safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
11	10	9	17.38

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
BEJHNKKA_84	100413	101582	bmr	ARO:3003007	bmr	99.49	100.00	M33768.1
BEJHNKKA_139	63921	64841	mphK	ARO:3004541	mphK	96.73	100.00	CP010314.1
BEJHNKKA_139	50590	52029	lmrB	ARO:3002813	lmrB	99.58	100.42	JYFL01000006.1
BEJHNKKA_67	36700	37554	aadK	ARO:3002627	aadK	99.30	100.00	AL009126.1
BEJHNKKA_150	27382	29952	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	99.65	100.00	AL009126.3
BEJHNKKA_11	23486	23824	ykkC	ARO:3003063	ykkC	98.21	100.00	AL009126.1
BEJHNKKA_11	23169	23486	ykkD	ARO:3003064	ykkD	98.10	100.00	AL009126.1
BEJHNKKA_22	19950	21587	vmlR	ARO:3004476	vmlR	98.72	99.63	NC_000964.3
BEJHNKKA_67	7411	8613	blt	ARO:3003006	blt	99.25	100.00	L32599.1
BEJHNKKA_139	901	1494	tmrB	ARO:3003059	tmrB	97.97	100.00	AL009126.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
BEJHNKKA_67	36700	37554	aadK	Streptomycin	99.18	100.00	M26879
BEJHNKKA_139	63921	64841	mph(K)	Spiramycin, Telithromycin	96.96	100.00	NC_000964

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
BEJHNKKA_67	36703	37554	aadK	aminoglycoside 6-adenylyltransferase AadK	BLASTX	99.30	100.00	WP_003229862.1
BEJHNKKA_22	19950	21590	vmlR	ABC-F type ribosomal protection protein VmlR	INTERNAL_STOP	98.54	99.82	WP_003234144.1
BEJHNKKA_139	63924	64841	mphK	macrolide 2'-phosphotransferase MphK	BLASTX	96.73	100.00	WP_003246254.1
BEJHNKKA_120	8778	11375	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	80.83	100.00	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
BEJHNKKA_33	57829	58614	dhbA	Bacillibactin	Nutritional/Metabolic factor	99.75	100.00	NP_391080
BEJHNKKA_81	31004	31453	capC	Capsule	Immune modulation	99.56	100.00	NP_391470
BEJHNKKA_6	4644	5285	hlyIII	Hemolysin III	Exotoxin	99.53	100.00	NP_390062
BEJHNKKA_81	31468	32649	capB	Capsule	Immune modulation	99.49	100.00	NP_391471
BEJHNKKA_81	29843	30985	capA	Capsule	Immune modulation	99.21	100.00	NP_391469
BEJHNKKA_33	56607	57803	dhbC	Bacillibactin	Nutritional/Metabolic factor	98.91	100.00	NP_391079
BEJHNKKA_123	3695	5458	dep/capD	Capsule	Immune modulation	98.70	100.00	NP_389723
