Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 3 | 16.67 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MPGLFLMH_1 | 3198802 | 3199656 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| MPGLFLMH_1 | 3180419 | 3181621 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| MPGLFLMH_1 | 2983540 | 2984709 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MPGLFLMH_1 | 1997020 | 1997337 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MPGLFLMH_1 | 1996682 | 1997020 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| MPGLFLMH_1 | 1530898 | 1533468 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| MPGLFLMH_1 | 1236677 | 1238320 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| MPGLFLMH_1 | 986281 | 986874 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| MPGLFLMH_1 | 935784 | 937217 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| MPGLFLMH_1 | 922971 | 923891 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MPGLFLMH_1 | 621188 | 622564 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.91 | 100.00 | D12567 |
| MPGLFLMH_1 | 3198804 | 3199656 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| MPGLFLMH_1 | 922971 | 923891 | mph(K) | Spiramycin, Telithromycin | 98.48 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MPGLFLMH_1 | 621191 | 622564 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_063856081.1 |
| MPGLFLMH_1 | 922971 | 923888 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| MPGLFLMH_1 | 1236680 | 1238320 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| MPGLFLMH_1 | 3198805 | 3199656 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| MPGLFLMH_1 | 618717 | 619235 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| MPGLFLMH_1 | 2675750 | 2678347 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.83 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MPGLFLMH_1 | 3797430 | 3798215 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| MPGLFLMH_1 | 3793594 | 3794532 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| MPGLFLMH_1 | 123102 | 124283 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| MPGLFLMH_1 | 3786438 | 3793574 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.58 | 100.00 | NP_391076 |
| MPGLFLMH_1 | 2625214 | 2626977 | dep/capD | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | NP_389723 |
| MPGLFLMH_1 | 3796208 | 3797404 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| MPGLFLMH_1 | 2783578 | 2784219 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| MPGLFLMH_1 | 121477 | 122619 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| MPGLFLMH_1 | 122638 | 123087 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| MPGLFLMH_1 | 3794560 | 3796179 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.33 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MPGLFLMH_1 | 783566 | 784702 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MPGLFLMH_1 | 2310323 | 2311099 | CodY | 3.32e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MPGLFLMH_1 | 4053086 | 4053655 | ClpP | 2.46e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MPGLFLMH_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MPGLFLMH_1 | 1939457 | 1966866 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| MPGLFLMH_1 | 3370122 | 3393924 | False | Myoviridae | VOG4574, VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555, VOG0720, VOG0796 |
Phigaro v2.4.0 (Database: Jan. 2024 release)