Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OBPCBFAH_11 | 609208 | 610062 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| OBPCBFAH_11 | 590016 | 591218 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| OBPCBFAH_13 | 514585 | 514923 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OBPCBFAH_13 | 514268 | 514585 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| OBPCBFAH_11 | 363370 | 364539 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| OBPCBFAH_16 | 197263 | 198906 | vmlR | ARO:3004476 | vmlR | 98.90 | 100.00 | NC_000964.3 |
| OBPCBFAH_17 | 108135 | 109055 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
| OBPCBFAH_17 | 94807 | 96240 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| OBPCBFAH_17 | 45086 | 45679 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| OBPCBFAH_15 | 27423 | 29993 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.30 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OBPCBFAH_17 | 108135 | 109055 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| OBPCBFAH_12 | 1008744 | 1010120 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.77 | 100.00 | D12567 |
| OBPCBFAH_11 | 609210 | 610062 | aadK | Streptomycin | 98.12 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OBPCBFAH_16 | 197266 | 198906 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.90 | 100.00 | WP_003234144.1 |
| OBPCBFAH_17 | 108138 | 109055 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| OBPCBFAH_12 | 1008747 | 1010120 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| OBPCBFAH_11 | 609211 | 610062 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| OBPCBFAH_12 | 1006223 | 1006741 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| OBPCBFAH_11 | 59677 | 62274 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OBPCBFAH_12 | 527328 | 528509 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| OBPCBFAH_12 | 101976 | 109112 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391076 |
| OBPCBFAH_11 | 164235 | 164876 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| OBPCBFAH_12 | 525703 | 526845 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| OBPCBFAH_12 | 112968 | 113753 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| OBPCBFAH_12 | 526864 | 527313 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| OBPCBFAH_12 | 109132 | 110070 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| OBPCBFAH_12 | 111746 | 112942 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| OBPCBFAH_11 | 9596 | 11359 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| OBPCBFAH_12 | 110098 | 111717 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.07 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OBPCBFAH_19 | 31880 | 33016 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| OBPCBFAH_2 | 11283 | 10414 | pdxS | 1.13e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| OBPCBFAH_13 | 204773 | 203997 | CodY | 4.51e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OBPCBFAH_12 | 365972 | 366541 | ClpP | 6.31e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| OBPCBFAH_14 | 276344 | 276736 | spxA1 | 9.29e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| OBPCBFAH_1 | 20722 | 20964 | SpoVG | 7.53e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| OBPCBFAH_16 | 142798 | 142995 | CspA | 9.85e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| OBPCBFAH_14 | 33148 | 32954 | CspB | 3.55e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OBPCBFAH_1 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_2 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_3 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_4 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_5 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_8 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_9 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_11 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_12 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_13 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_14 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_15 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_16 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_17 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_18 | 0.0 | 1.0 | 0.0 |
| OBPCBFAH_19 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OBPCBFAH_11 | 541548 | 571397 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796, VOG4673, VOG4693, VOG0189, VOG11060, VOG1309 |
| OBPCBFAH_11 | 750789 | 776935 | False | Siphoviridae | VOG4615, VOG0136, VOG5221, VOG4705, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG4841, VOG0713, VOG11015, VOG0198 |
| OBPCBFAH_11 | 832011 | 856128 | False | Myoviridae | VOG6116, VOG4574, VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555, VOG0720 |
| OBPCBFAH_11 | 863628 | 865158 | False | Unknown | VOG0189 |
| OBPCBFAH_13 | 544407 | 552880 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885 |
| OBPCBFAH_14 | 371174 | 381499 | False | Siphoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749 |
Phigaro v2.4.0 (Database: Jan. 2024 release)