BEJHNKKA_33	46837	53973	dhbF	Bacillibactin	Nutritional/Metabolic factor	98.64	100.00	NP_391076
BEJHNKKA_33	54959	56578	dhbE	Bacillibactin	Nutritional/Metabolic factor	98.46	100.00	NP_391078
BEJHNKKA_33	53993	54931	dhbB	Bacillibactin	Nutritional/Metabolic factor	98.30	100.00	NP_391077

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BEJHNKKA_86	28071	26935	tufA	0.0	elongation factor Tu	85.75	95.69	AHM69299
BEJHNKKA_119	4328	5197	pdxS	1.11e-151	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
BEJHNKKA_95	70638	71414	CodY	9.68e-126	nutrient-responsive regulator	81.08	100.00	ACJ80679
BEJHNKKA_117	1031	462	ClpP	7.04e-100	part of proteolytic complex	81.05	97.44	BAB94595
BEJHNKKA_15	3333	2941	spxA1	1.55e-72	redox-responsive transcription factor	83.97	100.00	AAF21893
BEJHNKKA_15	3333	2941	spx	9.91e-71	essential gene which is a thiol/oxidative stress sensor	80.15	100.00	BAB57159
BEJHNKKA_91	8146	7904	SpoVG	3.90e-39	RNA-binding protein	81.48	79.41	AAT02994
BEJHNKKA_22	75408	75211	CspA	3.78e-32	cold shock protein	90.91	100.00	CAA62903
BEJHNKKA_88	23955	23761	CspD	1.93e-29	cold shock protein	81.54	98.48	CAC99957
BEJHNKKA_68	38731	38925	CspB	4.46e-28	cold shock protein	83.08	98.48	CAD00094

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BEJHNKKA_1	0.0	1.0	0.0
BEJHNKKA_2	0.0	1.0	0.0
BEJHNKKA_3	0.0	1.0	0.0
BEJHNKKA_4	0.0	1.0	0.0
BEJHNKKA_5	0.0	1.0	0.0
BEJHNKKA_6	0.0	1.0	0.0
BEJHNKKA_7	0.0	1.0	0.0
BEJHNKKA_8	0.0	1.0	0.0
BEJHNKKA_9	0.0	1.0	0.0
BEJHNKKA_10	0.0	1.0	0.0
BEJHNKKA_11	0.0	1.0	0.0
BEJHNKKA_12	0.0	1.0	0.0
BEJHNKKA_13	0.0	1.0	0.0
BEJHNKKA_14	0.0	1.0	0.0
BEJHNKKA_15	0.0	1.0	0.0
BEJHNKKA_16	0.0	1.0	0.0
BEJHNKKA_17	0.0	1.0	0.0
BEJHNKKA_18	0.0	1.0	0.0
BEJHNKKA_19	0.0	1.0	0.0
BEJHNKKA_20	0.0	1.0	0.0
BEJHNKKA_22	0.0	1.0	0.0
BEJHNKKA_24	0.0	1.0	0.0
BEJHNKKA_25	0.0	1.0	0.0
BEJHNKKA_26	0.0	1.0	0.0
BEJHNKKA_33	0.0	1.0	0.0
BEJHNKKA_44	0.0	1.0	0.0
BEJHNKKA_55	0.0	1.0	0.0
BEJHNKKA_57	0.0	1.0	0.0
BEJHNKKA_58	0.0	1.0	0.0
BEJHNKKA_59	0.0	1.0	0.0
BEJHNKKA_60	0.0	1.0	0.0
BEJHNKKA_61	0.0	1.0	0.0
BEJHNKKA_62	0.0	1.0	0.0
BEJHNKKA_63	0.0	1.0	0.0
BEJHNKKA_64	0.0	1.0	0.0
BEJHNKKA_65	0.0	1.0	0.0
BEJHNKKA_66	0.0	1.0	0.0
BEJHNKKA_67	0.0	1.0	0.0
BEJHNKKA_68	0.0	1.0	0.0
