Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 2 | 7 | 7.87 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IMBHKMNO_33 | 391312 | 392361 | clbA | ARO:3002814 | clbA | 97.13 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IMBHKMNO_33 | 391312 | 392347 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.22 | 98.67 | KR610408 |
| IMBHKMNO_11 | 389138 | 390514 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 87.07 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IMBHKMNO_33 | 391312 | 392355 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 97.41 | 99.71 | WP_012116915.1 |
| IMBHKMNO_11 | 389138 | 390508 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
| IMBHKMNO_11 | 1147834 | 1150428 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.23 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IMBHKMNO_22 | 533633 | 534814 | capB | 0.0 | Capsule | Immune modulation | 82.38 | 99.66 | NP_391471 |
| IMBHKMNO_22 | 533169 | 533618 | capC | 3.84e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IMBHKMNO_66 | 31717 | 32853 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| IMBHKMNO_2 | 4309 | 5178 | pdxS | 1.38e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| IMBHKMNO_44 | 211924 | 211148 | CodY | 4.37e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IMBHKMNO_63 | 56353 | 55961 | spxA1 | 6.72e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| IMBHKMNO_33 | 360529 | 360726 | CspA | 2.59e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| IMBHKMNO_63 | 272526 | 272720 | CspB | 6.40e-27 | cold shock protein | 84.61 | 98.48 | CAD00094 |
| IMBHKMNO_33 | 360529 | 360723 | CspR | 3.39e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| IMBHKMNO_22 | 143392 | 142520 | lipA (SAUSA300_0829) | 3.33e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| IMBHKMNO_5 | 2047 | 2214 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IMBHKMNO_1 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_2 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_3 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_4 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_5 | 1.0 | 0.0 | 1.0 |
| IMBHKMNO_6 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_8 | 1.0 | 0.0 | 1.0 |
| IMBHKMNO_10 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_11 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_22 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_33 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_44 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_55 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_63 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_64 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_65 | 0.0 | 1.0 | 0.0 |
| IMBHKMNO_66 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IMBHKMNO_11 | 808198 | 835687 | False | Siphoviridae | VOG2703, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641, VOG10230 |
| IMBHKMNO_44 | 358596 | 364463 | False | Unknown | VOG0585, VOG3549, VOG10904, VOG7718 |
| IMBHKMNO_55 | 265266 | 269517 | False | Myoviridae | VOG4885, VOG5447, VOG4861, VOG0189 |
| IMBHKMNO_63 | 1149 | 6958 | False | Siphoviridae | VOG0204, VOG4553, VOG4568, VOG4556, VOG5051, VOG4544 |
Phigaro v2.4.0 (Database: Jan. 2024 release)