Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IPKEHEPP_1 | 3925394 | 3926314 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| IPKEHEPP_1 | 3912067 | 3913500 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| IPKEHEPP_1 | 3862423 | 3863016 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| IPKEHEPP_1 | 3619549 | 3621192 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| IPKEHEPP_1 | 3326059 | 3328629 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| IPKEHEPP_1 | 2853229 | 2853567 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| IPKEHEPP_1 | 2852912 | 2853229 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| IPKEHEPP_1 | 1767399 | 1768568 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| IPKEHEPP_1 | 1546760 | 1547962 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| IPKEHEPP_1 | 1528371 | 1529225 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IPKEHEPP_1 | 34476 | 35852 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | D12567 |
| IPKEHEPP_1 | 1528371 | 1529223 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| IPKEHEPP_1 | 3925394 | 3926314 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IPKEHEPP_1 | 34476 | 35849 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_063856081.1 |
| IPKEHEPP_1 | 3619549 | 3621189 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| IPKEHEPP_1 | 1528371 | 1529222 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| IPKEHEPP_1 | 3925397 | 3926314 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| IPKEHEPP_1 | 37832 | 38350 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| IPKEHEPP_1 | 2063553 | 2066150 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IPKEHEPP_1 | 931002 | 931940 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| IPKEHEPP_1 | 518600 | 519781 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IPKEHEPP_1 | 929355 | 930974 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| IPKEHEPP_1 | 1969363 | 1970004 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| IPKEHEPP_1 | 927319 | 928104 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.11 | 100.00 | NP_391080 |
| IPKEHEPP_1 | 928130 | 929326 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| IPKEHEPP_1 | 931960 | 939096 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391076 |
| IPKEHEPP_1 | 520264 | 521406 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| IPKEHEPP_1 | 2111328 | 2113091 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| IPKEHEPP_1 | 519796 | 520245 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IPKEHEPP_1 | 4030425 | 4029289 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IPKEHEPP_1 | 4131837 | 4130968 | pdxS | 3.08e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IPKEHEPP_1 | 2543220 | 2542444 | CodY | 8.18e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IPKEHEPP_1 | 661200 | 660631 | ClpP | 2.49e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IPKEHEPP_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IPKEHEPP_1 | 1315676 | 1318334 | False | Unknown | VOG4600, VOG1309 |
| IPKEHEPP_1 | 1324617 | 1351055 | False | Myoviridae | VOG0796, VOG10227, VOG0720, VOG4555, VOG4553, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG1350, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG0753, VOG0136, VOG4574, VOG6422, VOG6116 |
| IPKEHEPP_1 | 1573321 | 1595879 | False | Myoviridae | VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| IPKEHEPP_1 | 2257640 | 2262802 | False | Unknown | VOG4551, VOG0025, VOG7476, VOG0322 |
| IPKEHEPP_1 | 2883048 | 2909531 | False | Myoviridae | VOG0753, VOG4705, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)