safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	0	1	1.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
OJBNLLAE_1	1665192	1665896	WalR	2.04e-111	transcriptional regulatory protein	83.40	100.00	EPH95667

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
OJBNLLAE_4	44689	45750	repUS74	1062 / 1062	99.72	CP000424
OJBNLLAE_5	17193	18224	rep38	1040 / 1104	80.58	CP002655

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
OJBNLLAE_1	0.0	1.0	0.0
OJBNLLAE_2	1.0	0.0	1.0
OJBNLLAE_3	1.0	0.0	1.0
OJBNLLAE_4	1.0	0.0	1.0
OJBNLLAE_5	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
OJBNLLAE_1	127586	160843	False	Siphoviridae	VOG4606, VOG10904, VOG0226, VOG4841, VOG0195, VOG4581, VOG1886, VOG2935, VOG4556, VOG4568, VOG0205, VOG0207, VOG4586, VOG0209, VOG0660, VOG6163, VOG4605, VOG4599, VOG6455, VOG7896, VOG0753, VOG0703, VOG8294, VOG6495, VOG0275
OJBNLLAE_1	547825	583065	False	Siphoviridae	VOG4918, VOG0054, VOG7888, VOG4599, VOG4605, VOG4545, VOG0800, VOG0725, VOG0799, VOG0724, VOG5660, VOG0723, VOG4713, VOG1329, VOG1329, VOG4555, VOG4564, VOG0720, VOG4544, VOG0796, VOG4549, VOG4570, VOG0198, VOG3307, VOG5088, VOG3643, VOG3643, VOG0152, VOG4693, VOG6623, VOG9860, VOG0226, VOG4566, VOG0186, VOG1309, VOG8241, VOG1305, VOG6495, VOG9667
OJBNLLAE_1	828162	868964	False	Siphoviridae	VOG4918, VOG0703, VOG1637, VOG7896, VOG6455, VOG4599, VOG4605, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0562, VOG4553, VOG4573, VOG4556, VOG4844, VOG4841, VOG4549, VOG4570, VOG4892, VOG0198, VOG0044, VOG3643, VOG2820, VOG4643, VOG0275, VOG0195, VOG9759, VOG0327, VOG4606, VOG6545, VOG3777, VOG4548, VOG6394, VOG0322, VOG0321, VOG3642, VOG4967, VOG10867, VOG0181, VOG6450, VOG3622, VOG9667, VOG3479
OJBNLLAE_1	2571539	2608366	False	Siphoviridae	VOG9312, VOG2215, VOG6463, VOG4552, VOG0181, VOG10867, VOG4967, VOG3642, VOG0321, VOG9667, VOG0322, VOG0226, VOG4566, VOG0189, VOG9860, VOG0536, VOG0822, VOG9741, VOG0195, VOG0152, VOG3643, VOG0044, VOG0198, VOG7727, VOG4570, VOG10904, VOG4549, VOG1345, VOG4841, VOG4581, VOG1886, VOG4556, VOG4573, VOG4553, VOG9975, VOG0205, VOG0207, VOG4586, VOG0209, VOG0660, VOG6163, VOG4605, VOG4599, VOG6455, VOG7896, VOG1637, VOG0703