Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GDPPEOOA_1 | 2361987 | 2362841 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| GDPPEOOA_1 | 2345169 | 2346371 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| GDPPEOOA_1 | 2173931 | 2175100 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| GDPPEOOA_1 | 1165736 | 1166053 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| GDPPEOOA_1 | 1165398 | 1165736 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| GDPPEOOA_1 | 704348 | 706918 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| GDPPEOOA_1 | 397893 | 399518 | vmlR | ARO:3004476 | vmlR | 98.71 | 98.90 | NC_000964.3 |
| GDPPEOOA_1 | 144204 | 144797 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| GDPPEOOA_1 | 93701 | 95134 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| GDPPEOOA_1 | 80881 | 81801 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GDPPEOOA_1 | 3811330 | 3812706 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.35 | 100.00 | D12567 |
| GDPPEOOA_1 | 2361987 | 2362841 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| GDPPEOOA_1 | 80881 | 81801 | mph(K) | Spiramycin, Telithromycin | 97.94 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GDPPEOOA_1 | 3811333 | 3812706 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_063856081.1 |
| GDPPEOOA_1 | 397896 | 399518 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.71 | 98.90 | WP_003234144.1 |
| GDPPEOOA_1 | 2361990 | 2362841 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| GDPPEOOA_1 | 80881 | 81798 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| GDPPEOOA_1 | 3808953 | 3809471 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.22 | 100.00 | WP_003242546.1 |
| GDPPEOOA_1 | 1846799 | 1849396 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.37 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GDPPEOOA_1 | 2916775 | 2917560 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.87 | 100.00 | NP_391080 |
| GDPPEOOA_1 | 3334047 | 3335228 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| GDPPEOOA_1 | 3333583 | 3334032 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| GDPPEOOA_1 | 1945361 | 1946002 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| GDPPEOOA_1 | 1799877 | 1801640 | dep/capD | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | NP_389723 |
| GDPPEOOA_1 | 3332422 | 3333564 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| GDPPEOOA_1 | 2915553 | 2916749 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| GDPPEOOA_1 | 2905783 | 2912919 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.03 | 100.00 | NP_391076 |
| GDPPEOOA_1 | 2912939 | 2913877 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391077 |
| GDPPEOOA_1 | 2913905 | 2915524 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.79 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GDPPEOOA_1 | 3972131 | 3973267 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| GDPPEOOA_1 | 3858322 | 3859191 | pdxS | 2.99e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| GDPPEOOA_1 | 1476305 | 1477081 | CodY | 3.27e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GDPPEOOA_1 | 3172675 | 3173244 | ClpP | 2.42e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GDPPEOOA_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GDPPEOOA_1 | 1108785 | 1135909 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)