Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 16 | 34 | 37.70 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IJMKIBFA_33 | 39119 | 40597 | lsaC | ARO:3003112 | lsaC | 99.39 | 100.00 | HM990671.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IJMKIBFA_42 | 108989 | 110206 | mef(A) | Erythromycin, Azithromycin | 99.92 | 100.00 | HG423652 |
| IJMKIBFA_33 | 39119 | 40597 | lsa(C) | Lincomycin, Clindamycin, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 99.80 | 100.00 | HM990671 |
| IJMKIBFA_42 | 110332 | 111795 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 98.50 | 100.00 | AF227520 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IJMKIBFA_33 | 39122 | 40597 | lsa(C) | ABC-F type ribosomal protection protein Lsa(C) | BLASTX | 99.39 | 100.00 | WP_063851347.1 |
| IJMKIBFA_42 | 108989 | 110203 | mef(A) | macrolide efflux MFS transporter Mef(A) | BLASTX | 98.02 | 100.00 | WP_063853563.1 |
| IJMKIBFA_42 | 110332 | 111792 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | BLASTX | 97.74 | 100.00 | WP_000420317.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IJMKIBFA_3 | 7880 | 9340 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.06 | 100.00 | WP_011681177 |
| IJMKIBFA_3 | 7880 | 9340 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.58 | 100.21 | WP_011226049 |
| IJMKIBFA_3 | 7880 | 9340 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.03 | 100.21 | WP_041827043 |
| IJMKIBFA_3 | 11580 | 12945 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.73 | 99.85 | WP_011681173 |
| IJMKIBFA_3 | 11580 | 12947 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.08 | 100.00 | WP_011226045 |
| IJMKIBFA_3 | 10783 | 11523 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 94.06 | 100.00 | WP_011681174 |
| IJMKIBFA_3 | 9341 | 10072 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.99 | 100.00 | WP_011681176 |
| IJMKIBFA_3 | 9341 | 10072 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.85 | 100.00 | WP_011226048 |
| IJMKIBFA_3 | 9341 | 10072 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 93.17 | 100.00 | WP_011227248 |
| IJMKIBFA_3 | 10783 | 11523 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.12 | 100.00 | WP_011226046 |
| IJMKIBFA_11 | 96939 | 97789 | rfbD | 0.0 | Capsule | Immune modulation | 92.95 | 99.88 | WP_014621820 |
| IJMKIBFA_3 | 10783 | 11518 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.80 | 98.13 | WP_011227246 |
| IJMKIBFA_45 | 79069 | 79662 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 90.74 | 100.00 | WP_011226149 |
| IJMKIBFA_45 | 79662 | 80531 | rfbA | 0.0 | Capsule | Immune modulation | 90.58 | 100.00 | WP_011681286 |
| IJMKIBFA_45 | 79069 | 79662 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.57 | 100.00 | WP_011226149 |
| IJMKIBFA_45 | 79069 | 79662 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.57 | 100.00 | WP_011226149 |
| IJMKIBFA_6 | 26523 | 28181 | fbp54 | 0.0 | FBPs | Adherence | 89.94 | 99.94 | WP_011227179 |
| IJMKIBFA_17 | 131 | 1141 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.55 | 99.80 | WP_002986042 |
| IJMKIBFA_3 | 10081 | 10773 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.33 | 99.86 | WP_011227247 |
| IJMKIBFA_3 | 10081 | 10773 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.33 | 99.86 | WP_011226047 |
| IJMKIBFA_13 | 22010 | 22849 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.99 | 99.88 | WP_011226733 |
| IJMKIBFA_3 | 10081 | 10773 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.46 | 99.86 | WP_011681175 |
| IJMKIBFA_42 | 87630 | 88933 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 87.33 | 99.54 | WP_000022832 |
| IJMKIBFA_9 | 4500 | 5261 | srtA | 0.0 | Sortase A | Exoenzyme | 85.06 | 99.87 | - |
| IJMKIBFA_26 | 2667 | 3950 | tig/ropA | 0.0 | Trigger factor | Stress survival | 81.07 | 99.61 | WP_000107753 |
| IJMKIBFA_45 | 77755 | 78801 | rfbB | 0.0 | Capsule | Immune modulation | 80.52 | 100.00 | WP_009659423 |
| IJMKIBFA_3 | 21000 | 21583 | SPD_RS01775 | 3.73e-158 | Capsule | Immune modulation | 84.10 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IJMKIBFA_9 | 1811 | 828 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.65 | 100.00 | AAK75326 |
| IJMKIBFA_9 | 13400 | 14473 | MntE | 0.0 | manganese homeostasis | 95.25 | 99.72 | ABF32167 |
| IJMKIBFA_11 | 275447 | 277753 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.15 | 99.22 | AAK74619 |
| IJMKIBFA_22 | 108924 | 110405 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| IJMKIBFA_9 | 32466 | 34712 | metE | 0.0 | methionine biosynthesis enzymes | 92.12 | 100.00 | AAK74738 |
| IJMKIBFA_25 | 2049 | 1081 | spr0084 | 0.0 | pathogenitcity related | 91.95 | 98.48 | AAK98888 |
| IJMKIBFA_18 | 234 | 1205 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| IJMKIBFA_19 | 3500 | 1770 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.78 | 100.00 | AAK34199 |
| IJMKIBFA_11 | 139950 | 138799 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| IJMKIBFA_11 | 111229 | 109241 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.29 | 100.00 | AE014259_2 |
