFKGP_1	2139929	2140570	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
IKJIFKGP_1	132473	133609	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
IKJIFKGP_1	18665	19534	pdxS	3.02e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
IKJIFKGP_1	1669146	1669922	CodY	3.30e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
IKJIFKGP_1	3393780	3394349	ClpP	2.44e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
IKJIFKGP_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
IKJIFKGP_1	1299598	1325706	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
IKJIFKGP_1	2511653	2530733	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG10095, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685

Phigaro v2.4.0 (Database: Jan. 2024 release)