safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	7	17.83

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
BHKKDACK_1	1639295	1640149	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
BHKKDACK_1	1620586	1621788	blt	ARO:3003006	blt	100.00	100.00	L32599.1
BHKKDACK_1	1398281	1399450	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
BHKKDACK_1	425646	425963	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
BHKKDACK_1	425308	425646	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
BHKKDACK_4	391573	393216	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
BHKKDACK_4	162965	163558	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
BHKKDACK_4	112462	113901	lmrB	ARO:3002813	lmrB	99.79	100.42	JYFL01000006.1
BHKKDACK_4	99647	100567	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
BHKKDACK_5	27372	29942	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
BHKKDACK_1	1639295	1640149	aadK	Streptomycin	100.00	100.00	M26879
BHKKDACK_4	99647	100567	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
BHKKDACK_3	36393	37769	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
BHKKDACK_1	1639298	1640149	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
BHKKDACK_4	99647	100564	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
BHKKDACK_3	39769	40287	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
BHKKDACK_3	36393	37766	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
BHKKDACK_4	391576	393216	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
BHKKDACK_1	1089350	1091944	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
BHKKDACK_3	526115	527257	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
BHKKDACK_3	524451	525632	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
BHKKDACK_3	525647	526096	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
BHKKDACK_1	1042692	1044455	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
BHKKDACK_3	932781	933566	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
BHKKDACK_3	936464	937402	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
BHKKDACK_3	933592	934788	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
BHKKDACK_3	934817	936436	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
BHKKDACK_3	937422	944558	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
BHKKDACK_1	1198915	1199556	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BHKKDACK_6	28134	26998	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
BHKKDACK_8	11305	10436	pdxS	1.12e-151	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
BHKKDACK_1	738917	739693	CodY	1.69e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
BHKKDACK_3	678843	678274	ClpP	6.29e-99	part of proteolytic complex	81.05	97.44	BAB94595
BHKKDACK_1	276485	276877	spxA1	4.99e-69	redox-responsive transcription factor	83.21	100.00	AAF21893
BHKKDACK_7	20621	20863	SpoVG	3.91e-38	RNA-binding protein	80.25	79.41	AAT02994
BHKKDACK_4	346101	346298	CspA	1.82e-31	cold shock protein	90.91	100.00	CAA62903

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BHKKDACK_1	0.0	1.0	0.0
BHKKDACK_3	0.0	1.0	0.0
BHKKDACK_4	0.0	1.0	0.0
BHKKDACK_5	0.0	1.0	0.0
BHKKDACK_6	0.0	1.0	0.0
BHKKDACK_7	0.0	1.0	0.0
BHKKDACK_8	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
BHKKDACK_1	369361	395469	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
BHKKDACK_1	1570658	1600902	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)