safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	7	17.83

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
BHMNOAOC_11	756013	756867	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
BHMNOAOC_11	737304	738506	blt	ARO:3003006	blt	100.00	100.00	L32599.1
BHMNOAOC_11	514985	516154	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
BHMNOAOC_9	423971	424288	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
BHMNOAOC_9	423633	423971	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
BHMNOAOC_7	276350	278920	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
BHMNOAOC_5	165302	165895	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
BHMNOAOC_5	114800	116239	lmrB	ARO:3002813	lmrB	99.79	100.42	JYFL01000006.1
BHMNOAOC_5	101985	102905	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
BHMNOAOC_6	75627	77270	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
BHMNOAOC_11	756013	756867	aadK	Streptomycin	100.00	100.00	M26879
BHMNOAOC_5	101985	102905	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
BHMNOAOC_15	90417	91793	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
BHMNOAOC_11	756016	756867	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
BHMNOAOC_5	101985	102902	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
BHMNOAOC_15	87899	88417	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
BHMNOAOC_15	90420	91793	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
BHMNOAOC_6	75630	77270	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
BHMNOAOC_11	71671	74265	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
BHMNOAOC_13	519199	520341	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
BHMNOAOC_13	520824	522005	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
BHMNOAOC_13	520360	520809	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
BHMNOAOC_11	25013	26776	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
BHMNOAOC_13	112863	113648	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
BHMNOAOC_13	109027	109965	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
BHMNOAOC_13	111641	112837	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
BHMNOAOC_13	109993	111612	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
BHMNOAOC_13	101871	109007	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
BHMNOAOC_11	315639	316280	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BHMNOAOC_3	31711	32847	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
BHMNOAOC_1	4286	5155	pdxS	1.10e-151	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
BHMNOAOC_9	737239	738015	CodY	8.23e-125	nutrient-responsive regulator	81.08	100.00	ACJ80679
BHMNOAOC_13	367587	368156	ClpP	4.08e-99	part of proteolytic complex	81.05	97.44	BAB94595
BHMNOAOC_9	274810	275202	spxA1	2.44e-69	redox-responsive transcription factor	83.21	100.00	AAF21893
BHMNOAOC_2	20553	20795	SpoVG	7.49e-39	RNA-binding protein	81.48	79.41	AAT02994
BHMNOAOC_6	30155	30352	CspA	4.58e-32	cold shock protein	90.91	100.00	CAA62903

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BHMNOAOC_1	0.0	1.0	0.0
BHMNOAOC_2	0.0	1.0	0.0
BHMNOAOC_3	0.0	1.0	0.0
BHMNOAOC_5	0.0	1.0	0.0
BHMNOAOC_6	0.0	1.0	0.0
BHMNOAOC_7	0.0	1.0	0.0
BHMNOAOC_9	0.0	1.0	0.0
BHMNOAOC_10	0.0	1.0	0.0
BHMNOAOC_11	0.0	1.0	0.0
BHMNOAOC_13	0.0	1.0	0.0
BHMNOAOC_14	0.0	1.0	0.0
BHMNOAOC_15	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
BHMNOAOC_9	367686	393794	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
BHMNOAOC_11	687375	717619	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)