Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CBBMCBHD_118 | 155183 | 155776 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| CBBMCBHD_129 | 114988 | 115326 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| CBBMCBHD_129 | 114671 | 114988 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| CBBMCBHD_118 | 104626 | 106065 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| CBBMCBHD_118 | 91813 | 92733 | mphK | ARO:3004541 | mphK | 99.35 | 100.00 | CP010314.1 |
| CBBMCBHD_58 | 85455 | 86309 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| CBBMCBHD_85 | 68715 | 69884 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| CBBMCBHD_61 | 49842 | 51488 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| CBBMCBHD_55 | 27472 | 30042 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| CBBMCBHD_83 | 389 | 1591 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| CBBMCBHD_118 | 91813 | 92733 | mph(K) | Spiramycin, Telithromycin | 99.46 | 100.00 | NC_000964 |
| CBBMCBHD_58 | 85455 | 86309 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CBBMCBHD_118 | 91813 | 92730 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.35 | 100.00 | WP_003246254.1 |
| CBBMCBHD_61 | 49845 | 51488 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| CBBMCBHD_58 | 85455 | 86306 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| CBBMCBHD_60 | 38221 | 38739 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.22 | 100.00 | WP_003242546.1 |
| CBBMCBHD_73 | 3393 | 5990 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| CBBMCBHD_74 | 23710 | 24891 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| CBBMCBHD_63 | 36090 | 36875 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| CBBMCBHD_85 | 266548 | 267189 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| CBBMCBHD_63 | 34868 | 36064 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| CBBMCBHD_68 | 8382 | 10145 | dep/capD | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | NP_389723 |
| CBBMCBHD_63 | 32254 | 33192 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| CBBMCBHD_74 | 22085 | 23227 | capA | 0.0 | Capsule | Immune modulation | 98.86 | 100.00 | NP_391469 |
| CBBMCBHD_74 | 23246 | 23695 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| CBBMCBHD_63 | 25098 | 32234 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.42 | 100.00 | NP_391076 |
| CBBMCBHD_63 | 33220 | 34839 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.35 | 100.00 | NP_391078 |
| CBBMCBHD_6 | 1 | 467 | pilF | 0.0 | Type IV pili | Adherence | 99.14 | 61.53 | NP_252494 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| CBBMCBHD_65 | 28132 | 26996 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| CBBMCBHD_76 | 11332 | 10463 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| CBBMCBHD_107 | 196439 | 197215 | CodY | 1.93e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| CBBMCBHD_63 | 299478 | 300047 | ClpP | 2.61e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| CBBMCBHD_140 | 111372 | 111764 | spxA1 | 1.12e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| CBBMCBHD_140 | 111372 | 111764 | spx | 6.66e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| CBBMCBHD_66 | 34796 | 34554 | SpoVG | 7.53e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| CBBMCBHD_61 | 2649 | 2846 | CspA | 3.48e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| CBBMCBHD_85 | 255316 | 255122 | CspD | 2.16e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| CBBMCBHD_151 | 32120 | 31926 | CspB | 1.52e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| CBBMCBHD_11 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_22 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_33 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_44 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_55 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_58 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_59 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_60 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_61 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_62 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_63 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_64 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_65 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_66 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_67 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_68 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_69 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_70 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_71 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_72 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_73 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_74 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_75 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_76 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_77 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_78 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_79 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_80 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_81 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_82 | 1.0 | 0.0 | 1.0 |
| CBBMCBHD_83 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_84 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_85 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_87 | 1.0 | 0.0 | 1.0 |
| CBBMCBHD_88 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_89 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_90 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_93 | 1.0 | 0.0 | 1.0 |
| CBBMCBHD_94 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_96 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_107 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_118 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_129 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_140 | 0.0 | 1.0 | 0.0 |
| CBBMCBHD_151 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| CBBMCBHD_44 | 17594 | 21997 | False | Unknown | VOG6211, VOG0322, VOG7476 |
| CBBMCBHD_85 | 322294 | 323791 | False | Unknown | VOG7930, VOG8610, VOG7895 |
| CBBMCBHD_85 | 325441 | 331164 | False | Siphoviridae | VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036, VOG9867 |
| CBBMCBHD_129 | 144877 | 171189 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)