Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 33 | 39.66 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HICLADAD_40 | 19688 | 20557 | rfbA | 0.0 | Capsule | Immune modulation | 99.89 | 100.00 | WP_011681286 |
| HICLADAD_40 | 20557 | 21150 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| HICLADAD_1 | 147103 | 147912 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_002948020 |
| HICLADAD_40 | 20557 | 21150 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| HICLADAD_36 | 91360 | 93018 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| HICLADAD_40 | 20557 | 21150 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226149 |
| HICLADAD_1 | 148849 | 149997 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | WP_041828290 |
| HICLADAD_1 | 147896 | 148852 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226328 |
| HICLADAD_1 | 143044 | 144826 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.10 | 99.89 | WP_011226325 |
| HICLADAD_39 | 13460 | 14200 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | WP_011226046 |
| HICLADAD_1 | 145898 | 147103 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | WP_011226326 |
| HICLADAD_39 | 13460 | 14200 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | WP_011681174 |
| HICLADAD_39 | 81637 | 82429 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.49 | 100.13 | - |
| HICLADAD_1 | 144823 | 145878 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 98.48 | 100.00 | WP_002948022 |
| HICLADAD_1 | 151421 | 152272 | rfbD | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_014621820 |
| HICLADAD_39 | 13465 | 14200 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.23 | 98.13 | WP_011227246 |
| HICLADAD_24 | 87470 | 88429 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.04 | 99.90 | WP_082302295 |
| HICLADAD_39 | 15643 | 17103 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.74 | 100.00 | WP_011681177 |
| HICLADAD_39 | 14911 | 15642 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.68 | 100.00 | WP_011681176 |
| HICLADAD_39 | 14911 | 15642 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.68 | 100.00 | WP_011226048 |
| HICLADAD_4 | 14822 | 15583 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| HICLADAD_39 | 14911 | 15642 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011227248 |
| HICLADAD_39 | 14210 | 14902 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 97.26 | 100.00 | WP_011681175 |
| HICLADAD_39 | 15643 | 17103 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.58 | 100.21 | WP_011226049 |
| HICLADAD_39 | 15643 | 17103 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.76 | 100.21 | WP_041827043 |
| HICLADAD_39 | 12038 | 13403 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.09 | 99.85 | WP_011681173 |
| HICLADAD_39 | 12036 | 13403 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.01 | 100.00 | WP_011226045 |
| HICLADAD_39 | 14210 | 14902 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 90.62 | 100.00 | WP_011226047 |
| HICLADAD_39 | 14210 | 14902 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 90.04 | 100.00 | WP_011227247 |
| HICLADAD_13 | 94525 | 95828 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| HICLADAD_14 | 14535 | 15545 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.46 | 99.80 | WP_002986042 |
| HICLADAD_3 | 54139 | 55422 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| HICLADAD_40 | 21579 | 22625 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HICLADAD_4 | 18270 | 19253 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.51 | 100.00 | AAK75326 |
