Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AOJALFJL_1 | 3822616 | 3823536 | mphK | ARO:3004541 | mphK | 99.34 | 100.00 | CP010314.1 |
| AOJALFJL_1 | 3809290 | 3810723 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| AOJALFJL_1 | 3759610 | 3760203 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| AOJALFJL_1 | 3484545 | 3486191 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| AOJALFJL_1 | 3178299 | 3180869 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| AOJALFJL_1 | 2725628 | 2725966 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| AOJALFJL_1 | 2725311 | 2725628 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| AOJALFJL_1 | 1778625 | 1779794 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| AOJALFJL_1 | 1549349 | 1550551 | blt | ARO:3003006 | blt | 98.50 | 100.00 | L32599.1 |
| AOJALFJL_1 | 1531132 | 1531974 | aadK | ARO:3002627 | aadK | 97.83 | 98.59 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| AOJALFJL_1 | 3822616 | 3823536 | mph(K) | Spiramycin, Telithromycin | 99.24 | 100.00 | NC_000964 |
| AOJALFJL_1 | 1531111 | 1531963 | aadK | Streptomycin | 98.12 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AOJALFJL_1 | 3822628 | 3823536 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.34 | 99.02 | WP_003246254.1 |
| AOJALFJL_1 | 3484545 | 3486188 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| AOJALFJL_1 | 1531111 | 1531962 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| AOJALFJL_1 | 38161 | 38679 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| AOJALFJL_1 | 2068395 | 2070992 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AOJALFJL_1 | 2123517 | 2125280 | dep/capD | 0.0 | Capsule | Immune modulation | 99.94 | 100.00 | NP_389723 |
| AOJALFJL_1 | 522785 | 523966 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| AOJALFJL_1 | 1977184 | 1977825 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| AOJALFJL_1 | 945025 | 945810 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| AOJALFJL_1 | 945836 | 947032 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| AOJALFJL_1 | 948708 | 949646 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| AOJALFJL_1 | 524449 | 525591 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| AOJALFJL_1 | 523981 | 524430 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| AOJALFJL_1 | 949666 | 956802 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391076 |
| AOJALFJL_1 | 947061 | 948680 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.97 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AOJALFJL_1 | 3945177 | 3944041 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| AOJALFJL_1 | 4044984 | 4044115 | pdxS | 3.01e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| AOJALFJL_1 | 2414177 | 2413401 | CodY | 3.29e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| AOJALFJL_1 | 682394 | 681825 | ClpP | 2.44e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AOJALFJL_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AOJALFJL_1 | 1580923 | 1603327 | False | Myoviridae | VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4691, VOG4862 |
| AOJALFJL_1 | 2754387 | 2781367 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)