Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KDBKKJHM_8 | 258016 | 260586 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| KDBKKJHM_34 | 112772 | 113692 | mphK | ARO:3004541 | mphK | 94.77 | 100.00 | CP010314.1 |
| KDBKKJHM_9 | 109499 | 109816 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| KDBKKJHM_9 | 109161 | 109499 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KDBKKJHM_34 | 99442 | 100881 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.42 | JYFL01000006.1 |
| KDBKKJHM_34 | 49943 | 50536 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| KDBKKJHM_4 | 30497 | 31351 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| KDBKKJHM_28 | 29029 | 30675 | vmlR | ARO:3004476 | vmlR | 97.63 | 100.18 | NC_000964.3 |
| KDBKKJHM_37 | 16998 | 18167 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| KDBKKJHM_4 | 15466 | 16668 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KDBKKJHM_4 | 30497 | 31351 | aadK | Streptomycin | 98.95 | 100.00 | M26879 |
| KDBKKJHM_34 | 112772 | 113692 | mph(K) | Spiramycin, Telithromycin | 95.55 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KDBKKJHM_4 | 30500 | 31351 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| KDBKKJHM_28 | 29029 | 30672 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 97.63 | 100.00 | WP_003234144.1 |
| KDBKKJHM_34 | 112775 | 113692 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 94.77 | 100.00 | WP_003246254.1 |
| KDBKKJHM_12 | 190490 | 191008 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.33 | 100.00 | WP_003242546.1 |
| KDBKKJHM_3 | 83248 | 85845 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.17 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KDBKKJHM_36 | 3309 | 4490 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| KDBKKJHM_11 | 322906 | 323844 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391077 |
| KDBKKJHM_7 | 15208 | 15848 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 99.84 | NP_390062 |
| KDBKKJHM_11 | 319234 | 320019 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| KDBKKJHM_11 | 323864 | 331000 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.44 | 100.00 | NP_391076 |
| KDBKKJHM_36 | 2845 | 3294 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| KDBKKJHM_3 | 134360 | 136123 | dep/capD | 0.0 | Capsule | Immune modulation | 99.09 | 100.00 | NP_389723 |
| KDBKKJHM_36 | 1684 | 2826 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| KDBKKJHM_11 | 321259 | 322878 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.15 | 100.00 | NP_391078 |
| KDBKKJHM_11 | 320045 | 321241 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.08 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KDBKKJHM_31 | 28032 | 26896 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| KDBKKJHM_25 | 11341 | 10472 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KDBKKJHM_3 | 347537 | 346761 | CodY | 4.04e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KDBKKJHM_11 | 36749 | 36180 | ClpP | 2.30e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| KDBKKJHM_32 | 51858 | 52250 | spxA1 | 4.06e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| KDBKKJHM_32 | 51858 | 52250 | spx | 2.42e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| KDBKKJHM_41 | 20838 | 21080 | SpoVG | 7.54e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| KDBKKJHM_39 | 162419 | 162616 | CspA | 8.86e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| KDBKKJHM_7 | 27811 | 28005 | CspD | 6.99e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| KDBKKJHM_6 | 68540 | 68346 | CspB | 1.03e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KDBKKJHM_20 | 3615 | 3808 | Col(SD853) | 194 / 194 | 97.94 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KDBKKJHM_2 | 1.0 | 0.0 | 1.0 |
| KDBKKJHM_3 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_4 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_5 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_6 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_7 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_8 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_9 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_10 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_11 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_12 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_14 | 1.0 | 0.0 | 1.0 |
| KDBKKJHM_15 | 1.0 | 0.0 | 1.0 |
| KDBKKJHM_16 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_17 | 1.0 | 0.0 | 1.0 |
| KDBKKJHM_18 | 1.0 | 0.0 | 1.0 |
| KDBKKJHM_19 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_20 | 1.0 | 0.0 | 1.0 |
| KDBKKJHM_21 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_22 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_23 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_24 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_25 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_26 | 1.0 | 0.0 | 1.0 |
| KDBKKJHM_27 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_28 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_29 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_30 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_31 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_32 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_33 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_34 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_35 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_36 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_37 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_38 | 1.0 | 0.0 | 1.0 |
| KDBKKJHM_39 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_40 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_41 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_43 | 0.0 | 1.0 | 0.0 |
| KDBKKJHM_44 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KDBKKJHM_9 | 54932 | 79918 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705, VOG0753 |
| KDBKKJHM_11 | 224271 | 252930 | False | Siphoviridae | VOG4566, VOG9600, VOG0025, VOG1884, VOG0198, VOG4841, VOG4581, VOG1886, VOG3644, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG4545, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641, VOG4574 |
Phigaro v2.4.0 (Database: Jan. 2024 release)