Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GMDEOABL_1 | 3683615 | 3686185 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| GMDEOABL_1 | 3209005 | 3209925 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| GMDEOABL_1 | 3195679 | 3197112 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| GMDEOABL_1 | 3146024 | 3146617 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| GMDEOABL_1 | 2893030 | 2894676 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| GMDEOABL_1 | 1744650 | 1745504 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| GMDEOABL_1 | 1726127 | 1727329 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| GMDEOABL_1 | 1529951 | 1531120 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| GMDEOABL_1 | 426674 | 426991 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| GMDEOABL_1 | 426336 | 426674 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GMDEOABL_1 | 1744650 | 1745504 | aadK | Streptomycin | 98.95 | 100.00 | M26879 |
| GMDEOABL_1 | 3209005 | 3209925 | mph(K) | Spiramycin, Telithromycin | 97.83 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GMDEOABL_1 | 2893030 | 2894673 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| GMDEOABL_1 | 1744653 | 1745504 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| GMDEOABL_1 | 3209008 | 3209925 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| GMDEOABL_1 | 3337068 | 3337586 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.22 | 100.00 | WP_003242546.1 |
| GMDEOABL_1 | 1106057 | 1108654 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GMDEOABL_1 | 1332427 | 1333068 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| GMDEOABL_1 | 2341922 | 2343103 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| GMDEOABL_1 | 2750053 | 2750838 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| GMDEOABL_1 | 2343118 | 2343567 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| GMDEOABL_1 | 2750864 | 2752060 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| GMDEOABL_1 | 1060461 | 1062224 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| GMDEOABL_1 | 2753736 | 2754674 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| GMDEOABL_1 | 2343586 | 2344728 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| GMDEOABL_1 | 2754694 | 2761830 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.46 | 100.00 | NP_391076 |
| GMDEOABL_1 | 2752089 | 2753708 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GMDEOABL_1 | 4017116 | 4015980 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| GMDEOABL_1 | 4115642 | 4114773 | pdxS | 1.28e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| GMDEOABL_1 | 738210 | 738986 | CodY | 3.62e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GMDEOABL_1 | 2485078 | 2484509 | ClpP | 2.68e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GMDEOABL_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GMDEOABL_1 | 370482 | 396499 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG5384, VOG4705 |
| GMDEOABL_1 | 1284065 | 1303658 | False | Myoviridae | VOG2278, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712 |
| GMDEOABL_1 | 3937640 | 3961617 | False | Siphoviridae | VOG4574, VOG0136, VOG0753, VOG4728, VOG5535, VOG4599, VOG4605, VOG4545, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG0815, VOG1125, VOG4553, VOG4555, VOG6778, VOG4564, VOG0720, VOG10227, VOG0796 |
| GMDEOABL_1 | 3967297 | 3978876 | False | Siphoviridae | VOG0189, VOG4566, VOG1309, VOG4600, VOG4603, VOG4552, VOG0650, VOG4603, VOG9667, VOG9607, VOG4602, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)