Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 3 | 15.91 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DNGPGFBA_1 | 2664280 | 2665482 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| DNGPGFBA_1 | 2491023 | 2492192 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DNGPGFBA_1 | 1424682 | 1424999 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DNGPGFBA_1 | 1424344 | 1424682 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DNGPGFBA_1 | 963394 | 965964 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| DNGPGFBA_1 | 642585 | 644231 | vmlR | ARO:3004476 | vmlR | 98.91 | 100.18 | NC_000964.3 |
| DNGPGFBA_1 | 372083 | 372676 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| DNGPGFBA_1 | 321587 | 323020 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| DNGPGFBA_1 | 308774 | 309694 | mphK | ARO:3004541 | mphK | 99.67 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DNGPGFBA_1 | 308774 | 309694 | mph(K) | Spiramycin, Telithromycin | 99.57 | 100.00 | NC_000964 |
| DNGPGFBA_1 | 4178562 | 4179938 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.84 | 100.00 | D12567 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DNGPGFBA_1 | 308774 | 309691 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.67 | 100.00 | WP_003246254.1 |
| DNGPGFBA_1 | 4178565 | 4179938 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_063856081.1 |
| DNGPGFBA_1 | 642588 | 644231 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.91 | 100.00 | WP_003234144.1 |
| DNGPGFBA_1 | 4176076 | 4176594 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| DNGPGFBA_1 | 2008415 | 2011003 | rphC | rifamycin-inactivating phosphotransferase RphC | INTERNAL_STOP | 80.25 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DNGPGFBA_1 | 3259418 | 3260203 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| DNGPGFBA_1 | 3686588 | 3687037 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| DNGPGFBA_1 | 3687052 | 3688233 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| DNGPGFBA_1 | 2252450 | 2253091 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| DNGPGFBA_1 | 3685427 | 3686569 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| DNGPGFBA_1 | 3255582 | 3256520 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| DNGPGFBA_1 | 1962659 | 1964422 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| DNGPGFBA_1 | 3248426 | 3255562 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.70 | 100.00 | NP_391076 |
| DNGPGFBA_1 | 3258196 | 3259392 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| DNGPGFBA_1 | 3256548 | 3258167 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.53 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DNGPGFBA_1 | 133494 | 134630 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DNGPGFBA_1 | 1733444 | 1734220 | CodY | 3.41e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DNGPGFBA_1 | 3534637 | 3535206 | ClpP | 2.53e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DNGPGFBA_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DNGPGFBA_1 | 165200 | 189395 | False | Siphoviridae | VOG2752, VOG10041, VOG7372, VOG0198, VOG8909, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG3390, VOG4705, VOG0641, VOG4707 |
| DNGPGFBA_1 | 1369817 | 1395113 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG7895, VOG0753 |
| DNGPGFBA_1 | 2209609 | 2217514 | False | Siphoviridae | VOG3389, VOG5042, VOG9712, VOG4626, VOG3390, VOG4705 |
| DNGPGFBA_1 | 2284723 | 2301302 | False | Siphoviridae | VOG4779, VOG9219, VOG4713, VOG4572, VOG4555, VOG0720, VOG7734, VOG0221, VOG3699, VOG0673, VOG8610, VOG4705 |
| DNGPGFBA_1 | 3121844 | 3144491 | False | Myoviridae | VOG4574, VOG0641, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG1350, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555, VOG0720 |
| DNGPGFBA_1 | 3152188 | 3155761 | False | Unknown | VOG0189, VOG4566, VOG1309, VOG4600 |
Phigaro v2.4.0 (Database: Jan. 2024 release)