Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EJCAPAJL_1 | 3858142 | 3860712 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| EJCAPAJL_1 | 3401350 | 3401688 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| EJCAPAJL_1 | 3401033 | 3401350 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| EJCAPAJL_1 | 2369670 | 2370839 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| EJCAPAJL_1 | 2176459 | 2177661 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| EJCAPAJL_1 | 2158082 | 2158936 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| EJCAPAJL_1 | 388170 | 389090 | mphK | ARO:3004541 | mphK | 97.39 | 100.00 | CP010314.1 |
| EJCAPAJL_1 | 374844 | 376277 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| EJCAPAJL_1 | 325159 | 325752 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| EJCAPAJL_1 | 53308 | 54951 | vmlR | ARO:3004476 | vmlR | 98.17 | 100.00 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EJCAPAJL_1 | 694173 | 695549 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.78 | 100.00 | D12567 |
| EJCAPAJL_1 | 2158082 | 2158936 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| EJCAPAJL_1 | 388170 | 389090 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EJCAPAJL_1 | 694173 | 695546 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_063856081.1 |
| EJCAPAJL_1 | 2158082 | 2158933 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| EJCAPAJL_1 | 53308 | 54948 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.17 | 100.00 | WP_003234144.1 |
| EJCAPAJL_1 | 388173 | 389090 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.39 | 100.00 | WP_003246254.1 |
| EJCAPAJL_1 | 697524 | 698042 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| EJCAPAJL_1 | 2674057 | 2676654 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EJCAPAJL_1 | 1605836 | 1606774 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| EJCAPAJL_1 | 2572991 | 2573632 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| EJCAPAJL_1 | 1604189 | 1605808 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.81 | 100.00 | NP_391078 |
| EJCAPAJL_1 | 1606794 | 1613930 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391076 |
| EJCAPAJL_1 | 1186609 | 1187790 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| EJCAPAJL_1 | 1187805 | 1188254 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| EJCAPAJL_1 | 2726725 | 2728488 | dep/capD | 0.0 | Capsule | Immune modulation | 99.55 | 100.00 | NP_389723 |
| EJCAPAJL_1 | 1602964 | 1604160 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.42 | 100.00 | NP_391079 |
| EJCAPAJL_1 | 1602153 | 1602938 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| EJCAPAJL_1 | 1188273 | 1189415 | capA | 0.0 | Capsule | Immune modulation | 99.12 | 100.00 | NP_391469 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EJCAPAJL_1 | 531119 | 529983 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| EJCAPAJL_1 | 644846 | 643977 | pdxS | 3.05e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EJCAPAJL_1 | 3058718 | 3057942 | CodY | 3.33e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EJCAPAJL_1 | 1349661 | 1349092 | ClpP | 2.47e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EJCAPAJL_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EJCAPAJL_1 | 3432646 | 3458349 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490 |
Phigaro v2.4.0 (Database: Jan. 2024 release)