Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 23 | 33 | 40.27 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HNMCMCAK_9 | 33473 | 34936 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | AF274302 |
| HNMCMCAK_9 | 35056 | 36273 | mef(A) | Erythromycin, Azithromycin | 99.92 | 100.00 | U83667 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HNMCMCAK_9 | 35059 | 36273 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| HNMCMCAK_9 | 33476 | 34936 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | EXACTX | 100.00 | 100.00 | WP_000420313.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HNMCMCAK_7 | 73663 | 74514 | rfbD | 0.0 | Capsule | Immune modulation | 95.19 | 100.00 | WP_014621820 |
| HNMCMCAK_8 | 205190 | 205930 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 95.01 | 100.00 | WP_011681174 |
| HNMCMCAK_8 | 205190 | 205930 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 94.33 | 100.00 | WP_011226046 |
| HNMCMCAK_8 | 202287 | 203747 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 94.11 | 100.14 | WP_011226049 |
| HNMCMCAK_8 | 202287 | 203747 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.98 | 100.00 | WP_011681177 |
| HNMCMCAK_8 | 205987 | 207354 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.93 | 100.00 | WP_011226045 |
| HNMCMCAK_8 | 205190 | 205925 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 93.75 | 98.13 | WP_011227246 |
| HNMCMCAK_8 | 205987 | 207352 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 93.63 | 99.85 | WP_011681173 |
| HNMCMCAK_7 | 71291 | 72562 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 93.47 | 99.76 | WP_011226331 |
| HNMCMCAK_8 | 202287 | 203747 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.22 | 100.14 | WP_041827043 |
| HNMCMCAK_7 | 65715 | 66524 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 93.09 | 100.00 | WP_002948020 |
| HNMCMCAK_7 | 61674 | 63458 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.94 | 100.00 | WP_011226325 |
| HNMCMCAK_7 | 66508 | 67464 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 92.37 | 100.00 | WP_011226328 |
| HNMCMCAK_7 | 67461 | 68609 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 92.34 | 100.00 | WP_041828290 |
| HNMCMCAK_7 | 64510 | 65715 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.12 | 100.00 | WP_011226326 |
| HNMCMCAK_8 | 203748 | 204479 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.94 | 100.00 | WP_011226048 |
| HNMCMCAK_8 | 25980 | 26573 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.75 | 100.00 | WP_011226149 |
| HNMCMCAK_8 | 25980 | 26573 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.58 | 100.00 | WP_011226149 |
| HNMCMCAK_8 | 25980 | 26573 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.58 | 100.00 | WP_011226149 |
| HNMCMCAK_8 | 203748 | 204479 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.53 | 100.00 | WP_011681176 |
| HNMCMCAK_8 | 25111 | 25980 | rfbA | 0.0 | Capsule | Immune modulation | 91.26 | 100.00 | WP_011681286 |
| HNMCMCAK_7 | 63455 | 64489 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.10 | 99.15 | WP_002948022 |
| HNMCMCAK_8 | 203748 | 204479 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011227248 |
| HNMCMCAK_2 | 20615 | 21454 | cbpD | 0.0 | Autolysin | Exoenzyme | 90.96 | 99.88 | WP_011226733 |
| HNMCMCAK_8 | 315229 | 316887 | fbp54 | 0.0 | FBPs | Adherence | 90.48 | 99.94 | WP_011227179 |
| HNMCMCAK_1 | 23359 | 24369 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.45 | 99.80 | WP_002986042 |
| HNMCMCAK_8 | 204488 | 205180 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.04 | 99.86 | WP_011227247 |
| HNMCMCAK_8 | 204488 | 205180 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.04 | 99.86 | WP_011226047 |
| HNMCMCAK_8 | 204488 | 205180 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.46 | 99.86 | WP_011681175 |
| HNMCMCAK_9 | 55810 | 57113 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 87.25 | 99.54 | WP_000022832 |
| HNMCMCAK_13 | 114859 | 115620 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| HNMCMCAK_8 | 26845 | 27891 | rfbB | 0.0 | Capsule | Immune modulation | 80.92 | 99.90 | WP_009659423 |
| HNMCMCAK_10 | 213218 | 214501 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.61 | 99.61 | WP_000107753 |
