Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 8 | 17.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JKFCFLAI_16 | 721575 | 722429 | aadK | ARO:3002627 | aadK | 97.18 | 100.00 | AL009126.1 |
| JKFCFLAI_16 | 702237 | 703439 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| JKFCFLAI_16 | 479568 | 480737 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| JKFCFLAI_11 | 462282 | 462620 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| JKFCFLAI_11 | 461965 | 462282 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| JKFCFLAI_5 | 109578 | 110498 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| JKFCFLAI_5 | 96279 | 97712 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| JKFCFLAI_7 | 91064 | 92692 | vmlR | ARO:3004476 | vmlR | 95.20 | 99.09 | NC_000964.3 |
| JKFCFLAI_5 | 46561 | 47154 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| JKFCFLAI_3 | 27385 | 29955 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| JKFCFLAI_16 | 721575 | 722429 | aadK | Streptomycin | 98.13 | 100.00 | M26879 |
| JKFCFLAI_5 | 109578 | 110498 | mph(K) | Spiramycin, Telithromycin | 97.07 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JKFCFLAI_5 | 109581 | 110498 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| JKFCFLAI_16 | 721578 | 722429 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.18 | 100.00 | WP_003229862.1 |
| JKFCFLAI_7 | 91070 | 92692 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 95.38 | 98.90 | WP_003234144.1 |
| JKFCFLAI_14 | 41219 | 41731 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.23 | 98.84 | WP_003242546.1 |
| JKFCFLAI_16 | 54529 | 57126 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JKFCFLAI_13 | 316761 | 317942 | capB | 0.0 | Capsule | Immune modulation | 99.41 | 100.00 | NP_391471 |
| JKFCFLAI_13 | 317957 | 318406 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| JKFCFLAI_13 | 318425 | 319567 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| JKFCFLAI_6 | 112902 | 113687 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.98 | 100.00 | NP_391080 |
| JKFCFLAI_16 | 278709 | 279350 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.75 | 100.00 | NP_390062 |
| JKFCFLAI_6 | 109066 | 110004 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391077 |
| JKFCFLAI_6 | 111680 | 112876 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.41 | 100.00 | NP_391079 |
| JKFCFLAI_6 | 101916 | 109046 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.32 | 99.99 | NP_391076 |
| JKFCFLAI_16 | 10025 | 11788 | dep/capD | 0.0 | Capsule | Immune modulation | 98.07 | 100.00 | NP_389723 |
| JKFCFLAI_6 | 110032 | 111651 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.59 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JKFCFLAI_9 | 28022 | 26886 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| JKFCFLAI_4 | 4304 | 5173 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| JKFCFLAI_12 | 74024 | 74800 | CodY | 2.90e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| JKFCFLAI_13 | 475582 | 475013 | ClpP | 3.38e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| JKFCFLAI_11 | 619979 | 619587 | spxA1 | 4.81e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| JKFCFLAI_11 | 619979 | 619587 | spx | 2.87e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| JKFCFLAI_19 | 34611 | 34369 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| JKFCFLAI_7 | 37648 | 37845 | CspA | 5.26e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| JKFCFLAI_13 | 59961 | 60155 | CspB | 5.17e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JKFCFLAI_1 | 1.0 | 0.0 | 1.0 |
| JKFCFLAI_2 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_3 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_4 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_5 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_6 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_7 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_8 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_9 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_10 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_11 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_12 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_13 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_14 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_15 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_16 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_18 | 0.0 | 1.0 | 0.0 |
| JKFCFLAI_19 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JKFCFLAI_11 | 493646 | 519760 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| JKFCFLAI_16 | 168414 | 175302 | False | Siphoviridae | VOG0918, VOG4551, VOG0025, VOG7476, VOG0322 |
| JKFCFLAI_16 | 648629 | 681687 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG10989, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG5668, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4585, VOG0198, VOG4058, VOG4693, VOG0189, VOG11060 |
Phigaro v2.4.0 (Database: Jan. 2024 release)