Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 34 | 41.05 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BNNIJHMO_22 | 184174 | 185025 | rfbD | 0.0 | Capsule | Immune modulation | 95.19 | 100.00 | WP_014621820 |
| BNNIJHMO_11 | 16567 | 18027 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 95.00 | 100.00 | WP_011681177 |
| BNNIJHMO_11 | 20267 | 21632 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.73 | 99.85 | WP_011681173 |
| BNNIJHMO_11 | 16567 | 18027 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 94.52 | 100.21 | WP_011226049 |
| BNNIJHMO_5 | 18960 | 20006 | rfbB | 0.0 | Capsule | Immune modulation | 94.27 | 100.00 | WP_002947383 |
| BNNIJHMO_11 | 20267 | 21634 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.08 | 100.00 | WP_011226045 |
| BNNIJHMO_11 | 19470 | 20210 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 94.06 | 100.00 | WP_011681174 |
| BNNIJHMO_11 | 18028 | 18759 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.99 | 100.00 | WP_011681176 |
| BNNIJHMO_11 | 18028 | 18759 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.85 | 100.00 | WP_011226048 |
| BNNIJHMO_11 | 16567 | 18027 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.84 | 100.21 | WP_041827043 |
| BNNIJHMO_22 | 186126 | 187397 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 93.47 | 99.76 | WP_011226331 |
| BNNIJHMO_11 | 18028 | 18759 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 93.17 | 100.00 | WP_011227248 |
| BNNIJHMO_11 | 19470 | 20210 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.12 | 100.00 | WP_011226046 |
| BNNIJHMO_22 | 192164 | 192973 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 93.09 | 100.00 | WP_002948020 |
| BNNIJHMO_22 | 195230 | 197014 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.94 | 100.00 | WP_011226325 |
| BNNIJHMO_5 | 20271 | 20864 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 92.93 | 100.00 | WP_011226149 |
| BNNIJHMO_11 | 19470 | 20205 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.80 | 98.13 | WP_011227246 |
| BNNIJHMO_5 | 20271 | 20864 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 92.76 | 100.00 | WP_011226149 |
| BNNIJHMO_5 | 20271 | 20864 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 92.76 | 100.00 | WP_011226149 |
| BNNIJHMO_22 | 191224 | 192180 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 92.37 | 100.00 | WP_011226328 |
| BNNIJHMO_22 | 190079 | 191227 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 92.34 | 100.00 | WP_041828290 |
| BNNIJHMO_22 | 192973 | 194178 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.12 | 100.00 | WP_011226326 |
| BNNIJHMO_11 | 124354 | 126011 | fbp54 | 0.0 | FBPs | Adherence | 91.20 | 99.88 | WP_011227179 |
| BNNIJHMO_22 | 194199 | 195233 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.10 | 99.15 | WP_002948022 |
| BNNIJHMO_5 | 20864 | 21733 | rfbA | 0.0 | Capsule | Immune modulation | 91.03 | 100.00 | WP_011681286 |
| BNNIJHMO_25 | 131 | 1141 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.55 | 99.80 | WP_002986042 |
| BNNIJHMO_11 | 18768 | 19460 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.33 | 99.86 | WP_011227247 |
| BNNIJHMO_11 | 18768 | 19460 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.33 | 99.86 | WP_011226047 |
| BNNIJHMO_14 | 21832 | 22671 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.99 | 99.88 | WP_011226733 |
| BNNIJHMO_11 | 18768 | 19460 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.46 | 99.86 | WP_011681175 |
| BNNIJHMO_33 | 150496 | 151799 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 87.25 | 99.54 | WP_000022832 |
| BNNIJHMO_12 | 22301 | 23062 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| BNNIJHMO_24 | 1900 | 3183 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.68 | 99.61 | WP_000107753 |
| BNNIJHMO_11 | 29785 | 30368 | SPD_RS01775 | 1.33e-157 | Capsule | Immune modulation | 84.10 | 96.20 | WP_001819836 |
| BNNIJHMO_48 | 1 | 1147 | cka | 0.0 | Colicin K | Exotoxin | 99.65 | 69.64 | CAA61099 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BNNIJHMO_12 | 25751 | 26734 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.65 | 100.00 | AAK75326 |
| BNNIJHMO_12 | 14168 | 13095 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| BNNIJHMO_22 | 2405 | 99 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.15 | 99.22 | AAK74619 |
| BNNIJHMO_52 | 34483 | 35964 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| BNNIJHMO_33 | 64637 | 65605 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| BNNIJHMO_29 | 3412 | 1166 | metE | 0.0 | methionine biosynthesis enzymes | 92.26 | 100.00 | AAK74738 |
| BNNIJHMO_17 | 1971 | 2942 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| BNNIJHMO_3 | 7046 | 483 | PrtA | 0.0 | serine protease | 90.05 | 101.96 | ABJ55268 |
| BNNIJHMO_21 | 9596 | 11326 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.78 | 100.00 | AAK34199 |
| BNNIJHMO_22 | 171722 | 173710 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.74 | 100.00 | AE014259_2 |
| BNNIJHMO_22 | 142981 | 144132 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| BNNIJHMO_43 | 207889 | 206411 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| BNNIJHMO_33 | 150496 | 151797 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| BNNIJHMO_14 | 1886 | 807 | recA | 0.0 | DNA recombination and repair | 85.56 | 94.07 | AAL00560 |