| IJMKIBFA_33 | 135520 | 134042 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| IJMKIBFA_42 | 87630 | 88931 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| IJMKIBFA_33 | 120188 | 118296 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.26 | 98.90 | ABP91129 |
| IJMKIBFA_2 | 65354 | 67063 | PGM | 0.0 | phosphoglucomutase | 85.11 | 99.65 | ACH87041 |
| IJMKIBFA_13 | 2043 | 952 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| IJMKIBFA_8 | 28492 | 29502 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| IJMKIBFA_6 | 53008 | 51647 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| IJMKIBFA_42 | 73223 | 74113 | bglB | 1.83e-178 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| IJMKIBFA_2 | 26107 | 27057 | SP_0676 | 1.99e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| IJMKIBFA_9 | 34899 | 35756 | metF | 6.86e-169 | methionine biosynthesis enzymes | 87.41 | 99.31 | AAK74739 |
| IJMKIBFA_11 | 97789 | 96950 | GacA | 4.68e-150 | dTDP-4-dehydrorhamnose reductas | 80.00 | 98.59 | AAZ51220 |
| IJMKIBFA_47 | 47930 | 48634 | covR | 3.30e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| IJMKIBFA_22 | 60528 | 59857 | rr01 | 3.47e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| IJMKIBFA_2 | 61385 | 60714 | ciaR | 2.99e-125 | two component system response regulator | 85.71 | 99.12 | AIK75708 |
| IJMKIBFA_2 | 61385 | 60717 | CiaR | 3.72e-125 | two-component response regulator | 87.00 | 99.55 | AAK74935 |
| IJMKIBFA_47 | 47930 | 48634 | walR | 7.50e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| IJMKIBFA_44 | 49214 | 48528 | CovR | 2.48e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| IJMKIBFA_13 | 776 | 381 | SpxA2 | 2.25e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| IJMKIBFA_12 | 24664 | 24263 | SpxA1 | 3.42e-70 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| IJMKIBFA_5 | 55490 | 55903 | GrpE | 9.74e-68 | protein grpE | 81.16 | 80.59 | ABP89267 |
| IJMKIBFA_44 | 26009 | 25722 | spr0479 | 3.01e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| IJMKIBFA_1 | 30188 | 30442 | spr1327 | 3.12e-45 | essential gene | 92.94 | 100.00 | AAL00131 |
| IJMKIBFA_33 | 89029 | 89292 | spr0175 | 8.34e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| IJMKIBFA_33 | 89855 | 90121 | spr0177 | 1.16e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| IJMKIBFA_26 | 2500 | 2009 | RopE (M5005_Spy_1611) | 3.72e-74 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| IJMKIBFA_11 | 275447 | 277747 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.83 | 98.08 | MYN69560 |
| IJMKIBFA_33 | 1852 | 833 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IJMKIBFA_1 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_2 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_3 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_4 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_5 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_6 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_7 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_8 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_9 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_10 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_11 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_12 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_13 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_14 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_15 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_16 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_17 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_18 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_19 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_20 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_21 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_22 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_23 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_24 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_25 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_26 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_27 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_29 | 1.0 | 0.0 | 1.0 |
| IJMKIBFA_30 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_31 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_33 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_34 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_35 | 1.0 | 0.0 | 1.0 |
| IJMKIBFA_37 | 1.0 | 0.0 | 1.0 |
| IJMKIBFA_38 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_39 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_40 | 1.0 | 0.0 | 1.0 |
| IJMKIBFA_41 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_42 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_43 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_44 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_45 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_46 | 0.0 | 1.0 | 0.0 |
| IJMKIBFA_47 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)