Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DCGKMBIL_35 | 277926 | 278243 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DCGKMBIL_35 | 277588 | 277926 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DCGKMBIL_36 | 261230 | 262150 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| DCGKMBIL_36 | 247903 | 249336 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| DCGKMBIL_4 | 240902 | 242104 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| DCGKMBIL_36 | 198184 | 198777 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| DCGKMBIL_4 | 62221 | 63390 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DCGKMBIL_5 | 29032 | 30678 | vmlR | ARO:3004476 | vmlR | 98.18 | 100.18 | NC_000964.3 |
| DCGKMBIL_7 | 27360 | 29930 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| DCGKMBIL_39 | 8843 | 9697 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DCGKMBIL_36 | 261230 | 262150 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| DCGKMBIL_39 | 8843 | 9697 | aadK | Streptomycin | 98.60 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DCGKMBIL_36 | 261242 | 262150 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| DCGKMBIL_39 | 8846 | 9697 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| DCGKMBIL_5 | 29032 | 30675 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.18 | 100.00 | WP_003234144.1 |
| DCGKMBIL_12 | 38095 | 38613 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
| DCGKMBIL_13 | 51729 | 54326 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.41 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DCGKMBIL_16 | 54657 | 55298 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| DCGKMBIL_23 | 390147 | 391328 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| DCGKMBIL_2 | 36144 | 36929 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| DCGKMBIL_2 | 34922 | 36118 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| DCGKMBIL_2 | 32308 | 33246 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| DCGKMBIL_23 | 391811 | 392953 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| DCGKMBIL_13 | 2226 | 3989 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| DCGKMBIL_23 | 391343 | 391792 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| DCGKMBIL_2 | 25152 | 32288 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.49 | 100.00 | NP_391076 |
| DCGKMBIL_2 | 33274 | 34893 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.67 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DCGKMBIL_34 | 31676 | 32812 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DCGKMBIL_25 | 4284 | 5153 | pdxS | 1.10e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DCGKMBIL_1 | 202311 | 201535 | CodY | 2.71e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DCGKMBIL_2 | 288498 | 289067 | ClpP | 2.55e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DCGKMBIL_35 | 129798 | 130190 | spxA1 | 2.91e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DCGKMBIL_35 | 129798 | 130190 | spx | 1.74e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DCGKMBIL_11 | 34593 | 34351 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DCGKMBIL_5 | 79924 | 79727 | CspA | 3.89e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DCGKMBIL_3 | 153100 | 152906 | CspD | 4.07e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| DCGKMBIL_3 | 153100 | 152903 | CspB | 8.43e-28 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DCGKMBIL_1 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_2 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_3 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_4 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_5 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_7 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_8 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_9 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_10 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_11 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_12 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_13 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_15 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_16 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_17 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_18 | 1.0 | 0.0 | 1.0 |
| DCGKMBIL_19 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_20 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_21 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_22 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_23 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_25 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_26 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_28 | 1.0 | 0.0 | 1.0 |
| DCGKMBIL_29 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_30 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_32 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_33 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_34 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_35 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_36 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_37 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_38 | 0.0 | 1.0 | 0.0 |
| DCGKMBIL_39 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DCGKMBIL_3 | 8027 | 13021 | False | Siphoviridae | VOG5543, VOG1093, VOG0676, VOG1030, VOG2352 |
| DCGKMBIL_35 | 221043 | 248073 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| DCGKMBIL_39 | 176654 | 205290 | False | Siphoviridae | VOG0705, VOG6116, VOG8440, VOG0602, VOG4574, VOG0753, VOG4705, VOG4626, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5601, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841, VOG0531, VOG9437, VOG0274 |
| DCGKMBIL_39 | 223492 | 238456 | False | Siphoviridae | VOG0026, VOG6497, VOG10937, VOG5753, VOG0052, VOG1563, VOG11003, VOG2215, VOG4551, VOG0025, VOG0044, VOG0189, VOG0705, VOG6168 |
Phigaro v2.4.0 (Database: Jan. 2024 release)