Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BNMOBNGM_22 | 690205 | 691374 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BNMOBNGM_30 | 490892 | 491230 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BNMOBNGM_30 | 490575 | 490892 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BNMOBNGM_22 | 463056 | 464258 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| BNMOBNGM_22 | 444735 | 445589 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| BNMOBNGM_37 | 144099 | 144692 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| BNMOBNGM_37 | 93621 | 95054 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| BNMOBNGM_37 | 80807 | 81727 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| BNMOBNGM_34 | 30497 | 32143 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| BNMOBNGM_32 | 27401 | 29971 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BNMOBNGM_42 | 27869 | 29245 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.84 | 100.00 | X08034 |
| BNMOBNGM_22 | 444735 | 445587 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| BNMOBNGM_37 | 80807 | 81727 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BNMOBNGM_42 | 27872 | 29245 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| BNMOBNGM_34 | 30497 | 32140 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| BNMOBNGM_22 | 444735 | 445586 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| BNMOBNGM_37 | 80807 | 81724 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| BNMOBNGM_42 | 25398 | 25916 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| BNMOBNGM_22 | 982890 | 985487 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BNMOBNGM_26 | 109308 | 110246 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| BNMOBNGM_26 | 516844 | 518025 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| BNMOBNGM_26 | 110274 | 111893 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| BNMOBNGM_26 | 113144 | 113929 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| BNMOBNGM_22 | 888677 | 889318 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| BNMOBNGM_26 | 515219 | 516361 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| BNMOBNGM_26 | 111922 | 113118 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| BNMOBNGM_22 | 1030665 | 1032428 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| BNMOBNGM_26 | 102152 | 109288 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.82 | 100.00 | NP_391076 |
| BNMOBNGM_26 | 516380 | 516829 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BNMOBNGM_2 | 28089 | 26953 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BNMOBNGM_11 | 11283 | 10414 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BNMOBNGM_30 | 181025 | 180249 | CodY | 7.44e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BNMOBNGM_26 | 375439 | 376008 | ClpP | 5.80e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BNMOBNGM_30 | 642722 | 642330 | spxA1 | 5.85e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BNMOBNGM_30 | 642722 | 642330 | spx | 3.49e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BNMOBNGM_5 | 20797 | 21039 | SpoVG | 7.54e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BNMOBNGM_34 | 78898 | 78701 | CspA | 9.30e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BNMOBNGM_2 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_5 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_9 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_10 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_11 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_12 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_16 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_20 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_22 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_23 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_25 | 1.0 | 0.0 | 1.0 |
| BNMOBNGM_26 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_28 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_29 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_30 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_31 | 1.0 | 0.0 | 1.0 |
| BNMOBNGM_32 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_34 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_36 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_37 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_41 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_42 | 0.0 | 1.0 | 0.0 |
| BNMOBNGM_44 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BNMOBNGM_22 | 482935 | 513198 | False | Myoviridae | VOG11060, VOG0189, VOG4693, VOG4567, VOG4673, VOG7060, VOG0796, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| BNMOBNGM_30 | 520710 | 548193 | False | Myoviridae | VOG0753, VOG4705, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| BNMOBNGM_41 | 70587 | 97249 | False | Siphoviridae | VOG7134, VOG9867, VOG6036, VOG5495, VOG9219, VOG9713, VOG6940, VOG5534, VOG7718, VOG4705, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG7900, VOG4707 |
Phigaro v2.4.0 (Database: Jan. 2024 release)