Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 0 | 0 | 12.00 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CNEMKCMN_1 | 3498983 | 3499948 | CfxA4 | ARO:3003005 | CfxA4 | 100.00 | 100.00 | AY769933.1 |
| CNEMKCMN_1 | 1691894 | 1693099 | Mef(En2) | ARO:3004659 | Mef(En2) | 99.50 | 100.00 | AF251288.1 |
| CNEMKCMN_1 | 1574074 | 1575999 | tet(Q) | ARO:3000191 | tet(Q) | 99.06 | 97.56 | Z21523.1 |
| CNEMKCMN_1 | 1568161 | 1568964 | APH(3'')-Ib | ARO:3002639 | APH(3'')-Ib | 99.63 | 100.00 | AF313472.2 |
| CNEMKCMN_1 | 1567323 | 1568174 | sul2 | ARO:3000412 | sul2 | 100.00 | 104.43 | AY055428.1 |
| CNEMKCMN_3 | 3884 | 4684 | ErmF | ARO:3000498 | ErmF | 99.62 | 100.00 | M17124.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| CNEMKCMN_1 | 1533188 | 1534054 | ant(6)-Ia | Streptomycin | 100.00 | 100.00 | KF864551 |
| CNEMKCMN_1 | 3498983 | 3499948 | cfxA4 | Unknown Beta-lactam | 100.00 | 100.00 | AY769933 |
| CNEMKCMN_3 | 3884 | 4684 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | M17808 |
| CNEMKCMN_1 | 1567323 | 1568174 | sul2 | Sulfamethoxazole | 100.00 | 100.00 | FJ197818 |
| CNEMKCMN_1 | 1570141 | 1570950 | aph(3')-Ib | Kanamycin, Neomycin, Kanamycin, Lividomycin, Paromomycin, Ribostamycin | 99.88 | 99.26 | AJ744860 |
| CNEMKCMN_1 | 1568964 | 1569590 | aph(6)-Id | Streptomycin | 99.84 | 74.91 | 18676889 |
| CNEMKCMN_3 | 2742 | 3671 | tet(X) | Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.68 | 79.52 | M37699 |
| CNEMKCMN_1 | 1574074 | 1575999 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.38 | 100.00 | Z21523 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CNEMKCMN_1 | 1534985 | 1535758 | aad9 | ANT(9) family aminoglycoside nucleotidyltransferase | EXACTX | 100.00 | 100.00 | WP_002578722.1 |
| CNEMKCMN_1 | 1533191 | 1534054 | aadE | aminoglycoside 6-adenylyltransferase AadE | EXACTX | 100.00 | 100.00 | WP_001255868.1 |
| CNEMKCMN_1 | 1568161 | 1568961 | aph(3'')-Ib | aminoglycoside O-phosphotransferase APH(3'')-Ib | EXACTX | 100.00 | 100.00 | WP_001082319.1 |
| CNEMKCMN_1 | 3498986 | 3499948 | cfxA4 | extended-spectrum class A beta-lactamase CfxA4 | EXACTX | 100.00 | 100.00 | WP_057280848.1 |
| CNEMKCMN_3 | 3887 | 4684 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | EXACTX | 100.00 | 100.00 | WP_002682030.1 |
| CNEMKCMN_1 | 1693124 | 1693633 | lnu(AN2) | lincosamide nucleotidyltransferase Lnu(AN2) | EXACTX | 100.00 | 100.00 | WP_004308783.1 |
| CNEMKCMN_1 | 1567323 | 1568171 | sul2 | sulfonamide-resistant dihydropteroate synthase Sul2 | EXACTX | 100.00 | 100.00 | WP_011270145.1 |
| CNEMKCMN_1 | 1568964 | 1569590 | aph(6)-Id | aminoglycoside O-phosphotransferase APH(6)-Id | PARTIALX | 100.00 | 75.18 | WP_000480968.1 |
| CNEMKCMN_3 | 2751 | 3668 | tet(X2) | tetracycline-inactivating monooxygenase Tet(X2) | PARTIALX | 99.67 | 78.87 | WP_008651082.1 |
| CNEMKCMN_1 | 1570144 | 1570950 | aph(3')-Ib | aminoglycoside O-phosphotransferase APH(3')-Ib | BLASTX | 99.63 | 99.26 | WP_011205810.1 |
| CNEMKCMN_1 | 1691894 | 1693096 | mef(En2) | macrolide efflux MFS transporter Mef(En2) | BLASTX | 99.50 | 100.00 | WP_063853729.1 |
| CNEMKCMN_1 | 1574074 | 1575996 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.06 | 100.00 | WP_002560998.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| CNEMKCMN_5 | 7 | 587 | repUS2 | 584 / 681 | 80.48 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| CNEMKCMN_1 | 0.0 | 1.0 | 0.0 |
| CNEMKCMN_2 | 1.0 | 0.0 | 1.0 |
| CNEMKCMN_3 | 1.0 | 0.0 | 1.0 |
| CNEMKCMN_4 | 1.0 | 0.0 | 1.0 |
| CNEMKCMN_5 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| CNEMKCMN_1 | 1430849 | 1441955 | False | Siphoviridae | VOG1563, |
| CNEMKCMN_1 | 2389496 | 2395799 | False | Unknown | VOG1134, |
Phigaro v2.4.0 (Database: Jan. 2024 release)