Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EKNAMFOO_1 | 2600584 | 2601438 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| EKNAMFOO_1 | 2583666 | 2584868 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| EKNAMFOO_1 | 2358791 | 2359960 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| EKNAMFOO_1 | 1337608 | 1337925 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| EKNAMFOO_1 | 1337270 | 1337608 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| EKNAMFOO_1 | 873977 | 876547 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| EKNAMFOO_1 | 583111 | 584757 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| EKNAMFOO_1 | 341991 | 342584 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| EKNAMFOO_1 | 291514 | 292947 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| EKNAMFOO_1 | 278701 | 279621 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EKNAMFOO_1 | 4042260 | 4043636 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.84 | 100.00 | X08034 |
| EKNAMFOO_1 | 2600584 | 2601438 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
| EKNAMFOO_1 | 278701 | 279621 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EKNAMFOO_1 | 4042263 | 4043636 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| EKNAMFOO_1 | 2600587 | 2601438 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| EKNAMFOO_1 | 583114 | 584757 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| EKNAMFOO_1 | 278701 | 279618 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| EKNAMFOO_1 | 4039789 | 4040307 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| EKNAMFOO_1 | 2061193 | 2063790 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EKNAMFOO_1 | 3150389 | 3151327 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| EKNAMFOO_1 | 3565759 | 3566940 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| EKNAMFOO_1 | 3151355 | 3152974 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| EKNAMFOO_1 | 3154225 | 3155010 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| EKNAMFOO_1 | 2157340 | 2157981 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| EKNAMFOO_1 | 3564134 | 3565276 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| EKNAMFOO_1 | 3153003 | 3154199 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| EKNAMFOO_1 | 2014252 | 2016015 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| EKNAMFOO_1 | 3143233 | 3150369 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.71 | 100.00 | NP_391076 |
| EKNAMFOO_1 | 3565295 | 3565744 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EKNAMFOO_1 | 132400 | 133536 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| EKNAMFOO_1 | 18665 | 19534 | pdxS | 3.02e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EKNAMFOO_1 | 1647468 | 1648244 | CodY | 3.30e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EKNAMFOO_1 | 3416523 | 3417092 | ClpP | 2.45e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EKNAMFOO_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EKNAMFOO_1 | 1280932 | 1307738 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| EKNAMFOO_1 | 1848540 | 1875336 | False | Siphoviridae | VOG7466, VOG4887, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5221, VOG0136 |
| EKNAMFOO_1 | 2536967 | 2566809 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796, VOG4673, VOG4693, VOG0189, VOG11060, VOG1309 |
Phigaro v2.4.0 (Database: Jan. 2024 release)