Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FJGKIOOH_1 | 3652142 | 3652480 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| FJGKIOOH_1 | 3651825 | 3652142 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| FJGKIOOH_1 | 2644714 | 2645883 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| FJGKIOOH_1 | 2450318 | 2451520 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| FJGKIOOH_1 | 2431853 | 2432707 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| FJGKIOOH_1 | 669419 | 670339 | mphK | ARO:3004541 | mphK | 96.70 | 100.00 | CP010314.1 |
| FJGKIOOH_1 | 656090 | 657523 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| FJGKIOOH_1 | 606436 | 607029 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| FJGKIOOH_1 | 357241 | 358884 | vmlR | ARO:3004476 | vmlR | 96.71 | 100.00 | NC_000964.3 |
| FJGKIOOH_1 | 61740 | 64310 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FJGKIOOH_1 | 982252 | 983628 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.42 | 100.00 | D12567 |
| FJGKIOOH_1 | 2431853 | 2432705 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| FJGKIOOH_1 | 669419 | 670339 | mph(K) | Spiramycin, Telithromycin | 97.29 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FJGKIOOH_1 | 982252 | 983625 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_063856081.1 |
| FJGKIOOH_1 | 2431853 | 2432704 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| FJGKIOOH_1 | 357241 | 358881 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.71 | 100.00 | WP_003234144.1 |
| FJGKIOOH_1 | 669431 | 670339 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.70 | 99.02 | WP_003246254.1 |
| FJGKIOOH_1 | 985641 | 986159 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| FJGKIOOH_1 | 2956395 | 2958989 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.26 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FJGKIOOH_1 | 2848089 | 2848730 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| FJGKIOOH_1 | 1472303 | 1473484 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| FJGKIOOH_1 | 1874286 | 1875071 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| FJGKIOOH_1 | 1473967 | 1475109 | capA | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | NP_391469 |
| FJGKIOOH_1 | 3007320 | 3009083 | dep/capD | 0.0 | Capsule | Immune modulation | 99.43 | 100.00 | NP_389723 |
| FJGKIOOH_1 | 1473499 | 1473948 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| FJGKIOOH_1 | 1875097 | 1876293 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| FJGKIOOH_1 | 1878927 | 1886063 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.74 | 100.00 | NP_391076 |
| FJGKIOOH_1 | 1877969 | 1878907 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.30 | 100.00 | NP_391077 |
| FJGKIOOH_1 | 1876322 | 1877941 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.03 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FJGKIOOH_1 | 817928 | 816792 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| FJGKIOOH_1 | 931668 | 930799 | pdxS | 3.01e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| FJGKIOOH_1 | 3336431 | 3335655 | CodY | 3.28e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FJGKIOOH_1 | 1622926 | 1622357 | ClpP | 2.43e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FJGKIOOH_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FJGKIOOH_1 | 3681967 | 3708213 | False | Myoviridae | VOG0753, VOG4705, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)