Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 1 | 8 | 8.60 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GGJKJGJI_183 | 62 | 832 | TEM-229 | ARO:3005260 | TEM-229 | 100.00 | 89.51 | NG_056416.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GGJKJGJI_298 | 104 | 719 | ant(9)-Ia | Spectinomycin | 100.00 | 80.21 | M69221 |
| GGJKJGJI_183 | 1 | 832 | blaTEM-116 | Amoxicillin, Ampicillin, Cephalothin, Piperacillin, Ticarcillin | 99.64 | 96.63 | AY425988 |
| GGJKJGJI_1 | 278997 | 280373 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 87.15 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GGJKJGJI_183 | 74 | 832 | blaTEM | TEM family class A beta-lactamase | PARTIALX | 100.00 | 88.46 | WP_000027050.1 |
| GGJKJGJI_298 | 107 | 718 | ant(9)-Ib | aminoglycoside nucleotidyltransferase ANT(9)-Ib | PARTIAL_CONTIG_ENDX | 100.00 | 80.00 | WP_063840673.1 |
| GGJKJGJI_1 | 278997 | 280367 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GGJKJGJI_225 | 97849 | 99030 | capB | 0.0 | Capsule | Immune modulation | 82.29 | 99.66 | NP_391471 |
| GGJKJGJI_141 | 1257 | 2524 | AAA22342 | 0.0 | Insecticidal crystalline toxins | Exotoxin | 99.53 | 66.67 | AAA22342 |
| GGJKJGJI_141 | 1257 | 2524 | cry2Ag | 0.0 | Insecticidal crystalline toxins | Exotoxin | 98.34 | 66.56 | ACH91610 |
| GGJKJGJI_141 | 1257 | 2522 | cry2 | 0.0 | Insecticidal crystalline toxins | Exotoxin | 91.32 | 66.51 | AAF09583 |
| GGJKJGJI_141 | 1257 | 2524 | AAA22335 | 0.0 | Insecticidal crystalline toxins | Exotoxin | 88.96 | 66.67 | AAA22335 |
| GGJKJGJI_141 | 1257 | 2524 | cry2Af1 | 0.0 | Insecticidal crystalline toxins | Exotoxin | 84.29 | 66.72 | ABO30519 |
| GGJKJGJI_141 | 1257 | 2524 | CAA40536 | 0.0 | Insecticidal crystalline toxins | Exotoxin | 82.37 | 66.56 | CAA40536 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GGJKJGJI_37 | 31720 | 32856 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| GGJKJGJI_88 | 4352 | 5221 | pdxS | 1.36e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| GGJKJGJI_426 | 54660 | 55436 | CodY | 4.76e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GGJKJGJI_119 | 418866 | 418474 | spxA1 | 1.46e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| GGJKJGJI_550 | 281 | 457 | yedF | 1.45e-36 | hypothetical transcription factor | 94.92 | 76.62 | AAL20878 |
| GGJKJGJI_628 | 85932 | 85735 | CspA | 1.31e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| GGJKJGJI_119 | 634536 | 634730 | CspB | 1.39e-26 | cold shock protein | 84.61 | 98.48 | CAD00094 |
| GGJKJGJI_628 | 85932 | 85738 | CspR | 1.71e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| GGJKJGJI_326 | 290172 | 291044 | lipA (SAUSA300_0829) | 1.38e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| GGJKJGJI_141 | 424 | 554 | ColRNAI | 131 / 130 | 87.02 | DQ298019 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GGJKJGJI_1 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_2 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_27 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_37 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_47 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_58 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_67 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_77 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_88 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_99 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_109 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_119 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_120 | 1.0 | 0.0 | 1.0 |
| GGJKJGJI_141 | 1.0 | 0.0 | 1.0 |
| GGJKJGJI_152 | 1.0 | 0.0 | 1.0 |
| GGJKJGJI_172 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_183 | 1.0 | 0.0 | 1.0 |
| GGJKJGJI_194 | 1.0 | 0.0 | 1.0 |
| GGJKJGJI_204 | 1.0 | 0.0 | 1.0 |
| GGJKJGJI_214 | 1.0 | 0.0 | 1.0 |
| GGJKJGJI_225 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_326 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_426 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_529 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_628 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_727 | 0.0 | 1.0 | 0.0 |
| GGJKJGJI_824 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GGJKJGJI_119 | 262943 | 289061 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG5216 |
| GGJKJGJI_119 | 352559 | 374223 | False | Siphoviridae | VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581 |
| GGJKJGJI_426 | 253542 | 277730 | False | Siphoviridae | VOG4707, VOG4660, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG7930, VOG8610, VOG5008, VOG3390, VOG7718, VOG5534 |
Phigaro v2.4.0 (Database: Jan. 2024 release)