Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 34 | 41.05 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KPKKBODK_11 | 183971 | 184822 | rfbD | 0.0 | Capsule | Immune modulation | 95.19 | 100.00 | WP_014621820 |
| KPKKBODK_45 | 85759 | 87219 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 95.00 | 100.00 | WP_011681177 |
| KPKKBODK_45 | 82154 | 83519 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.73 | 99.85 | WP_011681173 |
| KPKKBODK_45 | 85759 | 87219 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 94.52 | 100.21 | WP_011226049 |
| KPKKBODK_6 | 18960 | 20006 | rfbB | 0.0 | Capsule | Immune modulation | 94.27 | 100.00 | WP_002947383 |
| KPKKBODK_45 | 82152 | 83519 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.08 | 100.00 | WP_011226045 |
| KPKKBODK_45 | 83576 | 84316 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 94.06 | 100.00 | WP_011681174 |
| KPKKBODK_45 | 85027 | 85758 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.99 | 100.00 | WP_011681176 |
| KPKKBODK_45 | 85027 | 85758 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.85 | 100.00 | WP_011226048 |
| KPKKBODK_45 | 85759 | 87219 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.84 | 100.21 | WP_041827043 |
| KPKKBODK_11 | 185923 | 187194 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 93.47 | 99.76 | WP_011226331 |
| KPKKBODK_45 | 85027 | 85758 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 93.17 | 100.00 | WP_011227248 |
| KPKKBODK_45 | 83576 | 84316 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.12 | 100.00 | WP_011226046 |
| KPKKBODK_11 | 191961 | 192770 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 93.09 | 100.00 | WP_002948020 |
| KPKKBODK_11 | 195027 | 196811 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.94 | 100.00 | WP_011226325 |
| KPKKBODK_6 | 20271 | 20864 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 92.93 | 100.00 | WP_011226149 |
| KPKKBODK_45 | 83581 | 84316 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.80 | 98.13 | WP_011227246 |
| KPKKBODK_6 | 20271 | 20864 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 92.76 | 100.00 | WP_011226149 |
| KPKKBODK_6 | 20271 | 20864 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 92.76 | 100.00 | WP_011226149 |
| KPKKBODK_11 | 191021 | 191977 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 92.37 | 100.00 | WP_011226328 |
| KPKKBODK_11 | 189876 | 191024 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 92.34 | 100.00 | WP_041828290 |
| KPKKBODK_11 | 192770 | 193975 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.12 | 100.00 | WP_011226326 |
| KPKKBODK_47 | 73479 | 75136 | fbp54 | 0.0 | FBPs | Adherence | 91.20 | 99.88 | WP_011227179 |
| KPKKBODK_11 | 193996 | 195030 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.10 | 99.15 | WP_002948022 |
| KPKKBODK_6 | 20864 | 21733 | rfbA | 0.0 | Capsule | Immune modulation | 91.03 | 100.00 | WP_011681286 |
| KPKKBODK_24 | 15801 | 16811 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.55 | 99.80 | WP_002986042 |
| KPKKBODK_45 | 84326 | 85018 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.33 | 99.86 | WP_011227247 |
| KPKKBODK_45 | 84326 | 85018 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.33 | 99.86 | WP_011226047 |
| KPKKBODK_14 | 140 | 979 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.99 | 99.88 | WP_011226733 |
| KPKKBODK_45 | 84326 | 85018 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.46 | 99.86 | WP_011681175 |
| KPKKBODK_22 | 184872 | 186175 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 87.25 | 99.54 | WP_000022832 |
| KPKKBODK_12 | 22301 | 23062 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| KPKKBODK_21 | 1900 | 3183 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.68 | 99.61 | WP_000107753 |
| KPKKBODK_45 | 73418 | 74001 | SPD_RS01775 | 6.05e-158 | Capsule | Immune modulation | 84.10 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KPKKBODK_12 | 25751 | 26734 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.65 | 100.00 | AAK75326 |
| KPKKBODK_12 | 14168 | 13095 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| KPKKBODK_11 | 2405 | 99 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.15 | 99.22 | AAK74619 |
| KPKKBODK_43 | 77849 | 76368 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| KPKKBODK_22 | 99013 | 99981 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| KPKKBODK_27 | 3412 | 1166 | metE | 0.0 | methionine biosynthesis enzymes | 92.26 | 100.00 | AAK74738 |
| KPKKBODK_17 | 15359 | 14388 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| KPKKBODK_4 | 7046 | 483 | PrtA | 0.0 | serine protease | 90.05 | 101.96 | ABJ55268 |
| KPKKBODK_19 | 9596 | 11326 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.78 | 100.00 | AAK34199 |
| KPKKBODK_11 | 171701 | 173689 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.59 | 100.00 | AE014259_2 |
