Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OPMHJOBB_32 | 648929 | 650098 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| OPMHJOBB_16 | 591897 | 592235 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OPMHJOBB_16 | 591580 | 591897 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| OPMHJOBB_32 | 475632 | 476834 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| OPMHJOBB_32 | 458835 | 459689 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| OPMHJOBB_42 | 263917 | 266487 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| OPMHJOBB_52 | 136473 | 137066 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| OPMHJOBB_58 | 92131 | 93777 | vmlR | ARO:3004476 | vmlR | 98.91 | 100.18 | NC_000964.3 |
| OPMHJOBB_52 | 85975 | 87414 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| OPMHJOBB_52 | 73160 | 74080 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OPMHJOBB_52 | 73160 | 74080 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| OPMHJOBB_32 | 458835 | 459689 | aadK | Streptomycin | 98.48 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OPMHJOBB_58 | 92134 | 93777 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.91 | 100.00 | WP_003234144.1 |
| OPMHJOBB_52 | 73160 | 74077 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| OPMHJOBB_32 | 458835 | 459686 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| OPMHJOBB_23 | 38129 | 38647 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| OPMHJOBB_32 | 942411 | 945008 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OPMHJOBB_32 | 846774 | 847415 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| OPMHJOBB_23 | 523530 | 524711 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| OPMHJOBB_23 | 954761 | 955546 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| OPMHJOBB_23 | 955572 | 956768 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| OPMHJOBB_23 | 958444 | 959382 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| OPMHJOBB_23 | 525194 | 526336 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| OPMHJOBB_32 | 997463 | 999226 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| OPMHJOBB_23 | 524726 | 525175 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| OPMHJOBB_23 | 959402 | 966538 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.54 | 100.00 | NP_391076 |
| OPMHJOBB_23 | 956797 | 958416 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.61 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OPMHJOBB_55 | 28025 | 26889 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| OPMHJOBB_25 | 4374 | 5243 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| OPMHJOBB_16 | 280544 | 279768 | CodY | 5.14e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OPMHJOBB_23 | 682751 | 682182 | ClpP | 6.42e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| OPMHJOBB_47 | 275728 | 276120 | spxA1 | 2.72e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| OPMHJOBB_47 | 275728 | 276120 | spx | 1.62e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| OPMHJOBB_66 | 20589 | 20831 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| OPMHJOBB_58 | 44897 | 45094 | CspA | 5.30e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| OPMHJOBB_47 | 31059 | 30865 | CspB | 3.92e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OPMHJOBB_16 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_19 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_20 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_22 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_23 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_25 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_26 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_32 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_42 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_47 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_52 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_55 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_58 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_66 | 0.0 | 1.0 | 0.0 |
| OPMHJOBB_73 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OPMHJOBB_16 | 622953 | 629113 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894 |
| OPMHJOBB_32 | 302474 | 336202 | False | Siphoviridae | VOG7438, VOG1047, VOG0021, VOG1565, VOG0026, VOG4609, VOG0327, VOG4548, VOG0198, VOG4841, VOG4581, VOG4544, VOG3644, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG5852, VOG5910, VOG4545, VOG0801, VOG4619, VOG4626, VOG4612, VOG3390, VOG4705, VOG0641, VOG4574 |
| OPMHJOBB_47 | 312782 | 316663 | False | Unknown | VOG3304, VOG9667, VOG9667, VOG8241, VOG4552 |
| OPMHJOBB_47 | 327526 | 348047 | False | Siphoviridae | VOG0796, VOG10227, VOG0720, VOG4564, VOG4555, VOG4553, VOG1125, VOG0815, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG4545, VOG4605, VOG4599, VOG5535 |
| OPMHJOBB_47 | 412603 | 420806 | False | Unknown | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773 |
Phigaro v2.4.0 (Database: Jan. 2024 release)