Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 7 | 17.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EOBFACIF_3 | 1205505 | 1208075 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| EOBFACIF_3 | 1137468 | 1137806 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| EOBFACIF_3 | 1137151 | 1137468 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| EOBFACIF_4 | 827646 | 828848 | blt | ARO:3003006 | blt | 98.50 | 100.00 | L32599.1 |
| EOBFACIF_4 | 809429 | 810271 | aadK | ARO:3002627 | aadK | 97.83 | 98.59 | AL009126.1 |
| EOBFACIF_1 | 188778 | 190424 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| EOBFACIF_2 | 170936 | 171529 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| EOBFACIF_3 | 164219 | 165388 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| EOBFACIF_2 | 120416 | 121849 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| EOBFACIF_2 | 107603 | 108523 | mphK | ARO:3004541 | mphK | 99.34 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EOBFACIF_2 | 107603 | 108523 | mph(K) | Spiramycin, Telithromycin | 99.24 | 100.00 | NC_000964 |
| EOBFACIF_4 | 809408 | 810260 | aadK | Streptomycin | 98.12 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EOBFACIF_2 | 107603 | 108511 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.34 | 99.02 | WP_003246254.1 |
| EOBFACIF_1 | 188781 | 190424 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| EOBFACIF_4 | 809408 | 810259 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| EOBFACIF_4 | 320644 | 321162 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| EOBFACIF_3 | 454005 | 456602 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EOBFACIF_3 | 509127 | 510890 | dep/capD | 0.0 | Capsule | Immune modulation | 99.94 | 100.00 | NP_389723 |
| EOBFACIF_1 | 809747 | 810928 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| EOBFACIF_3 | 362778 | 363419 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| EOBFACIF_1 | 387914 | 388699 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| EOBFACIF_1 | 386692 | 387888 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| EOBFACIF_1 | 384078 | 385016 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| EOBFACIF_1 | 808122 | 809264 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| EOBFACIF_1 | 809283 | 809732 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| EOBFACIF_1 | 376922 | 384058 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391076 |
| EOBFACIF_1 | 385044 | 386663 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.97 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EOBFACIF_5 | 28062 | 26926 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| EOBFACIF_2 | 14485 | 13616 | pdxS | 1.71e-150 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EOBFACIF_3 | 826017 | 825241 | CodY | 1.20e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EOBFACIF_1 | 651319 | 651888 | ClpP | 5.92e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| EOBFACIF_6 | 276070 | 276462 | spxA1 | 2.45e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| EOBFACIF_6 | 276070 | 276462 | spx | 1.46e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| EOBFACIF_1 | 239979 | 240221 | SpoVG | 1.34e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| EOBFACIF_6 | 32079 | 31885 | CspB | 3.54e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EOBFACIF_1 | 0.0 | 1.0 | 0.0 |
| EOBFACIF_2 | 0.0 | 1.0 | 0.0 |
| EOBFACIF_3 | 0.0 | 1.0 | 0.0 |
| EOBFACIF_4 | 0.0 | 1.0 | 0.0 |
| EOBFACIF_5 | 0.0 | 1.0 | 0.0 |
| EOBFACIF_6 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EOBFACIF_3 | 1166227 | 1174020 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885 |
| EOBFACIF_4 | 849769 | 878659 | False | Myoviridae | VOG1309, VOG0189, VOG4693, VOG4673, VOG0796, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| EOBFACIF_6 | 369586 | 379509 | False | Siphoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685 |
Phigaro v2.4.0 (Database: Jan. 2024 release)