safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
12	10	8	17.55

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
MDCGABEN_206	58109	59548	lmrB	ARO:3002813	lmrB	99.37	100.42	JYFL01000006.1
MDCGABEN_206	45296	46216	mphK	ARO:3004541	mphK	99.02	100.00	CP010314.1
MDCGABEN_173	42004	44574	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	99.53	100.00	AL009126.3
MDCGABEN_135	38229	38822	tmrB	ARO:3003059	tmrB	98.48	100.00	AL009126.3
MDCGABEN_225	32179	32496	ykkD	ARO:3003064	ykkD	99.05	100.00	AL009126.1
MDCGABEN_225	31841	32179	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
MDCGABEN_174	14933	16576	vmlR	ARO:3004476	vmlR	98.35	100.00	NC_000964.3
MDCGABEN_149	14878	15732	aadK	ARO:3002627	aadK	98.59	100.00	AL009126.1
MDCGABEN_151	12488	13657	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
MDCGABEN_74	355	1557	blt	ARO:3003006	blt	99.00	100.00	L32599.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
MDCGABEN_206	45296	46216	mph(K)	Spiramycin, Telithromycin	99.35	100.00	NC_000964
MDCGABEN_149	14878	15732	aadK	Streptomycin	98.71	100.00	M26879

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
MDCGABEN_206	45296	46213	mphK	macrolide 2'-phosphotransferase MphK	BLASTX	99.02	100.00	WP_003246254.1
MDCGABEN_149	14878	15729	aadK	aminoglycoside 6-adenylyltransferase AadK	BLASTX	98.59	100.00	WP_003229862.1
MDCGABEN_174	14933	16573	vmlR	ABC-F type ribosomal protection protein VmlR	BLASTX	98.35	100.00	WP_003234144.1
MDCGABEN_182	1071	1589	satA	streptothricin N-acetyltransferase SatA	BLASTX	94.22	100.00	WP_003242546.1
MDCGABEN_16	410	3007	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	80.65	100.00	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
MDCGABEN_210	682	1863	capB	Capsule	Immune modulation	99.75	100.00	NP_391471
MDCGABEN_44	14913	15698	dhbA	Bacillibactin	Nutritional/Metabolic factor	99.75	100.00	NP_391080
MDCGABEN_221	5951	6592	hlyIII	Hemolysin III	Exotoxin	99.69	100.00	NP_390062
MDCGABEN_44	13691	14887	dhbC	Bacillibactin	Nutritional/Metabolic factor	99.33	100.00	NP_391079
MDCGABEN_40	39053	40816	dep/capD	Capsule	Immune modulation	99.32	100.00	NP_389723
MDCGABEN_210	1878	2327	capC	Capsule	Immune modulation	99.11	100.00	NP_391470
