Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 7 | 17.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BCBBMCHE_15 | 1596617 | 1597471 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| BCBBMCHE_15 | 1579538 | 1580740 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| BCBBMCHE_15 | 1352770 | 1353939 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BCBBMCHE_15 | 445599 | 445916 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BCBBMCHE_15 | 445261 | 445599 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BCBBMCHE_14 | 269610 | 272180 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| BCBBMCHE_13 | 203491 | 205137 | vmlR | ARO:3004476 | vmlR | 98.91 | 100.18 | NC_000964.3 |
| BCBBMCHE_11 | 155752 | 156345 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| BCBBMCHE_11 | 105194 | 106633 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| BCBBMCHE_11 | 92380 | 93300 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BCBBMCHE_15 | 1596617 | 1597471 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| BCBBMCHE_11 | 92380 | 93300 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BCBBMCHE_13 | 203494 | 205137 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.91 | 100.00 | WP_003234144.1 |
| BCBBMCHE_11 | 92380 | 93288 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| BCBBMCHE_15 | 1596620 | 1597471 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| BCBBMCHE_23 | 1003582 | 1004100 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| BCBBMCHE_15 | 1038229 | 1040817 | rphC | rifamycin-inactivating phosphotransferase RphC | INTERNAL_STOP | 80.25 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BCBBMCHE_15 | 1152033 | 1152674 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| BCBBMCHE_23 | 112776 | 113561 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| BCBBMCHE_23 | 519798 | 520247 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| BCBBMCHE_23 | 520262 | 521443 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| BCBBMCHE_15 | 985089 | 986852 | dep/capD | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | NP_389723 |
| BCBBMCHE_23 | 518637 | 519779 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| BCBBMCHE_23 | 108940 | 109878 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| BCBBMCHE_23 | 111554 | 112750 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| BCBBMCHE_23 | 101784 | 108920 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.99 | 100.00 | NP_391076 |
| BCBBMCHE_23 | 109906 | 111525 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.10 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BCBBMCHE_7 | 31638 | 32774 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BCBBMCHE_1 | 4324 | 5193 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BCBBMCHE_15 | 763727 | 764503 | CodY | 1.34e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BCBBMCHE_23 | 365299 | 365868 | ClpP | 6.25e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BCBBMCHE_15 | 277642 | 278034 | spxA1 | 1.06e-69 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BCBBMCHE_15 | 277642 | 278034 | spx | 6.30e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BCBBMCHE_3 | 20480 | 20722 | SpoVG | 7.47e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BCBBMCHE_13 | 155344 | 155541 | CspA | 1.01e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BCBBMCHE_1 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_3 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_7 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_8 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_9 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_11 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_13 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_14 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_15 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_17 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_18 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_20 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_21 | 0.0 | 1.0 | 0.0 |
| BCBBMCHE_23 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BCBBMCHE_15 | 389092 | 415771 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| BCBBMCHE_15 | 1524553 | 1554451 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600 |
| BCBBMCHE_17 | 517 | 23667 | False | Siphoviridae | VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG4841, VOG2874, VOG0275, VOG0198, VOG4376, VOG6581, VOG0397, VOG4694 |
| BCBBMCHE_17 | 25887 | 28920 | False | Unknown | VOG0189, VOG4566, VOG0650, VOG9667 |
| BCBBMCHE_21 | 1 | 29344 | False | Siphoviridae | VOG4626, VOG4619, VOG0801, VOG4545, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3644, VOG1886, VOG4581, VOG4556, VOG4841, VOG0198, VOG4548, VOG0327, VOG4609, VOG0026, VOG1565, VOG0021, VOG1047, VOG7438 |
Phigaro v2.4.0 (Database: Jan. 2024 release)