Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 6 | 0 | 1 | 6.08 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CDCMBNIM_1 | 1233312 | 1234094 | ANT(9)-Ia | ARO:3002630 | ANT(9)-Ia | 100.00 | 100.00 | X02588.1 |
| CDCMBNIM_1 | 1232455 | 1233186 | ErmA | ARO:3000347 | ErmA | 99.59 | 100.00 | X03216.1 |
| CDCMBNIM_2 | 267269 | 268642 | AAC(6')-Ie-APH(2'')-Ia bifunctional protein | ARO:3002597 | AAC6_Ie_APH2_Ia | 100.00 | 95.41 | GU565967.1 |
| CDCMBNIM_3 | 27682 | 29607 | tet(M) | ARO:3000186 | tet(M) | 98.13 | 100.31 | AB039845.1 |
| CDCMBNIM_3 | 26096 | 27472 | tet(L) | ARO:3000179 | tet(L) | 99.13 | 100.00 | M11036.1 |
| CDCMBNIM_2 | 25152 | 26015 | ANT(6)-Ia | ARO:3002626 | ANT(6)-Ia | 100.00 | 100.00 | KF648874.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| CDCMBNIM_2 | 25152 | 26015 | ant(6)-Ia | Streptomycin | 100.00 | 100.00 | KF421157 |
| CDCMBNIM_2 | 267306 | 268642 | aac(6')-aph(2'') | Gentamicin, Tobramycin, Netilmicin, Kanamycin, Amikacin, Dibekacin, Isepamicin, Fortimicin, Streptomycin | 100.00 | 92.85 | M13771 |
| CDCMBNIM_2 | 267306 | 268642 | aph(2'')-Ia | Amikacin, Gentamicin, Tobramycin, Kanamycin | 100.00 | 90.58 | AP009486 |
| CDCMBNIM_1 | 1233312 | 1234094 | ant(9)-Ia | Spectinomycin | 99.87 | 100.00 | X02588 |
| CDCMBNIM_1 | 1232455 | 1233186 | erm(A) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.86 | 100.00 | X03216 |
| CDCMBNIM_3 | 26096 | 27472 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.78 | 100.00 | M29725 |
| CDCMBNIM_3 | 27682 | 29607 | tet(M) | Doxycycline, Tetracycline, Minocycline | 99.07 | 100.00 | X75073 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CDCMBNIM_2 | 25155 | 26015 | ant(6)-Ia | aminoglycoside nucleotidyltransferase ANT(6)-Ia | EXACTX | 100.00 | 100.00 | WP_002294505.1 |
| CDCMBNIM_1 | 1233315 | 1234094 | ant(9)-Ia | aminoglycoside nucleotidyltransferase ANT(9)-Ia | EXACTX | 100.00 | 100.00 | WP_000067268.1 |
| CDCMBNIM_2 | 267305 | 268642 | aac(6')-Ie/aph(2'')-Ia | bifunctional aminoglycoside N-acetyltransferase AAC(6')-Ie/aminoglycoside O-phosphotransferase APH(2'')-Ia | BLASTX | 100.00 | 93.11 | WP_001028144.1 |
| CDCMBNIM_1 | 1232455 | 1233183 | erm(A) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) | BLASTX | 99.59 | 100.00 | WP_001072201.1 |
| CDCMBNIM_3 | 26099 | 27472 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_001574277.1 |
| CDCMBNIM_3 | 27685 | 29607 | tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | BLASTX | 98.91 | 100.00 | WP_000691736.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| CDCMBNIM_1 | 52985 | 53692 | WalR | 6.85e-115 | transcriptional regulatory protein | 85.59 | 100.43 | EPH95667 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| CDCMBNIM_1 | 0.0 | 1.0 | 0.0 |
| CDCMBNIM_2 | 1.0 | 0.0 | 1.0 |
| CDCMBNIM_3 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| CDCMBNIM_1 | 492495 | 503134 | False | Siphoviridae | VOG4602, VOG8520, VOG8520, VOG7236, VOG5744, VOG7667, VOG0535, VOG0322, VOG6545, VOG0514, VOG4799, VOG0025, VOG6830, VOG9667, VOG5814, VOG5280, VOG5317, VOG0586, VOG5258 |
| CDCMBNIM_1 | 507330 | 530385 | False | Siphoviridae | VOG0198, VOG8681, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG0539, VOG0207, VOG4586, VOG9312, VOG0866, VOG5793, VOG0801, VOG3462, VOG7521, VOG4856, VOG10868, VOG3390, VOG4705 |
| CDCMBNIM_1 | 630641 | 661461 | False | Siphoviridae | VOG0321, VOG4548, VOG0322, VOG6545, VOG4632, VOG4609, VOG0327, VOG0637, VOG0198, VOG0249, VOG3699, VOG4544, VOG0720, VOG4564, VOG3749, VOG4555, VOG1329, VOG4713, VOG0704, VOG0799, VOG0725, VOG0817, VOG6163, VOG0801, VOG3462, VOG6116, VOG4856 |
| CDCMBNIM_1 | 1292330 | 1318655 | False | Siphoviridae | VOG8294, VOG0703, VOG1637, VOG4705, VOG4334, VOG6540, VOG5601, VOG4856, VOG3462, VOG0801, VOG6163, VOG0817, VOG0725, VOG0799, VOG0704, VOG4713, VOG1329, VOG4555, VOG4564, VOG0720, VOG4544, VOG1517, VOG8462, VOG7442, VOG4618, VOG0376, VOG2545 |
| CDCMBNIM_1 | 1324591 | 1330390 | False | Unknown | VOG0186, VOG1309, VOG0198, VOG9949, VOG4552, VOG5353, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)