MDCGABEN_210	2346	3488	capA	Capsule	Immune modulation	98.78	100.00	NP_391469
MDCGABEN_44	3921	11057	dhbF	Bacillibactin	Nutritional/Metabolic factor	98.74	100.00	NP_391076
MDCGABEN_44	11077	12015	dhbB	Bacillibactin	Nutritional/Metabolic factor	98.40	100.00	NP_391077
MDCGABEN_44	12043	13662	dhbE	Bacillibactin	Nutritional/Metabolic factor	97.41	100.00	NP_391078

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
MDCGABEN_227	28148	27012	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
MDCGABEN_218	11244	10375	pdxS	8.09e-152	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
MDCGABEN_5	942	166	CodY	5.75e-127	nutrient-responsive regulator	81.08	100.00	ACJ80679
MDCGABEN_165	13106	13675	ClpP	1.20e-100	part of proteolytic complex	81.05	97.44	BAB94595
MDCGABEN_198	8011	8403	spxA1	2.76e-71	redox-responsive transcription factor	83.97	100.00	AAF21893
MDCGABEN_198	8011	8403	spx	1.65e-69	essential gene which is a thiol/oxidative stress sensor	80.15	100.00	BAB57159
MDCGABEN_11	5932	6174	SpoVG	5.51e-39	RNA-binding protein	81.48	79.41	AAT02994
MDCGABEN_181	7480	7677	CspA	3.49e-33	cold shock protein	90.91	100.00	CAA62903
MDCGABEN_52	1630	1824	CspD	2.56e-29	cold shock protein	80.00	98.48	CAC99957
MDCGABEN_52	1630	1827	CspB	5.32e-29	cold shock protein	80.30	100.00	CAD00094

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
MDCGABEN_1	0.0	1.0	0.0
MDCGABEN_2	0.0	1.0	0.0
MDCGABEN_3	0.0	1.0	0.0
MDCGABEN_4	0.0	1.0	0.0
MDCGABEN_5	0.0	1.0	0.0
MDCGABEN_6	0.0	1.0	0.0
MDCGABEN_7	0.0	1.0	0.0
MDCGABEN_8	0.0	1.0	0.0
MDCGABEN_9	0.0	1.0	0.0
MDCGABEN_10	0.0	1.0	0.0
MDCGABEN_11	0.0	1.0	0.0
MDCGABEN_12	0.0	1.0	0.0
MDCGABEN_13	0.0	1.0	0.0
MDCGABEN_14	0.0	1.0	0.0
MDCGABEN_15	0.0	1.0	0.0
MDCGABEN_16	1.0	0.0	1.0
MDCGABEN_17	0.0	1.0	0.0
MDCGABEN_18	0.0	1.0	0.0
MDCGABEN_19	0.0	1.0	0.0
MDCGABEN_20	0.0	1.0	0.0
MDCGABEN_21	0.0	1.0	0.0
MDCGABEN_22	1.0	0.0	1.0
MDCGABEN_23	0.0	1.0	0.0
MDCGABEN_24	0.0	1.0	0.0
MDCGABEN_25	0.0	1.0	0.0
MDCGABEN_26	0.0	1.0	0.0
MDCGABEN_27	0.0	1.0	0.0
MDCGABEN_28	0.0	1.0	0.0
MDCGABEN_29	0.0	1.0	0.0
MDCGABEN_30	0.0	1.0	0.0
MDCGABEN_31	0.0	1.0	0.0
MDCGABEN_32	0.0	1.0	0.0
MDCGABEN_33	0.0	1.0	0.0
MDCGABEN_34	0.0	1.0	0.0
