Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OFJPNBIK_1 | 2454703 | 2455623 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| OFJPNBIK_1 | 2441377 | 2442810 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| OFJPNBIK_1 | 2391696 | 2392289 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| OFJPNBIK_1 | 2148676 | 2150319 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| OFJPNBIK_1 | 1849458 | 1852028 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| OFJPNBIK_1 | 1389983 | 1390321 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OFJPNBIK_1 | 1389666 | 1389983 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| OFJPNBIK_1 | 277211 | 278380 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| OFJPNBIK_1 | 54896 | 56098 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| OFJPNBIK_1 | 36511 | 37365 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OFJPNBIK_1 | 2454703 | 2455623 | mph(K) | Spiramycin, Telithromycin | 99.89 | 100.00 | NC_000964 |
| OFJPNBIK_1 | 2755926 | 2757302 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.06 | 100.00 | D12567 |
| OFJPNBIK_1 | 36511 | 37363 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OFJPNBIK_1 | 2454706 | 2455623 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| OFJPNBIK_1 | 2755926 | 2757299 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_063856081.1 |
| OFJPNBIK_1 | 2148676 | 2150316 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| OFJPNBIK_1 | 36511 | 37362 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| OFJPNBIK_1 | 2759336 | 2759854 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| OFJPNBIK_1 | 714060 | 716654 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OFJPNBIK_1 | 3686274 | 3687212 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| OFJPNBIK_1 | 3236594 | 3237775 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| OFJPNBIK_1 | 3682591 | 3683376 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| OFJPNBIK_1 | 3238258 | 3239400 | capA | 0.0 | Capsule | Immune modulation | 99.47 | 100.00 | NP_391469 |
| OFJPNBIK_1 | 477106 | 477747 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| OFJPNBIK_1 | 3237790 | 3238239 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| OFJPNBIK_1 | 760316 | 762079 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| OFJPNBIK_1 | 3683402 | 3684598 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| OFJPNBIK_1 | 3687232 | 3694368 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.98 | 100.00 | NP_391076 |
| OFJPNBIK_1 | 3684627 | 3686246 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.89 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OFJPNBIK_1 | 2593553 | 2592417 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| OFJPNBIK_1 | 2707360 | 2706491 | pdxS | 3.13e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| OFJPNBIK_1 | 1076640 | 1075864 | CodY | 3.42e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OFJPNBIK_1 | 3390997 | 3390428 | ClpP | 2.53e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OFJPNBIK_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OFJPNBIK_1 | 75655 | 105683 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4691, VOG4862 |
| OFJPNBIK_1 | 499590 | 519636 | False | Unknown | VOG10230, VOG8440, VOG5070, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG7930, VOG8610, VOG7895 |
| OFJPNBIK_1 | 578686 | 583848 | False | Unknown | VOG6211, VOG0322, VOG7476, VOG0025 |
| OFJPNBIK_1 | 1420160 | 1446268 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| OFJPNBIK_1 | 3486593 | 3490290 | False | Siphoviridae | VOG0275, VOG1563, VOG9667 |
| OFJPNBIK_1 | 3494788 | 3521447 | False | Siphoviridae | VOG4694, VOG4567, VOG0198, VOG0275, VOG0531, VOG4841, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641, VOG4707 |
Phigaro v2.4.0 (Database: Jan. 2024 release)