Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 9 | 17.38 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DOFGBLLO_44 | 157771 | 158364 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| DOFGBLLO_44 | 109890 | 111329 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| DOFGBLLO_28 | 94290 | 94607 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| DOFGBLLO_28 | 93952 | 94290 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| DOFGBLLO_75 | 30659 | 31513 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| DOFGBLLO_75 | 11895 | 13097 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| DOFGBLLO_90 | 9343 | 11913 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| DOFGBLLO_42 | 9052 | 10221 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DOFGBLLO_75 | 30661 | 31513 | aadK | Streptomycin | 98.36 | 99.77 | M26879 |
| DOFGBLLO_44 | 97079 | 97998 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DOFGBLLO_47 | 49127 | 50767 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.81 | 100.00 | WP_003234144.1 |
| DOFGBLLO_75 | 30662 | 31513 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| DOFGBLLO_78 | 87890 | 88408 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DOFGBLLO_39 | 15922 | 17064 | capA | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | NP_391469 |
| DOFGBLLO_39 | 14258 | 15439 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| DOFGBLLO_33 | 60821 | 61759 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391077 |
| DOFGBLLO_39 | 15454 | 15903 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| DOFGBLLO_33 | 57149 | 57934 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| DOFGBLLO_32 | 17619 | 19382 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| DOFGBLLO_33 | 61779 | 68915 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.03 | 100.00 | NP_391076 |
| DOFGBLLO_33 | 57960 | 59156 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| DOFGBLLO_11 | 88560 | 89201 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| DOFGBLLO_33 | 59174 | 60793 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DOFGBLLO_51 | 28006 | 26870 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| DOFGBLLO_79 | 11142 | 10273 | pdxS | 1.93e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| DOFGBLLO_48 | 61458 | 62234 | CodY | 1.40e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DOFGBLLO_56 | 198019 | 198588 | ClpP | 1.57e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DOFGBLLO_54 | 9689 | 10081 | spxA1 | 3.54e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DOFGBLLO_54 | 9689 | 10081 | spx | 2.11e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DOFGBLLO_30 | 20492 | 20734 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DOFGBLLO_47 | 3979 | 4176 | CspA | 1.37e-31 | cold shock protein | 89.39 | 100.00 | CAA62903 |
| DOFGBLLO_11 | 76708 | 76514 | CspD | 3.12e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| DOFGBLLO_61 | 31226 | 31032 | CspB | 4.07e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
| DOFGBLLO_47 | 3979 | 4173 | CspR | 4.30e-27 | cold shock protein | 80.00 | 98.48 | AAO82613 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DOFGBLLO_1 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_2 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_11 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_14 | 1.0 | 0.0 | 1.0 |
| DOFGBLLO_18 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_24 | 1.0 | 0.0 | 1.0 |
| DOFGBLLO_26 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_28 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_29 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_30 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_31 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_32 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_33 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_34 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_35 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_36 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_37 | 1.0 | 0.0 | 1.0 |
| DOFGBLLO_38 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_39 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_40 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_41 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_42 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_43 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_44 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_45 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_46 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_47 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_48 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_49 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_50 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_51 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_52 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_53 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_54 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_55 | 1.0 | 0.0 | 1.0 |
| DOFGBLLO_56 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_57 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_58 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_59 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_61 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_62 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_63 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_64 | 1.0 | 0.0 | 1.0 |
| DOFGBLLO_65 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_66 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_67 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_68 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_69 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_70 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_71 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_72 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_73 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_74 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_75 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_76 | 1.0 | 0.0 | 1.0 |
| DOFGBLLO_77 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_78 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_79 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_80 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_82 | 1.0 | 0.0 | 1.0 |
| DOFGBLLO_83 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_84 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_85 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_86 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_87 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_88 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_89 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_90 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_91 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_92 | 1.0 | 0.0 | 1.0 |
| DOFGBLLO_93 | 1.0 | 0.0 | 1.0 |
| DOFGBLLO_94 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_95 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_96 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_97 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_98 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_99 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_100 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_101 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_102 | 1.0 | 0.0 | 1.0 |
| DOFGBLLO_103 | 1.0 | 0.0 | 1.0 |
| DOFGBLLO_104 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_105 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_106 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_108 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_110 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_111 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_113 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_114 | 0.0 | 1.0 | 0.0 |
| DOFGBLLO_115 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DOFGBLLO_28 | 38050 | 64466 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| DOFGBLLO_34 | 3866 | 23075 | False | Siphoviridae | VOG4705, VOG3390, VOG4612, VOG4660, VOG4772, VOG0801, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4573, VOG4556, VOG1886 |
| DOFGBLLO_35 | 3999 | 27264 | False | Myoviridae | VOG4574, VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG1350, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG0815, VOG4553, VOG4555, VOG0720 |
| DOFGBLLO_68 | 15154 | 37081 | False | Siphoviridae | VOG4574, VOG4660, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG7930, VOG8610, VOG5008, VOG4705, VOG3390, VOG7718 |
Phigaro v2.4.0 (Database: Jan. 2024 release)