MDCGABEN_35	0.0	1.0	0.0
MDCGABEN_36	0.0	1.0	0.0
MDCGABEN_37	0.0	1.0	0.0
MDCGABEN_38	0.0	1.0	0.0
MDCGABEN_39	0.0	1.0	0.0
MDCGABEN_40	0.0	1.0	0.0
MDCGABEN_41	0.0	1.0	0.0
MDCGABEN_42	0.0	1.0	0.0
MDCGABEN_43	0.0	1.0	0.0
MDCGABEN_44	0.0	1.0	0.0
MDCGABEN_45	0.0	1.0	0.0
MDCGABEN_46	0.0	1.0	0.0
MDCGABEN_47	0.0	1.0	0.0
MDCGABEN_48	0.0	1.0	0.0
MDCGABEN_49	0.0	1.0	0.0
MDCGABEN_50	0.0	1.0	0.0
MDCGABEN_51	0.0	1.0	0.0
MDCGABEN_52	0.0	1.0	0.0
MDCGABEN_54	0.0	1.0	0.0
MDCGABEN_55	1.0	0.0	1.0
MDCGABEN_56	0.0	1.0	0.0
MDCGABEN_57	0.0	1.0	0.0
MDCGABEN_58	0.0	1.0	0.0
MDCGABEN_59	0.0	1.0	0.0
MDCGABEN_60	0.0	1.0	0.0
MDCGABEN_61	0.0	1.0	0.0
MDCGABEN_62	0.0	1.0	0.0
MDCGABEN_63	0.0	1.0	0.0
MDCGABEN_64	0.0	1.0	0.0
MDCGABEN_65	0.0	1.0	0.0
MDCGABEN_66	0.0	1.0	0.0
MDCGABEN_67	0.0	1.0	0.0
MDCGABEN_68	0.0	1.0	0.0
MDCGABEN_69	0.0	1.0	0.0
MDCGABEN_70	0.0	1.0	0.0
MDCGABEN_71	1.0	0.0	1.0
MDCGABEN_73	0.0	1.0	0.0
MDCGABEN_74	0.0	1.0	0.0
MDCGABEN_75	0.0	1.0	0.0
MDCGABEN_76	1.0	0.0	1.0
MDCGABEN_77	0.0	1.0	0.0
MDCGABEN_78	1.0	0.0	1.0
MDCGABEN_79	0.0	1.0	0.0
MDCGABEN_80	0.0	1.0	0.0
MDCGABEN_81	0.0	1.0	0.0
MDCGABEN_82	1.0	0.0	1.0
MDCGABEN_83	0.0	1.0	0.0
MDCGABEN_84	1.0	0.0	1.0
MDCGABEN_85	0.0	1.0	0.0
MDCGABEN_86	0.0	1.0	0.0
MDCGABEN_87	0.0	1.0	0.0
MDCGABEN_88	0.0	1.0	0.0
MDCGABEN_89	0.0	1.0	0.0
MDCGABEN_90	0.0	1.0	0.0
MDCGABEN_91	0.0	1.0	0.0
MDCGABEN_92	0.0	1.0	0.0
MDCGABEN_93	0.0	1.0	0.0
MDCGABEN_94	0.0	1.0	0.0
MDCGABEN_95	0.0	1.0	0.0
MDCGABEN_96	0.0	1.0	0.0
MDCGABEN_97	0.0	1.0	0.0
MDCGABEN_98	1.0	0.0	1.0
MDCGABEN_99	0.0	1.0	0.0
MDCGABEN_101	0.0	1.0	0.0
MDCGABEN_102	1.0	0.0	1.0
MDCGABEN_103	0.0	1.0	0.0
MDCGABEN_104	0.0	1.0	0.0
MDCGABEN_106	0.0	1.0	0.0
MDCGABEN_108	0.0	1.0	0.0
MDCGABEN_109	0.0	1.0	0.0
MDCGABEN_111	1.0	0.0	1.0
MDCGABEN_112	0.0	1.0	0.0
MDCGABEN_113	0.0	1.0	0.0
MDCGABEN_114	0.0	1.0	0.0
MDCGABEN_115	0.0	1.0	0.0
MDCGABEN_116	0.0	1.0	0.0
MDCGABEN_119	0.0	1.0	0.0
MDCGABEN_120	0.0	1.0	0.0
MDCGABEN_121	0.0	1.0	0.0
MDCGABEN_122	1.0	0.0	1.0
MDCGABEN_123	0.0	1.0	0.0
MDCGABEN_124	0.0	1.0	0.0
MDCGABEN_127	0.0	1.0	0.0
MDCGABEN_128	0.0	1.0	0.0
MDCGABEN_129	1.0	0.0	1.0
MDCGABEN_131	0.0	1.0	0.0
MDCGABEN_134	0.0	1.0	0.0
MDCGABEN_135	0.0	1.0	0.0
MDCGABEN_141	0.0	1.0	0.0