| HICLADAD_11 | 25102 | 26175 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| HICLADAD_2 | 120998 | 118695 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| HICLADAD_13 | 12215 | 13183 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| HICLADAD_1 | 47882 | 49363 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| HICLADAD_9 | 11752 | 9506 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| HICLADAD_7 | 2344 | 1346 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| HICLADAD_7 | 48025 | 47054 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| HICLADAD_1 | 195291 | 194140 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| HICLADAD_1 | 166898 | 164910 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| HICLADAD_4 | 2073 | 343 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| HICLADAD_37 | 28847 | 30325 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| HICLADAD_13 | 94525 | 95826 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| HICLADAD_37 | 44036 | 45928 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| HICLADAD_9 | 6582 | 8291 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| HICLADAD_12 | 2274 | 1264 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| HICLADAD_36 | 70195 | 71556 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| HICLADAD_38 | 50004 | 48112 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| HICLADAD_13 | 80088 | 80978 | bglB | 1.21e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| HICLADAD_6 | 37056 | 37994 | SP_0676 | 9.74e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| HICLADAD_9 | 9314 | 8457 | metF | 1.38e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| HICLADAD_39 | 53950 | 53246 | covR | 1.97e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| HICLADAD_1 | 10011 | 9340 | rr01 | 3.63e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| HICLADAD_39 | 53950 | 53246 | walR | 8.32e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| HICLADAD_9 | 2628 | 1960 | CiaR | 3.24e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| HICLADAD_35 | 48180 | 47494 | CovR | 7.56e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| HICLADAD_9 | 2628 | 1957 | ciaR | 3.18e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| HICLADAD_7 | 1070 | 696 | SpxA2 | 3.27e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| HICLADAD_36 | 58832 | 58431 | SpxA1 | 2.16e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| HICLADAD_35 | 25798 | 25511 | spr0479 | 1.07e-48 | essential gene | 86.46 | 98.97 | AAK99283 |
| HICLADAD_24 | 129193 | 129447 | spr1327 | 2.40e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| HICLADAD_37 | 77664 | 77401 | spr0175 | 3.42e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| HICLADAD_37 | 76837 | 76571 | spr0177 | 7.47e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| HICLADAD_3 | 53981 | 53481 | RopE (M5005_Spy_1611) | 1.61e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| HICLADAD_2 | 120998 | 118701 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| HICLADAD_38 | 35544 | 34525 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HICLADAD_1 | 0.0 | 1.0 | 0.0 |
| HICLADAD_2 | 0.0 | 1.0 | 0.0 |
| HICLADAD_3 | 0.0 | 1.0 | 0.0 |
| HICLADAD_4 | 0.0 | 1.0 | 0.0 |
| HICLADAD_5 | 0.0 | 1.0 | 0.0 |
| HICLADAD_6 | 0.0 | 1.0 | 0.0 |
| HICLADAD_7 | 0.0 | 1.0 | 0.0 |
| HICLADAD_8 | 0.0 | 1.0 | 0.0 |
| HICLADAD_9 | 0.0 | 1.0 | 0.0 |
| HICLADAD_10 | 0.0 | 1.0 | 0.0 |
| HICLADAD_11 | 0.0 | 1.0 | 0.0 |
| HICLADAD_12 | 0.0 | 1.0 | 0.0 |
| HICLADAD_13 | 0.0 | 1.0 | 0.0 |
| HICLADAD_14 | 0.0 | 1.0 | 0.0 |
| HICLADAD_15 | 0.0 | 1.0 | 0.0 |
| HICLADAD_16 | 0.0 | 1.0 | 0.0 |
| HICLADAD_17 | 0.0 | 1.0 | 0.0 |
| HICLADAD_19 | 0.0 | 1.0 | 0.0 |
| HICLADAD_20 | 0.0 | 1.0 | 0.0 |
| HICLADAD_21 | 0.0 | 1.0 | 0.0 |
| HICLADAD_22 | 0.0 | 1.0 | 0.0 |
| HICLADAD_23 | 0.0 | 1.0 | 0.0 |
| HICLADAD_24 | 0.0 | 1.0 | 0.0 |
| HICLADAD_25 | 0.0 | 1.0 | 0.0 |
| HICLADAD_26 | 0.0 | 1.0 | 0.0 |
| HICLADAD_27 | 0.0 | 1.0 | 0.0 |
| HICLADAD_28 | 1.0 | 0.0 | 1.0 |
| HICLADAD_35 | 0.0 | 1.0 | 0.0 |
| HICLADAD_36 | 0.0 | 1.0 | 0.0 |
| HICLADAD_37 | 0.0 | 1.0 | 0.0 |
| HICLADAD_38 | 0.0 | 1.0 | 0.0 |
| HICLADAD_39 | 0.0 | 1.0 | 0.0 |
| HICLADAD_40 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)