Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MAAABMCE_1 | 3426918 | 3427838 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
| MAAABMCE_1 | 3413591 | 3415024 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.00 | JYFL01000006.1 |
| MAAABMCE_1 | 3363875 | 3364468 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| MAAABMCE_1 | 3105668 | 3107314 | vmlR | ARO:3004476 | vmlR | 99.45 | 100.18 | NC_000964.3 |
| MAAABMCE_1 | 2793461 | 2796031 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| MAAABMCE_1 | 2283437 | 2283775 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MAAABMCE_1 | 2283120 | 2283437 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MAAABMCE_1 | 1172126 | 1173295 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MAAABMCE_1 | 998842 | 1000044 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| MAAABMCE_1 | 982141 | 982974 | aadK | ARO:3002627 | aadK | 97.11 | 97.54 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MAAABMCE_1 | 3426918 | 3427838 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| MAAABMCE_1 | 3713715 | 3715091 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.13 | 100.00 | X08034 |
| MAAABMCE_1 | 982120 | 982974 | aadK | Streptomycin | 98.25 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MAAABMCE_1 | 3105668 | 3107311 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.45 | 100.00 | WP_003234144.1 |
| MAAABMCE_1 | 3713715 | 3715088 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| MAAABMCE_1 | 3426921 | 3427838 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| MAAABMCE_1 | 982120 | 982971 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.18 | 100.00 | WP_003229862.1 |
| MAAABMCE_1 | 3717059 | 3717577 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| MAAABMCE_1 | 1596096 | 1598693 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.76 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MAAABMCE_1 | 1369955 | 1370596 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| MAAABMCE_1 | 14263 | 15444 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| MAAABMCE_1 | 433452 | 434237 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| MAAABMCE_1 | 1651162 | 1652925 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| MAAABMCE_1 | 437135 | 438073 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| MAAABMCE_1 | 15927 | 17069 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| MAAABMCE_1 | 15459 | 15908 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| MAAABMCE_1 | 438093 | 445229 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391076 |
| MAAABMCE_1 | 434263 | 435459 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.50 | 100.00 | NP_391079 |
| MAAABMCE_1 | 435488 | 437107 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.16 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MAAABMCE_1 | 3556151 | 3555015 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MAAABMCE_1 | 3669965 | 3669096 | pdxS | 3.11e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MAAABMCE_1 | 1971980 | 1971204 | CodY | 3.40e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MAAABMCE_1 | 172016 | 171447 | ClpP | 2.52e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MAAABMCE_1 | 0.0 | 1.0 | 0.0 |
| MAAABMCE_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MAAABMCE_1 | 1400558 | 1417868 | False | Siphoviridae | VOG4705, VOG3390, VOG4626, VOG9712, VOG5042, VOG3389, VOG4910, VOG6163, VOG9312, VOG9312 |
| MAAABMCE_1 | 1426469 | 1436396 | False | Myoviridae | VOG7134, VOG10940, VOG5721, VOG5779, VOG5722, VOG4570 |
| MAAABMCE_1 | 2313014 | 2338313 | False | Myoviridae | VOG0136, VOG0753, VOG7895, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| MAAABMCE_1 | 2403249 | 2424974 | False | Siphoviridae | VOG5535, VOG4599, VOG4605, VOG4545, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG0815, VOG1125, VOG4553, VOG4555, VOG6778, VOG4564, VOG0720, VOG10227, VOG0796 |
Phigaro v2.4.0 (Database: Jan. 2024 release)