MDCGABEN_144	0.0	1.0	0.0
MDCGABEN_145	0.0	1.0	0.0
MDCGABEN_146	0.0	1.0	0.0
MDCGABEN_147	0.0	1.0	0.0
MDCGABEN_148	0.0	1.0	0.0
MDCGABEN_149	0.0	1.0	0.0
MDCGABEN_150	0.0	1.0	0.0
MDCGABEN_151	0.0	1.0	0.0
MDCGABEN_152	0.0	1.0	0.0
MDCGABEN_153	0.0	1.0	0.0
MDCGABEN_155	0.0	1.0	0.0
MDCGABEN_156	0.0	1.0	0.0
MDCGABEN_158	0.0	1.0	0.0
MDCGABEN_159	0.0	1.0	0.0
MDCGABEN_160	1.0	0.0	1.0
MDCGABEN_161	0.0	1.0	0.0
MDCGABEN_162	0.0	1.0	0.0
MDCGABEN_163	0.0	1.0	0.0
MDCGABEN_164	0.0	1.0	0.0
MDCGABEN_165	0.0	1.0	0.0
MDCGABEN_166	0.0	1.0	0.0
MDCGABEN_167	0.0	1.0	0.0
MDCGABEN_168	0.0	1.0	0.0
MDCGABEN_169	0.0	1.0	0.0
MDCGABEN_171	0.0	1.0	0.0
MDCGABEN_172	0.0	1.0	0.0
MDCGABEN_173	0.0	1.0	0.0
MDCGABEN_174	0.0	1.0	0.0
MDCGABEN_175	0.0	1.0	0.0
MDCGABEN_176	0.0	1.0	0.0
MDCGABEN_177	0.0	1.0	0.0
MDCGABEN_178	0.0	1.0	0.0
MDCGABEN_179	0.0	1.0	0.0
MDCGABEN_180	0.0	1.0	0.0
MDCGABEN_181	0.0	1.0	0.0
MDCGABEN_182	0.0	1.0	0.0
MDCGABEN_183	0.0	1.0	0.0
MDCGABEN_184	0.0	1.0	0.0
MDCGABEN_185	0.0	1.0	0.0
MDCGABEN_186	0.0	1.0	0.0
MDCGABEN_187	0.0	1.0	0.0
MDCGABEN_188	1.0	0.0	1.0
MDCGABEN_189	0.0	1.0	0.0
MDCGABEN_190	0.0	1.0	0.0
MDCGABEN_191	0.0	1.0	0.0
MDCGABEN_192	0.0	1.0	0.0
MDCGABEN_193	0.0	1.0	0.0
MDCGABEN_194	0.0	1.0	0.0
MDCGABEN_195	0.0	1.0	0.0
MDCGABEN_196	0.0	1.0	0.0
MDCGABEN_197	0.0	1.0	0.0
MDCGABEN_198	0.0	1.0	0.0
MDCGABEN_199	0.0	1.0	0.0
MDCGABEN_200	0.0	1.0	0.0
MDCGABEN_201	0.0	1.0	0.0
MDCGABEN_202	0.0	1.0	0.0
MDCGABEN_203	0.0	1.0	0.0
MDCGABEN_204	0.0	1.0	0.0
MDCGABEN_205	0.0	1.0	0.0
MDCGABEN_206	0.0	1.0	0.0
MDCGABEN_207	0.0	1.0	0.0
MDCGABEN_208	0.0	1.0	0.0
MDCGABEN_209	0.0	1.0	0.0
MDCGABEN_210	0.0	1.0	0.0
MDCGABEN_211	0.0	1.0	0.0
MDCGABEN_212	0.0	1.0	0.0
MDCGABEN_213	0.0	1.0	0.0
MDCGABEN_214	0.0	1.0	0.0
MDCGABEN_215	0.0	1.0	0.0
MDCGABEN_216	0.0	1.0	0.0
MDCGABEN_217	0.0	1.0	0.0
MDCGABEN_218	0.0	1.0	0.0
MDCGABEN_219	0.0	1.0	0.0
MDCGABEN_220	0.0	1.0	0.0
MDCGABEN_221	0.0	1.0	0.0
MDCGABEN_222	0.0	1.0	0.0
MDCGABEN_223	0.0	1.0	0.0
MDCGABEN_224	0.0	1.0	0.0
MDCGABEN_225	0.0	1.0	0.0
MDCGABEN_226	0.0	1.0	0.0
MDCGABEN_227	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
MDCGABEN_9	1722	19468	False	Myoviridae	VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227