Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ABCHOCKG_1 | 2732401 | 2733321 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
| ABCHOCKG_1 | 2719075 | 2720508 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| ABCHOCKG_1 | 2669353 | 2669946 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| ABCHOCKG_1 | 2416407 | 2418050 | vmlR | ARO:3004476 | vmlR | 96.71 | 100.00 | NC_000964.3 |
| ABCHOCKG_1 | 2095558 | 2098128 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| ABCHOCKG_1 | 1630353 | 1630691 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| ABCHOCKG_1 | 1630036 | 1630353 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| ABCHOCKG_1 | 488731 | 489900 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| ABCHOCKG_1 | 230404 | 231606 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| ABCHOCKG_1 | 213481 | 214335 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ABCHOCKG_1 | 213481 | 214333 | aadK | Streptomycin | 98.83 | 99.77 | M26879 |
| ABCHOCKG_1 | 2732401 | 2733321 | mph(K) | Spiramycin, Telithromycin | 98.15 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ABCHOCKG_1 | 213481 | 214332 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| ABCHOCKG_1 | 2732404 | 2733321 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| ABCHOCKG_1 | 2416407 | 2418047 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.71 | 100.00 | WP_003234144.1 |
| ABCHOCKG_1 | 3032856 | 3033374 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
| ABCHOCKG_1 | 910445 | 913042 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.83 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ABCHOCKG_1 | 3923383 | 3924168 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.87 | 100.00 | NP_391080 |
| ABCHOCKG_1 | 3507664 | 3508845 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| ABCHOCKG_1 | 3927066 | 3928004 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| ABCHOCKG_1 | 3509328 | 3510470 | capA | 0.0 | Capsule | Immune modulation | 99.12 | 100.00 | NP_391469 |
| ABCHOCKG_1 | 3508860 | 3509309 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| ABCHOCKG_1 | 3924194 | 3925390 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| ABCHOCKG_1 | 960683 | 962446 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| ABCHOCKG_1 | 686577 | 687218 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| ABCHOCKG_1 | 3928024 | 3935160 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.47 | 100.00 | NP_391076 |
| ABCHOCKG_1 | 3925419 | 3927038 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.35 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ABCHOCKG_1 | 2866370 | 2865234 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| ABCHOCKG_1 | 2980108 | 2979239 | pdxS | 1.23e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| ABCHOCKG_1 | 1318495 | 1317719 | CodY | 3.47e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ABCHOCKG_1 | 3671551 | 3670982 | ClpP | 2.58e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ABCHOCKG_1 | 0.0 | 1.0 | 0.0 |
| ABCHOCKG_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ABCHOCKG_1 | 287408 | 310204 | False | Myoviridae | VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| ABCHOCKG_1 | 730315 | 745917 | False | Unknown | VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG7930, VOG8610, VOG4705 |
| ABCHOCKG_1 | 798463 | 804018 | False | Unknown | VOG6211, VOG0322, VOG7476, VOG0025 |
| ABCHOCKG_1 | 1093032 | 1115314 | False | Siphoviridae | VOG0641, VOG7896, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4573, VOG4556, VOG2935, VOG1886, VOG4581, VOG4841 |
| ABCHOCKG_1 | 1122389 | 1127501 | False | Siphoviridae | VOG9600, VOG4566, VOG4552, VOG0181, VOG5353, VOG9667 |
| ABCHOCKG_1 | 1660539 | 1688201 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)