Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JMMOACNJ_4 | 205221 | 206390 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| JMMOACNJ_25 | 92373 | 94943 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| JMMOACNJ_11 | 86753 | 87346 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| JMMOACNJ_50 | 48725 | 50371 | vmlR | ARO:3004476 | vmlR | 98.18 | 100.18 | NC_000964.3 |
| JMMOACNJ_11 | 36194 | 37627 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| JMMOACNJ_4 | 26535 | 27737 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| JMMOACNJ_11 | 23380 | 24300 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| JMMOACNJ_4 | 9987 | 10841 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| JMMOACNJ_37 | 4262 | 4579 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| JMMOACNJ_37 | 3924 | 4262 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| JMMOACNJ_11 | 23380 | 24300 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| JMMOACNJ_4 | 9987 | 10841 | aadK | Streptomycin | 98.60 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JMMOACNJ_11 | 23380 | 24288 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| JMMOACNJ_4 | 9987 | 10838 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| JMMOACNJ_50 | 48728 | 50371 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.18 | 100.00 | WP_003234144.1 |
| JMMOACNJ_70 | 38094 | 38612 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
| JMMOACNJ_17 | 29771 | 32368 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.41 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JMMOACNJ_16 | 1302 | 1943 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| JMMOACNJ_58 | 11953 | 13134 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| JMMOACNJ_33 | 35258 | 36043 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| JMMOACNJ_33 | 34036 | 35232 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| JMMOACNJ_33 | 31422 | 32360 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| JMMOACNJ_58 | 13617 | 14759 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| JMMOACNJ_17 | 80108 | 81871 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| JMMOACNJ_58 | 13149 | 13598 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| JMMOACNJ_33 | 24266 | 31402 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.49 | 100.00 | NP_391076 |
| JMMOACNJ_33 | 32388 | 34007 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.67 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JMMOACNJ_28 | 27940 | 26804 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| JMMOACNJ_56 | 4283 | 5152 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| JMMOACNJ_7 | 132494 | 133270 | CodY | 2.62e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| JMMOACNJ_21 | 77237 | 77806 | ClpP | 1.25e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| JMMOACNJ_57 | 36378 | 35986 | spxA1 | 2.84e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| JMMOACNJ_57 | 36378 | 35986 | spx | 1.69e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| JMMOACNJ_10 | 34592 | 34350 | SpoVG | 6.11e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| JMMOACNJ_82 | 443 | 246 | CspA | 6.21e-36 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| JMMOACNJ_16 | 13173 | 13367 | CspD | 4.88e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| JMMOACNJ_34 | 31987 | 31793 | CspB | 6.23e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JMMOACNJ_1 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_2 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_3 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_4 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_5 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_6 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_7 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_8 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_9 | 1.0 | 0.0 | 1.0 |
| JMMOACNJ_10 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_11 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_12 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_13 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_14 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_15 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_16 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_17 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_18 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_20 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_21 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_22 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_23 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_24 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_25 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_26 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_27 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_28 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_29 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_30 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_31 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_32 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_33 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_34 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_35 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_36 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_37 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_38 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_39 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_40 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_41 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_42 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_43 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_44 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_45 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_46 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_47 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_49 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_50 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_51 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_52 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_53 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_54 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_55 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_56 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_57 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_58 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_59 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_60 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_61 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_62 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_64 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_65 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_66 | 1.0 | 0.0 | 1.0 |
| JMMOACNJ_67 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_68 | 1.0 | 0.0 | 1.0 |
| JMMOACNJ_69 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_70 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_71 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_72 | 1.0 | 0.0 | 1.0 |
| JMMOACNJ_73 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_74 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_75 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_76 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_77 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_78 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_79 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_83 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_85 | 1.0 | 0.0 | 1.0 |
| JMMOACNJ_87 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_88 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_89 | 0.0 | 1.0 | 0.0 |
| JMMOACNJ_90 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JMMOACNJ_12 | 156426 | 185681 | False | Siphoviridae | VOG7531, VOG0705, VOG6116, VOG8440, VOG0602, VOG4574, VOG0753, VOG4705, VOG4626, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5601, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841, VOG0531, VOG9437, VOG0274 |
| JMMOACNJ_12 | 203883 | 218847 | False | Siphoviridae | VOG0026, VOG6497, VOG10937, VOG5753, VOG0052, VOG1563, VOG11003, VOG2215, VOG4551, VOG0025, VOG0044, VOG0189, VOG0705, VOG6168 |
| JMMOACNJ_16 | 153252 | 177672 | False | Siphoviridae | VOG4873, VOG2352, VOG1030, VOG0676, VOG1093, VOG5543, VOG4877, VOG8598, VOG0052, VOG4551, VOG0025, VOG0189, VOG6168, VOG10230, VOG0641, VOG0565, VOG4599, VOG4605, VOG4545, VOG5716 |
| JMMOACNJ_22 | 5906 | 15070 | False | Siphoviridae | VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490 |
| JMMOACNJ_46 | 4165 | 10711 | False | Myoviridae | VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
Phigaro v2.4.0 (Database: Jan. 2024 release)