Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DAIOHMMI_1 | 2656363 | 2657217 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| DAIOHMMI_1 | 2637717 | 2638919 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| DAIOHMMI_1 | 2442657 | 2443826 | bmr | ARO:3003007 | bmr | 98.97 | 100.00 | M33768.1 |
| DAIOHMMI_1 | 1371729 | 1372046 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DAIOHMMI_1 | 1371391 | 1371729 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DAIOHMMI_1 | 910153 | 912723 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| DAIOHMMI_1 | 572622 | 574268 | vmlR | ARO:3004476 | vmlR | 98.72 | 100.18 | NC_000964.3 |
| DAIOHMMI_1 | 329517 | 330110 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| DAIOHMMI_1 | 278999 | 280432 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| DAIOHMMI_1 | 266183 | 267103 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DAIOHMMI_1 | 4172951 | 4174327 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | X08034 |
| DAIOHMMI_1 | 2656365 | 2657217 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| DAIOHMMI_1 | 266183 | 267103 | mph(K) | Spiramycin, Telithromycin | 97.94 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DAIOHMMI_1 | 4172954 | 4174327 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| DAIOHMMI_1 | 572625 | 574268 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.72 | 100.00 | WP_003234144.1 |
| DAIOHMMI_1 | 266183 | 267100 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| DAIOHMMI_1 | 2656366 | 2657217 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| DAIOHMMI_1 | 4170434 | 4170952 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 89.60 | 100.00 | WP_003242546.1 |
| DAIOHMMI_1 | 2050749 | 2053346 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DAIOHMMI_1 | 3209998 | 3210936 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| DAIOHMMI_1 | 3666593 | 3667774 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| DAIOHMMI_1 | 3202842 | 3209978 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.69 | 100.00 | NP_391076 |
| DAIOHMMI_1 | 3210964 | 3212583 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.63 | 100.00 | NP_391078 |
| DAIOHMMI_1 | 3666129 | 3666578 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| DAIOHMMI_1 | 3213834 | 3214619 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| DAIOHMMI_1 | 3664968 | 3666110 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| DAIOHMMI_1 | 2243271 | 2243912 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| DAIOHMMI_1 | 3212612 | 3213808 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| DAIOHMMI_1 | 2000704 | 2002467 | dep/capD | 0.0 | Capsule | Immune modulation | 98.64 | 100.00 | NP_389723 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DAIOHMMI_1 | 132850 | 133986 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DAIOHMMI_1 | 19075 | 19944 | pdxS | 3.12e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DAIOHMMI_1 | 1686662 | 1687438 | CodY | 3.41e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DAIOHMMI_1 | 3504237 | 3504806 | ClpP | 2.53e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DAIOHMMI_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DAIOHMMI_1 | 621908 | 624742 | False | Unknown | VOG8483, VOG9667, VOG9667, VOG0650 |
| DAIOHMMI_1 | 635620 | 658176 | False | Siphoviridae | VOG4681, VOG0796, VOG10227, VOG0720, VOG4564, VOG4555, VOG4553, VOG1125, VOG0815, VOG4623, VOG0816, VOG6394, VOG0724, VOG0799, VOG2621, VOG0725, VOG4605, VOG4599, VOG5535, VOG10940, VOG0753, VOG0136 |
| DAIOHMMI_1 | 1314254 | 1340421 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| DAIOHMMI_1 | 2193069 | 2217682 | False | Siphoviridae | VOG7718, VOG8610, VOG7930, VOG0275, VOG4052, VOG4910, VOG3389, VOG5042, VOG9712, VOG4626, VOG3390, VOG4705, VOG8440, VOG10230, VOG8537, VOG0054 |
| DAIOHMMI_1 | 3283670 | 3313082 | False | Siphoviridae | VOG4574, VOG0641, VOG4705, VOG3390, VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG4841, VOG0198, VOG1884, VOG0025, VOG9600, VOG11060, VOG4552 |
Phigaro v2.4.0 (Database: Jan. 2024 release)