BEJHNKKA_69	0.0	1.0	0.0
BEJHNKKA_70	0.0	1.0	0.0
BEJHNKKA_71	0.0	1.0	0.0
BEJHNKKA_72	0.0	1.0	0.0
BEJHNKKA_73	0.0	1.0	0.0
BEJHNKKA_74	0.0	1.0	0.0
BEJHNKKA_75	0.0	1.0	0.0
BEJHNKKA_76	0.0	1.0	0.0
BEJHNKKA_77	0.0	1.0	0.0
BEJHNKKA_78	0.0	1.0	0.0
BEJHNKKA_79	0.0	1.0	0.0
BEJHNKKA_80	0.0	1.0	0.0
BEJHNKKA_81	0.0	1.0	0.0
BEJHNKKA_82	0.0	1.0	0.0
BEJHNKKA_83	0.0	1.0	0.0
BEJHNKKA_84	0.0	1.0	0.0
BEJHNKKA_85	0.0	1.0	0.0
BEJHNKKA_86	0.0	1.0	0.0
BEJHNKKA_87	0.0	1.0	0.0
BEJHNKKA_88	0.0	1.0	0.0
BEJHNKKA_89	0.0	1.0	0.0
BEJHNKKA_90	0.0	1.0	0.0
BEJHNKKA_91	0.0	1.0	0.0
BEJHNKKA_92	0.0	1.0	0.0
BEJHNKKA_93	0.0	1.0	0.0
BEJHNKKA_94	0.0	1.0	0.0
BEJHNKKA_95	0.0	1.0	0.0
BEJHNKKA_96	0.0	1.0	0.0
BEJHNKKA_97	0.0	1.0	0.0
BEJHNKKA_98	0.0	1.0	0.0
BEJHNKKA_99	0.0	1.0	0.0
BEJHNKKA_100	0.0	1.0	0.0
BEJHNKKA_101	0.0	1.0	0.0
BEJHNKKA_102	0.0	1.0	0.0
BEJHNKKA_103	0.0	1.0	0.0
BEJHNKKA_104	0.0	1.0	0.0
BEJHNKKA_105	0.0	1.0	0.0
BEJHNKKA_106	0.0	1.0	0.0
BEJHNKKA_107	0.0	1.0	0.0
BEJHNKKA_108	1.0	0.0	1.0
BEJHNKKA_109	0.0	1.0	0.0
BEJHNKKA_110	0.0	1.0	0.0
BEJHNKKA_111	0.0	1.0	0.0
BEJHNKKA_112	0.0	1.0	0.0
BEJHNKKA_113	0.0	1.0	0.0
BEJHNKKA_114	0.0	1.0	0.0
BEJHNKKA_115	0.0	1.0	0.0
BEJHNKKA_116	0.0	1.0	0.0
BEJHNKKA_117	0.0	1.0	0.0
BEJHNKKA_118	0.0	1.0	0.0
BEJHNKKA_119	0.0	1.0	0.0
BEJHNKKA_120	0.0	1.0	0.0
BEJHNKKA_121	0.0	1.0	0.0
BEJHNKKA_122	0.0	1.0	0.0
BEJHNKKA_123	0.0	1.0	0.0
BEJHNKKA_124	0.0	1.0	0.0
BEJHNKKA_125	0.0	1.0	0.0
BEJHNKKA_126	0.0	1.0	0.0
BEJHNKKA_127	0.0	1.0	0.0
BEJHNKKA_128	0.0	1.0	0.0
BEJHNKKA_129	0.0	1.0	0.0
BEJHNKKA_130	0.0	1.0	0.0
BEJHNKKA_131	0.0	1.0	0.0
BEJHNKKA_132	0.0	1.0	0.0
BEJHNKKA_133	0.0	1.0	0.0
BEJHNKKA_134	0.0	1.0	0.0
BEJHNKKA_135	0.0	1.0	0.0
BEJHNKKA_136	0.0	1.0	0.0
BEJHNKKA_137	0.0	1.0	0.0
BEJHNKKA_138	0.0	1.0	0.0
BEJHNKKA_139	0.0	1.0	0.0
BEJHNKKA_140	0.0	1.0	0.0
BEJHNKKA_141	0.0	1.0	0.0
BEJHNKKA_142	0.0	1.0	0.0
BEJHNKKA_143	0.0	1.0	0.0
BEJHNKKA_144	0.0	1.0	0.0
BEJHNKKA_145	0.0	1.0	0.0
BEJHNKKA_146	0.0	1.0	0.0
BEJHNKKA_147	0.0	1.0	0.0
BEJHNKKA_148	0.0	1.0	0.0
BEJHNKKA_149	0.0	1.0	0.0
BEJHNKKA_150	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
BEJHNKKA_11	53694	81264	False	Myoviridae	VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667