Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HEBBHNLP_1 | 3769846 | 3771015 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| HEBBHNLP_1 | 3586660 | 3587862 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| HEBBHNLP_1 | 3570096 | 3570950 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| HEBBHNLP_1 | 1799574 | 1800494 | mphK | ARO:3004541 | mphK | 99.35 | 100.00 | CP010314.1 |
| HEBBHNLP_1 | 1786247 | 1787680 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| HEBBHNLP_1 | 1736535 | 1737128 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| HEBBHNLP_1 | 1484301 | 1485947 | vmlR | ARO:3004476 | vmlR | 98.91 | 100.18 | NC_000964.3 |
| HEBBHNLP_1 | 1155465 | 1158035 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| HEBBHNLP_1 | 699018 | 699356 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| HEBBHNLP_1 | 698701 | 699018 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HEBBHNLP_1 | 1799574 | 1800494 | mph(K) | Spiramycin, Telithromycin | 99.46 | 100.00 | NC_000964 |
| HEBBHNLP_1 | 3570096 | 3570950 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HEBBHNLP_1 | 1799577 | 1800494 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.35 | 100.00 | WP_003246254.1 |
| HEBBHNLP_1 | 1484301 | 1485944 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.91 | 100.00 | WP_003234144.1 |
| HEBBHNLP_1 | 3570096 | 3570947 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| HEBBHNLP_1 | 2090120 | 2090638 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
| HEBBHNLP_1 | 4092936 | 4095533 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HEBBHNLP_1 | 2594087 | 2595268 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| HEBBHNLP_1 | 3967560 | 3968201 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| HEBBHNLP_1 | 3016525 | 3017463 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391077 |
| HEBBHNLP_1 | 3013650 | 3014846 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| HEBBHNLP_1 | 3012839 | 3013624 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| HEBBHNLP_1 | 4139491 | 4141254 | dep/capD | 0.0 | Capsule | Immune modulation | 99.09 | 100.00 | NP_389723 |
| HEBBHNLP_1 | 2595751 | 2596893 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| HEBBHNLP_1 | 2595283 | 2595732 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| HEBBHNLP_1 | 3017483 | 3024619 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.60 | 100.00 | NP_391076 |
| HEBBHNLP_1 | 3014875 | 3016497 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.98 | 100.12 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HEBBHNLP_1 | 1931170 | 1930034 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| HEBBHNLP_1 | 2045033 | 2044164 | pdxS | 3.08e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| HEBBHNLP_1 | 388727 | 387951 | CodY | 8.19e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HEBBHNLP_1 | 2747799 | 2747230 | ClpP | 2.49e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HEBBHNLP_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HEBBHNLP_1 | 209989 | 220086 | False | Siphoviridae | VOG0094, VOG9030, VOG0937, VOG7567, VOG4570, VOG5722, VOG4544, VOG5721 |
| HEBBHNLP_1 | 226292 | 246740 | False | Siphoviridae | VOG7718, VOG3390, VOG7895, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG5070, VOG10230 |
| HEBBHNLP_1 | 728594 | 753895 | False | Myoviridae | VOG0136, VOG0753, VOG7895, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)