Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 4 | 15.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BMAEBEMH_1 | 2587998 | 2588852 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| BMAEBEMH_1 | 2569230 | 2570432 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| BMAEBEMH_1 | 2396456 | 2397625 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| BMAEBEMH_1 | 1372912 | 1373229 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BMAEBEMH_1 | 1372574 | 1372912 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| BMAEBEMH_1 | 895074 | 897644 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| BMAEBEMH_1 | 299364 | 300797 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BMAEBEMH_1 | 4074375 | 4075751 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.77 | 100.00 | D12567 |
| BMAEBEMH_1 | 2588000 | 2588852 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| BMAEBEMH_1 | 273087 | 274006 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BMAEBEMH_1 | 4074378 | 4075751 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| BMAEBEMH_1 | 594316 | 595956 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.62 | 100.00 | WP_003234144.1 |
| BMAEBEMH_1 | 2588001 | 2588852 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| BMAEBEMH_1 | 4071882 | 4072400 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BMAEBEMH_1 | 3148014 | 3148952 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| BMAEBEMH_1 | 3569646 | 3570827 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BMAEBEMH_1 | 3568021 | 3569163 | capA | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391469 |
| BMAEBEMH_1 | 3569182 | 3569631 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| BMAEBEMH_1 | 3151839 | 3152624 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| BMAEBEMH_1 | 1913729 | 1915492 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| BMAEBEMH_1 | 3140857 | 3147994 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.77 | 100.00 | NP_391076 |
| BMAEBEMH_1 | 3150617 | 3151813 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| BMAEBEMH_1 | 2193264 | 2193905 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| BMAEBEMH_1 | 3148980 | 3150599 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BMAEBEMH_1 | 132546 | 133682 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| BMAEBEMH_1 | 18677 | 19546 | pdxS | 5.34e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| BMAEBEMH_1 | 1699242 | 1700018 | CodY | 3.33e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BMAEBEMH_1 | 3414664 | 3415233 | ClpP | 2.47e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BMAEBEMH_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BMAEBEMH_1 | 1317485 | 1343351 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG3892, VOG6163, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705, VOG0753 |
| BMAEBEMH_1 | 2142060 | 2162155 | True | Unknown | VOG4705, VOG5008, VOG8610, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG4660, VOG4579, VOG0944 |
Phigaro v2.4.0 (Database: Jan. 2024 release)