| BNNIJHMO_43 | 192560 | 190668 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.26 | 98.90 | ABP91129 |
| BNNIJHMO_11 | 225698 | 227407 | PGM | 0.0 | phosphoglucomutase | 85.11 | 99.65 | ACH87041 |
| BNNIJHMO_4 | 28752 | 29762 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| BNNIJHMO_11 | 108793 | 110154 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.46 | 99.56 | ABP90420 |
| BNNIJHMO_33 | 136090 | 136980 | bglB | 1.07e-177 | putative phospho--glucosidase | 87.54 | 99.66 | AAK33487 |
| BNNIJHMO_11 | 186591 | 187541 | SP_0676 | 5.38e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| BNNIJHMO_29 | 979 | 122 | metF | 1.59e-169 | methionine biosynthesis enzymes | 87.41 | 99.31 | AAK74739 |
| BNNIJHMO_3 | 25165 | 24494 | rr01 | 1.05e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| BNNIJHMO_10 | 454 | 1158 | covR | 1.18e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| BNNIJHMO_11 | 221729 | 221058 | ciaR | 1.02e-124 | two component system response regulator | 85.71 | 99.12 | AIK75708 |
| BNNIJHMO_11 | 221729 | 221061 | CiaR | 1.26e-124 | two-component response regulator | 87.00 | 99.55 | AAK74935 |
| BNNIJHMO_10 | 454 | 1158 | walR | 2.69e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| BNNIJHMO_56 | 64543 | 65229 | CovR | 1.67e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| BNNIJHMO_14 | 631 | 236 | SpxA2 | 2.23e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| BNNIJHMO_11 | 97326 | 96925 | SpxA1 | 4.66e-70 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| BNNIJHMO_2 | 6825 | 6412 | GrpE | 5.54e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| BNNIJHMO_13 | 11259 | 11546 | spr0479 | 6.48e-49 | essential gene | 85.42 | 98.97 | AAK99283 |
| BNNIJHMO_33 | 28525 | 28779 | spr1327 | 9.59e-45 | essential gene | 92.94 | 100.00 | AAL00131 |
| BNNIJHMO_43 | 161401 | 161664 | spr0175 | 1.26e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| BNNIJHMO_43 | 162227 | 162493 | spr0177 | 1.75e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| BNNIJHMO_24 | 3350 | 3841 | RopE (M5005_Spy_1611) | 5.55e-74 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| BNNIJHMO_22 | 2405 | 105 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.83 | 98.08 | MYN69560 |
| BNNIJHMO_43 | 105638 | 104619 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| BNNIJHMO_45 | 1002 | 1263 | Col(MG828) | 262 / 262 | 91.98 | NC008486 |
| BNNIJHMO_44 | 917 | 1048 | ColRNAI | 132 / 130 | 89.39 | DQ298019 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BNNIJHMO_1 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_2 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_3 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_4 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_5 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_6 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_7 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_8 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_9 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_10 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_11 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_12 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_13 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_14 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_15 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_16 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_17 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_18 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_19 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_21 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_22 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_23 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_24 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_25 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_26 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_27 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_28 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_29 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_30 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_31 | 1.0 | 0.0 | 1.0 |
| BNNIJHMO_32 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_33 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_34 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_37 | 1.0 | 0.0 | 1.0 |
| BNNIJHMO_38 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_39 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_41 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_42 | 1.0 | 0.0 | 1.0 |
| BNNIJHMO_43 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_44 | 1.0 | 0.0 | 1.0 |
| BNNIJHMO_45 | 1.0 | 0.0 | 1.0 |
| BNNIJHMO_47 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_48 | 1.0 | 0.0 | 1.0 |
| BNNIJHMO_49 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_51 | 1.0 | 0.0 | 1.0 |
| BNNIJHMO_52 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_53 | 1.0 | 0.0 | 1.0 |
| BNNIJHMO_54 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_55 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_56 | 0.0 | 1.0 | 0.0 |
| BNNIJHMO_57 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)