| HNMCMCAK_8 | 215489 | 216074 | SPD_RS01775 | 7.25e-147 | Capsule | Immune modulation | 82.96 | 96.53 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HNMCMCAK_13 | 118308 | 119282 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.31 | 99.09 | AAK75326 |
| HNMCMCAK_13 | 106724 | 105651 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| HNMCMCAK_7 | 249131 | 251437 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.41 | 99.22 | AAK74619 |
| HNMCMCAK_9 | 144384 | 143416 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| HNMCMCAK_12 | 106588 | 105107 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| HNMCMCAK_13 | 87181 | 84935 | metE | 0.0 | methionine biosynthesis enzymes | 92.12 | 100.00 | AAK74738 |
| HNMCMCAK_2 | 35876 | 34905 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| HNMCMCAK_14 | 97987 | 99717 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| HNMCMCAK_7 | 86784 | 84796 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.59 | 100.00 | AE014259_2 |
| HNMCMCAK_7 | 115153 | 114002 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| HNMCMCAK_11 | 185221 | 183743 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| HNMCMCAK_9 | 57113 | 55812 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| HNMCMCAK_11 | 169848 | 167956 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| HNMCMCAK_13 | 82016 | 83725 | PGM | 0.0 | phosphoglucomutase | 85.11 | 99.65 | ACH87041 |
| HNMCMCAK_2 | 2037 | 946 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| HNMCMCAK_11 | 28537 | 29547 | pdh | 0.0 | pyruvate dehydrogenase | 83.38 | 99.70 | ABP91006 |
| HNMCMCAK_8 | 296656 | 298017 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| HNMCMCAK_9 | 71517 | 70627 | bglB | 1.86e-177 | putative phospho--glucosidase | 87.54 | 99.66 | AAK33487 |
| HNMCMCAK_13 | 42749 | 43699 | SP_0676 | 2.75e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| HNMCMCAK_13 | 84748 | 83891 | metF | 4.35e-169 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| HNMCMCAK_12 | 169144 | 169815 | rr01 | 3.49e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| HNMCMCAK_8 | 149228 | 149932 | covR | 1.52e-126 | responder protein | 85.53 | 100.00 | AAK71319 |
| HNMCMCAK_13 | 78049 | 77378 | ciaR | 1.10e-124 | two component system response regulator | 85.71 | 99.12 | AIK75708 |
| HNMCMCAK_13 | 78049 | 77381 | CiaR | 1.13e-124 | two-component response regulator | 87.00 | 99.55 | AAK74935 |
| HNMCMCAK_8 | 149228 | 149932 | walR | 3.46e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| HNMCMCAK_10 | 63346 | 62660 | CovR | 4.73e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| HNMCMCAK_2 | 772 | 377 | SpxA2 | 3.94e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| HNMCMCAK_8 | 285188 | 284787 | SpxA1 | 6.99e-70 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| HNMCMCAK_15 | 6604 | 6191 | GrpE | 3.80e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| HNMCMCAK_10 | 30655 | 30368 | spr0479 | 4.58e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| HNMCMCAK_9 | 178141 | 177887 | spr1327 | 1.48e-44 | essential gene | 92.94 | 100.00 | AAL00131 |
| HNMCMCAK_11 | 120669 | 120932 | spr0175 | 1.09e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| HNMCMCAK_11 | 121493 | 121759 | spr0177 | 2.42e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| HNMCMCAK_10 | 213051 | 212560 | RopE (M5005_Spy_1611) | 1.05e-72 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| HNMCMCAK_7 | 249131 | 251431 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.09 | 98.08 | MYN69560 |
| HNMCMCAK_11 | 64985 | 63966 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HNMCMCAK_1 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_2 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_3 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_4 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_5 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_6 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_7 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_8 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_9 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_10 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_11 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_12 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_13 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_14 | 0.0 | 1.0 | 0.0 |
| HNMCMCAK_15 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)