| KPKKBODK_11 | 142979 | 144130 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| KPKKBODK_33 | 208994 | 207516 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| KPKKBODK_22 | 184872 | 186173 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| KPKKBODK_14 | 20925 | 22004 | recA | 0.0 | DNA recombination and repair | 85.56 | 94.07 | AAL00560 |
| KPKKBODK_33 | 193665 | 191773 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.26 | 98.90 | ABP91129 |
| KPKKBODK_9 | 26321 | 28030 | PGM | 0.0 | phosphoglucomutase | 85.11 | 99.65 | ACH87041 |
| KPKKBODK_5 | 28878 | 29888 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| KPKKBODK_47 | 90697 | 89336 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.46 | 99.56 | ABP90420 |
| KPKKBODK_22 | 170466 | 171356 | bglB | 1.25e-177 | putative phospho--glucosidase | 87.54 | 99.66 | AAK33487 |
| KPKKBODK_47 | 12899 | 11949 | SP_0676 | 2.25e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| KPKKBODK_27 | 979 | 122 | metF | 1.59e-169 | methionine biosynthesis enzymes | 87.41 | 99.31 | AAK74739 |
| KPKKBODK_4 | 25165 | 24494 | rr01 | 1.05e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| KPKKBODK_10 | 454 | 1158 | covR | 1.18e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| KPKKBODK_9 | 22352 | 21681 | ciaR | 1.25e-125 | two component system response regulator | 85.71 | 99.12 | AIK75708 |
| KPKKBODK_9 | 22352 | 21684 | CiaR | 1.55e-125 | two-component response regulator | 87.00 | 99.55 | AAK74935 |
| KPKKBODK_10 | 454 | 1158 | walR | 2.69e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| KPKKBODK_49 | 65313 | 65999 | CovR | 1.69e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| KPKKBODK_14 | 22180 | 22575 | SpxA2 | 2.23e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| KPKKBODK_45 | 6460 | 6861 | SpxA1 | 2.12e-70 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| KPKKBODK_3 | 53907 | 53494 | GrpE | 9.20e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| KPKKBODK_13 | 11259 | 11546 | spr0479 | 6.48e-49 | essential gene | 85.42 | 98.97 | AAK99283 |
| KPKKBODK_22 | 62901 | 63155 | spr1327 | 1.12e-44 | essential gene | 92.94 | 100.00 | AAL00131 |
| KPKKBODK_33 | 162506 | 162769 | spr0175 | 1.21e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| KPKKBODK_33 | 163332 | 163598 | spr0177 | 1.69e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| KPKKBODK_21 | 3350 | 3841 | RopE (M5005_Spy_1611) | 5.55e-74 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| KPKKBODK_11 | 2405 | 105 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.83 | 98.08 | MYN69560 |
| KPKKBODK_33 | 106743 | 105724 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KPKKBODK_1 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_2 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_3 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_4 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_5 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_6 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_7 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_8 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_9 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_10 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_11 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_12 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_13 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_14 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_15 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_16 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_17 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_18 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_19 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_20 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_21 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_22 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_23 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_24 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_25 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_26 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_27 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_28 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_29 | 1.0 | 0.0 | 1.0 |
| KPKKBODK_31 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_33 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_34 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_35 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_36 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_37 | 1.0 | 0.0 | 1.0 |
| KPKKBODK_38 | 1.0 | 0.0 | 1.0 |
| KPKKBODK_39 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_40 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_41 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_42 | 1.0 | 0.0 | 1.0 |
| KPKKBODK_43 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_44 | 1.0 | 0.0 | 1.0 |
| KPKKBODK_45 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_46 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_47 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_48 | 0.0 | 1.0 | 0.0 |
| KPKKBODK_49 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)